A multiplex-PCR assay for identification of the quarantine plant pathogen Xanthomonas axonopodis pv. phaseoli

Boureau, Tristan; Kerkoud, Mohamed; Chhel, Fabien; Hunault, Gilles; Darrasse, Armelle; Brin, Chrystelle; Durand, Karine; Hajri, Ahmed; Poussier, Stéphane; Manceau, Charles; Lardeux, Frédéric; Saubion, Frédéric; Jacques, Marie‐Agnès

doi:10.1016/j.mimet.2012.10.012

Cited by 33 publications

(35 citation statements)

References 25 publications

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“…It addresses the problem by involving more general or multiplex approaches as necessary. Multiplex PCR and serological assays have been developed for microbial forensics and biosecurity applications (Boureau et al 2013;Charlermroj et al 2013).…”

Section: Assay Featuresmentioning

confidence: 99%

Plant Disease Diagnostics for Forensic Applications

Fletcher¹,

Ochoa-Corona²,

Payton

2014

Detection and Diagnostics of Plant Pathogens

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Although most plant diseases are the result of natural or unintentional causes, cropping systems and essential natural plant resources such as forests and grasslands also are considered vulnerable to actions of nefarious intent. Microbial forensics is defined as the application of scientific approaches to solving a crime that involves a microorganism; its goal is to investigate and present unbiased scientific evidence useful for attributing the crime to a perpetrator. Recent programs intended to enhance general capabilities in microbial forensics have included specific attention to plant pathogens. Compared to the strategies employed by traditional plant disease diagnosticians, forensic applications of plant pathogen diagnostics require unusually high levels of stringency, reliability, and prior validation. These assays must be paired with court-defensible sampling methods, chain of custody, and other traditional and non-traditional methods of forensic investigation.

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Section: Assay Featuresmentioning

confidence: 99%

Plant Disease Diagnostics for Forensic Applications

Fletcher¹,

Ochoa-Corona²,

Payton

2014

Detection and Diagnostics of Plant Pathogens

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show abstract

“…Des caractérisations dans le domaine de la biologie végétale ont donc été réalisées, et ont conduit au dépôt d'un brevet pour le dépistage du pathovar phaseoli de Xanthomonas Axonopodis [3]. Ce test vient d'être validé au niveau européen comme un des tests officiels de dépistage de ce pathovar.…”

Section: Expérimentationsunclassified

“…Il s'agit ainsi de trouver la plus petite combinaison de gènes de virulence spécifiques au Xap. Cette combinaison peut être utilisée pour concevoir un test PCR multiplexe pour l'identification de Xap [2,3], dont le coût est directement lié au nombre de gènes à tester. Les résultats obtenus montrent que la combinaison des tests moléculaires ainsi obtenus fournit une technique rapide pour l'identification de toutes les souches de Xanthomonas pathogènes sur les haricots.…”

Section: Introductionunclassified

Application du problème de caractérisation multiple à la conception de tests de diagnostic pour la biologie végétale

Chhel¹,

Lardeux²,

Saubion³

et al. 2013

Revue d'intelligence artificielle

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“…Multiplex PCR utilizes a single reaction with several primer pairs, each generating an amplicon that can be separated and visualized through electrophoresis 17 . This technique has been extensively applied in plant pathology as it permits the identification of more than one target in a single reaction 6,18,19 . PCR-based methods have been utilized worldwide in order to identify and detect P. palmivora in orchids 20 , in important Australian plants 5 , in coconut 21 , and in citrus 22 .…”

Section: Introductionmentioning

confidence: 99%

Development of a multiplex PCR assay for rapid and simultaneous detection of rubber tree pathogens Phytophthora spp. and P. palmivora

Chirapongsatonkul¹,

U-taynapun²,

Chanwun³

et al. 2015

ScienceAsia

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Phytophthora spp. cause devastating effects on crops and natural ecosystems worldwide. In Thailand, P. palmivora is an important pathogenic species of the rubber plant (Hevea brasiliensis), reducing latex production. This study aimed to develop a PCR-based method to rapidly and simultaneously detect the genus Phytophthora and P. palmivora. A single round nested multiplex PCR with 4 primers, Phy1s/Phy2a/Pal1s/Pal2a, and a single round seminested multiplex PCR using 3 primers, Phy1s/Phy2a/Pal2a, were established. The obtained PCR products of 1025 bp and 650 bp represent the ITS1-5.8S-ITS2-28S region of the genus Phytophthora and P. palmivora, respectively. By using various amounts of DNA of a positive strain P. palmivora KBNM 9 and the vector containing ITS region as templates, the sensitivity of these methods was compared to the simple PCR. The single round semi-nested multiplex PCR was applied for the detection of P. palmivora in infected rubber leaves and soil as well as the leaves and bark collected from planted areas. The results of application and the sensitivity test using the DNA of P. palmivora mycelium are in agreement. Only one band of 650 bp representing the genus Phytophthora and P. palmivora was detected at low template number. The DNA from severe symptom-exhibiting and dehydrated leaves, however, showed negative results comparable to the classical isolation method that showed no Phytophthora and P. palmivora growth on selective media. This technique may be used to detect and manage Phytophthora infection in important crops.

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A multiplex-PCR assay for identification of the quarantine plant pathogen Xanthomonas axonopodis pv. phaseoli

Cited by 33 publications

References 25 publications

Plant Disease Diagnostics for Forensic Applications

Plant Disease Diagnostics for Forensic Applications

Application du problème de caractérisation multiple à la conception de tests de diagnostic pour la biologie végétale

Development of a multiplex PCR assay for rapid and simultaneous detection of rubber tree pathogens Phytophthora spp. and P. palmivora

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