A Multiplex PCR Assay for the Detection of Food-borne Pathogens in Meat Products

Kim, Hyoun Wook; Kim, Ji Hyun; Rhim, Seong Ryul; Lee, Kyung A.; Kim, Cheon Jei; Paik, Hyun Dong

doi:10.5851/kosfa.2010.30.4.590

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…Current methods for the detection of food-borne pathogens generally involve the following: (a) colony isolation on selective media, (b) the use of biochemical tests, and (c) serotyping using antibodies against specific bacterial antigens. However, these traditional culture-based methods are both cumbersome and time consuming ( Kim et al ., 2010 ). Recently, a polymerase chain reaction (PCR) method has been developed and applied frequently for detection of pathogenic microorganisms due to its rapidity and more feasibility for industrial application.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in Low-fatted Milk by Multiplex PCR

Kim¹,

Rhim²,

Kim³

et al. 2014

Korean Journal for Food Science of Animal Resources

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A rapid and specific PCR assay for the simultaneous detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. Multiplex PCR developed in this study produced only actA, fliC, hbl, invA, ileS amplicons, but did not produce any non-specific amplicon. The primer sets successfully amplified the target genes in the multiplex PCR without any non-specific or additional bands on the other strains. The multiplex PCR assays also amplified some target genes from five pathogens, and multiplex amplification was obtained from as little as 1 pg of DNA. According to the results from the sensitivity evaluation, the multiplex PCR developed in this study detected 10 cells/mL of the pathogens inoculated in milk samples, respectively. The results suggested that multiplex PCR was an effective assay demonstrating high specificity for the simultaneous detection of five target pathogens in food system.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in Low-fatted Milk by Multiplex PCR

Kim¹,

Rhim²,

Kim³

et al. 2014

Korean Journal for Food Science of Animal Resources

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Physical, textural and sensory characteristics of legume-based gluten-free muffin enriched with waxy rice flour

Jeong

Chung

2018

Food Sci Biotechnol

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Physical, textural, and sensory characteristics of the gluten-free muffins made of mungbean and cowpea flours enriched with waxy rice flour were investigated. Amylose content and pasting viscosity of legume-waxy rice flour blend (1:1) were lower than that of wheat flour. The number and area of gas cells of muffin containing legume flour was lower than that of control muffin with wheat flour. Legume-based muffins had low lightness value due to the presence of color components. Weight of the muffins containing legume was higher than that of control muffin, but their height and volume were lower than control. Legume-based muffins had greater crude ash and protein contents than control. Compared with control, muffin containing legume flour had significantly higher hardness and gumminess. In sensory evaluation, overall acceptance of muffin containing Okdang cultivar of cowpea was the highest among legume-based muffins, which was comparable to control muffin.

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Simultaneous diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections by multiplex PCR

Hong¹,

Lee²,

Kim³

et al. 2014

Korean Journal of Veterinary Service

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The economic impact of swine mycoplasma infection is high. An accurate diagnosis is often difficult and time consuming. We report the development and validation of an effective multiplex polymerase chain reaction (PCR) assay that detects Mycoplasma (M.) hyopneumoniae and M. hyorhinis. The multi detection of M. hyopneumoniae and M. hyorhinis primer set were employed to detect mycoplasma species and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by M. hyopneumoniae and M. hyorhinis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were used to evaluate the specificity of the multiplex assay. The corresponding specific DNA products were amplified for each pathogen. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. hyopneumoniae and M. hyorhinis.

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A Multiplex PCR Assay for the Detection of Food-borne Pathogens in Meat Products

Cited by 3 publications

References 35 publications

Simultaneous Detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in Low-fatted Milk by Multiplex PCR

Simultaneous Detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in Low-fatted Milk by Multiplex PCR

Physical, textural and sensory characteristics of legume-based gluten-free muffin enriched with waxy rice flour

Simultaneous diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections by multiplex PCR

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