A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses

Luo, Jie; Chen, Yin; Wang, Heng-Hui; Zhu, Guoying; He, Peiyan; Guo, Jinchao; Yue, Lei; Chen, Zhongwen

doi:10.18632/oncotarget.18533

Cited by 19 publications

(17 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For analysis of circulating reservoirs, multiplex microbead-based immunoassays have been used to detect IgG antibodies for multiple pathogens 98 99. Multiplex, syndromic panels that include MERS-CoV have been demonstrated using PCR-based panels including MERS-CoV, showing similar limits of detection to single assays 89 100 101. Commercial respiratory panel tests including MERS-CoV have also recently been developed (see online supplementary table S1).…”

Section: Mers-cov Diagnosticsmentioning

confidence: 99%

An updated roadmap for MERS-CoV research and product development: focus on diagnostics

et al. 2019

View full text Add to dashboard Cite

Diagnostics play a central role in the early detection and control of outbreaks and can enable a more nuanced understanding of the disease kinetics and risk factors for the Middle East respiratory syndrome-coronavirus (MERS-CoV), one of the high-priority pathogens identified by the WHO. In this review we identified sources for molecular and serological diagnostic tests used in MERS-CoV detection, case management and outbreak investigations, as well as surveillance for humans and animals (camels), and summarised the performance of currently available tests, diagnostic needs, and associated challenges for diagnostic test development and implementation. A more detailed understanding of the kinetics of infection of MERS-CoV is needed in order to optimise the use of existing assays. Notably, MERS-CoV point-of-care tests are needed in order to optimise supportive care and to minimise transmission risk. However, for new test development, sourcing clinical material continues to be a major challenge to achieving assay validation. Harmonisation and standardisation of laboratory methods are essential for surveillance and for a rapid and effective international response to emerging diseases. Routine external quality assessment, along with well-characterised and up-to-date proficiency panels, would provide insight into MERS-CoV diagnostic performance worldwide. A defined set of Target Product Profiles for diagnostic technologies will be developed by WHO to address these gaps in MERS-CoV outbreak management.

show abstract

Section: Mers-cov Diagnosticsmentioning

confidence: 99%

An updated roadmap for MERS-CoV research and product development: focus on diagnostics

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The beads are separated within a Luminex analyzer and interrogated with two lasers- one for the identification of the bead and the other for quantification of bound reporter fluorophore 10. Similar technology is used in cancer research11 or for the simultaneous detection of several respiratory viruses 12…”

Section: Introductionmentioning

confidence: 99%

<p>Diagnosing Alpha-1-Antitrypsin Deficiency Using A PCR/Luminescence-Based Technology</p>

Veith¹,

Klemmer²,

Anton³

et al. 2019

COPD

View full text Add to dashboard Cite

PurposeAlpha-1-antitrypsin deficiency (AATD) is a rare hereditary condition resulting from the mutations in the SERPINA1 (serine protease inhibitor) gene and is characterized by low circulating levels of the alpha-1 antitrypsin (AAT) protein. The traditional algorithm for laboratory testing of AATD involves the analysis of AAT concentrations (nephelometry), phenotyping (isoelectric focusing, IEF), and genotyping (polymerase chain reaction, PCR); in selected cases, full sequencing of the SERPINA1 gene can be undertaken. New technologies arise that may make diagnosis easier and faster.MethodsWe developed and evaluated a new diagnostic algorithm based on Luminex xMAP (multi-analyte profiling) technology using Progenika A1AT Genotyping Test. In an initial learning phase, 1979 samples from individuals suspected of having AATD were examined by both, a traditional and a “new” algorithm. In a second phase, 1133 samples were analyzed with the Luminex xMAP only.ResultsBy introducing a Luminex xMAP based algorithm, we were able to simultaneously identify 14 mutations in SERPINA1 gene (instead of two- S and Z-by using our old algorithm). Although the quantity of IEF assays remained unchanged, the nephelometric measurements and sequencing were reduced by 79% and 63.4%, respectively.ConclusionThe new method is convenient, fast and user-friendly. The application of the Luminex xMAP technology can simplify and shorten the diagnostic workup of patients with suspected AATD.

show abstract

“…( Guo et al, 2013 ) and Streptococcus suis ( Bai et al, 2015 ). The Luminex-based array system is a multiplexed microsphere-based suspension system that offers a molecular diagnostic platform and provides an open and attractive approach for simultaneous, high-throughput and multiplex detection of up to 100 targets in protein and nucleic acid studies ( Yan et al, 2017 ). It has been approved by the US FDA for clinical diagnosis and has been used in various applications ( Dunbar, 2006 ; Liu et al, 2011 ; Glushakova et al, 2015 ; Silbereisen et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Molecular Serotyping Scheme and a Multiplexed Luminex-Based Array for Enterobacter aerogenes

Guo

Wang

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

Serotyping based on surface polysaccharide antigens is important for the clinical detection and epidemiological surveillance of pathogens. Polysaccharide gene clusters (PSgcs) are typically responsible for the diversity of bacterial surface polysaccharides. Through whole-genome sequencing and analysis, eight putative PSgc types were identified in 23 Enterobacter aerogenes strains from several geographic areas, allowing us to present the first molecular serotyping system for E. aerogenes. A conventional antigenic scheme was also established and correlated well with the molecular serotyping system that was based on PSgc genetic variation, indicating that PSgc-based molecular typing and immunological serology provide equally valid results. Further, a multiplex Luminex-based array was developed, and a double-blind test was conducted with 97 clinical specimens from Shanghai, China, to validate our array. The results of these analyses indicated that strains containing PSgc4 and PSgc7 comprised the predominant groups. We then examined 86 publicly available E. aerogenes strain genomes and identified an additional seven novel PSgc types, with PSgc10 being the most abundant type. In total, our study identified 15 PSgc types in E. aerogenes, providing the basis for a molecular serotyping scheme. From these results, differing epidemic patterns were identified between strains that were predominant in different regions. Our study highlights the feasibility and reliability of a serotyping system based on PSgc diversity, and for the first time, presents a molecular serotyping system, as well as an antigenic scheme for E. aerogenes, providing the basis for molecular diagnostics and epidemiological surveillance of this important emerging pathogen.

show abstract

A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses

Cited by 19 publications

References 40 publications

An updated roadmap for MERS-CoV research and product development: focus on diagnostics

An updated roadmap for MERS-CoV research and product development: focus on diagnostics

<p>Diagnosing Alpha-1-Antitrypsin Deficiency Using A PCR/Luminescence-Based Technology</p>

Establishment of a Molecular Serotyping Scheme and a Multiplexed Luminex-Based Array for Enterobacter aerogenes

Contact Info

Product

Resources

About