A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors

Mao, Rui; Ge, Ge; Wang, Zhuo; Hao, Rongzhang; Zhang, Guohao; Zhen-zhou, Yang; Lin, Bincheng; Ma, Yajun; Liu, Hongtao; Du, Yuguang

doi:10.1016/j.actatropica.2017.10.025

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…Loop-mediated isothermal ampli cation (LAMP), a novel method developed by Notomi et al is able to amplify DNA with great e cacy within an hour under isothermal conditions(9); it has been widely used as a diagnostic tool for several parasitic diseases (21,48). Nevertheless, the LAMP method has not been implemented in a large Chinese clinical study (19)(20)(21)(22). In this study, we have done so, and evaluated its speci city, sensitivity and clinical applicability for the detection of P. falciparum imported from Africa for the rst time.…”

Section: Discussionmentioning

confidence: 99%

“…Each detects symptomatic malaria cases with high sensitivity and speci city (13)(14)(15)(16)(17)(18). However, the evaluation of speci city and sensitivity of LAMP in large Chinese population in China has barely been reported (19)(20)(21)(22), perahaps because commercialized LAMP malaria diagnostic kits are not available in China (https://www.human.de/products/molecular-dx/malaria-lamp).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Falciparum Malaria Imported From Africa With a Highly Specific and Sensitive Loop-Mediated Isothermal Amplification (LAMP) Assay

Chen

Pan²,

Huang

et al. 2021

Preprint

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Background: Current malaria diagnostic tests, including microscopy and antigen-detecting rapid tests, cannot reliably detect low-density infections. Molecular methods such as polymerase chain reaction (PCR) are highly sensitive but remain too complex to be used in the ﬁeld. To complement existing diagnostic methods, a new LAMP assay for the detection and identification of Plasmodium falciparum imported from Africa was developed.Methods: A LAMP assay was developed to amplify the Actin I gene of P. falciparum. Microscopy, Nested PCR and the LAMP assay were conducted on 466 samples of suspected malaria patients imported from Africa to evaluate the new assay’s sensitivity and specificity. A plasmid construct, cultured P. falciparum, and the clinical samples were used to evaluate the the limit of detection (LOD). Results: Compared to Nested PCR, the sensitivity and specificity of the nove developed LAMP were 100 % (95% CI 98.54 - 100%) and 99.07 % (95% CI 96.68 - 99.89%) respectively. The LAMP assay was found to be highly sensitive, with the detection limit as low as 102 copies/μL and 10-fold higher detection limit than single PCR when performed on serial dilutions of the plasmid construct. The LAMP assay detected 0.01 parasites/μL, when cultured P. falciparum was used as template. The novel LAMP assay detected 1-7 parasites /μL blood in clinical samples，which is more sensitively than the commercial product (Loopamp MALARIA Pan/Pf detection kit; Eiken Chemical Co., Tokyo, Japan) for clinical samples detaction of P. falciparum, which detected >20 parasites /μL blood sample. Conclusion:For the first time, the Actin I gene of P. falciparum was used as target for LAMP primers to detect imported P. falciparum from Africa imported to China. The novel, highly specific and sensitive LAMP assay is a practical and effective diagnostic method for detecting low-density P. falciparum infections imported from Africa. For the first time, such LAMP primers were evaluated by using a plasmid construct, cultured P. falciparum and clinical samples at same work plafom, which offer excellent means of assay validation and should be considered in future studies of this kind.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Falciparum Malaria Imported From Africa With a Highly Specific and Sensitive Loop-Mediated Isothermal Amplification (LAMP) Assay

Chen

Pan²,

Huang

et al. 2021

Preprint

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“…In recent years, more and more targets for detection on diagnostic chips have been investigated. Some salient examples thereof are circulating tumor cells (CTC) of various types of cancer [1,[36][37][38], rare cells (e.g., sickle-cell variants of red blood cells) [39,40], parasites, like Plasmodium falciparum [1,7,10,11,[13][14][15][21][22][23]36,[41][42][43][44][45][46][47][48][49][50] and Trypanosoma spp. [8,44,[51][52][53][54][55][56][57] and even plant pathogens [26,58], as well as-after cells have been lysed-subcellular infection markers (e.g., DNA, RNA fragments) [10,11,22,43,[59][60][61][62].…”

Section: What We Can Detectmentioning

confidence: 99%

“…An alternative technique for diagnosing sub-cellular targets is loop-mediated isothermal amplification (LAMP), which can run at a single temperature of around 65 • C and inside a single tube where the cell lysate is mixed with DNA polymerase and a set of four (or more) specifically designed primers [214]. The fact that it needs less equipment to be run, and that it can even be more portable [55], or fully non-instrumented [48], makes LAMP and its derivatives very promising for use in the field for resource-scarce settings [11,22,47,48,55,61].…”

Section: Polymerase Chain Reaction (Pcr Nested Pcr Qpcr/ Rt-pcr)mentioning

confidence: 99%

“…Point-of-Care (POC) means that these diagnostic devices can be used at the patient level and deliver a diagnosis locally and quickly-without need for taking samples, sending them away for analysis and having to continue care while waiting on the outcome of the diagnostic test. This is often achieved with the LAMP test and its derivatives, LAMPport and NINA-LAMP [22,47,48,55,61]. However, many other, especially microfluidic, approaches have also been tested and discussed for their aptitude to be used for POC diagnostic tools [38,45,205,212,[221][222][223][224][225].…”

Section: Point-of-care Diagnostics (Poc)mentioning

confidence: 99%

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Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

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Development of a film-based immunochromatographic microfluidic device for malaria diagnosis

Choi

Cho²,

Kim

et al. 2019

Biomed Microdevices

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A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors

Cited by 18 publications

References 30 publications

Detection of Falciparum Malaria Imported From Africa With a Highly Specific and Sensitive Loop-Mediated Isothermal Amplification (LAMP) Assay

Detection of Falciparum Malaria Imported From Africa With a Highly Specific and Sensitive Loop-Mediated Isothermal Amplification (LAMP) Assay

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Development of a film-based immunochromatographic microfluidic device for malaria diagnosis

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