A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region

Rodrigues, Elzemar Martins Ribeiro; Leite, Fabio Pereira das Neves; Hutz, Mara H.; Palha, Teresinha de Jesus Brabo Ferreira; Ribeiro-dos-Santos, Ândrea; Santos, Sidney Emanuel Batista dos

doi:10.1016/j.fsigen.2007.10.179

Cited by 28 publications

(6 citation statements)

References 22 publications

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“…The D18S51 and FGA loci showed higher discrimination powers of 0.973 and 0.976, respectively. Our results for the Brazilian population (Grattapaglia et al, 2001;Fridman et al, 2008;Aguiar et al, 2012) agreed with those for the populations of Pará -North Region (Ribeiro-Rodrigues et al, 2008), the Mato Grosso do Sul -Midwest Region (Silva et al, 2004), Minas Gerais -Southeast Region (del Castillo et al, 2009), and Rio Grande do Sul -South Region (Leite et al, 2003) for most markers and indicators assessed.…”

Section: Resultssupporting

confidence: 88%

Distribution of forensic marker allelic frequencies in Pernambuco, Northestern Brazil

Santos¹,

Souza²,

Rabêlo³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Pernambuco is one of the 27 federal units of Brazil, ranking seventh in the number of inhabitants. We examined the allele frequencies of 13 short tandem repeat loci (CFS1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, vWA, andTPOX), the minimum recommended by the Federal Bureau of Investigation and commonly used in forensic genetics laboratories in Brazil, in a sample of 609 unrelated individuals from all geographic regions of Pernambuco. The allele frequencies ranged from 5 to 47.2%. No significant differences for any loci analyzed were observed compared with other publications in other various regions of Brazil. Most of the markers observed were in Hardy-Weinberg equilibrium. The occurrence of the allele 47.2 (locus FGA) and alleles 35.1 and 39 (locus D21S11), also described in a single study of the Brazilian population, was observed. The other forensic parameters analyzed (matching probability, power of discrimination, polymorphic information content, paternity exclusion, complement factor I, observed heterozygosity, expected heterozygosity) indicated that the studied markers are very informative for human forensic identification purposes in the Pernambuco population.

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Section: Resultssupporting

confidence: 88%

Distribution of forensic marker allelic frequencies in Pernambuco, Northestern Brazil

Santos¹,

Souza²,

Rabêlo³

et al. 2015

Genet. Mol. Res.

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“…Samples were genotyped for 12 X-STR markers (DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, D X S 7 1 3 3 , G ATA 1 7 2 D 0 5 , D X S 7 1 3 0 , H P RT B , GATA31E08, DXS7423, and DXS10011) in a single multiplex reaction. Primers and PCR conditions were as described elsewhere [7,8]. PCR products were submitted to capillary electrophoresis, while separation and detection of fragments were performed with an ABI PRISM 3130 Genetic Analyzer using the GS-500 ROX size standard, filter set D, and POP7 polymer (Applied Biosystems, Foster City, CA, USA).…”

Section: Dna Analysismentioning

confidence: 99%

“…However, in some complex cases, autosomal markers may be inconclusive [2], requiring the use of non-autosomal markers, like those found in X and Y chromosomes. In recent years, many studies built and validated panels of STRs markers on the X chromosome (X-STR markers) to be used in forensic genetics, usually employing polymerase chain reaction (PCR) multiplexes ranging from six to 12 STR per reaction [3][4][5][6][7][8]. Such panels have been useful in complex relatedness cases when [9][10][11] (1) the putative father was not available, (2) the relatives were consanguineous, (3) the relative of the putative father is either his mother or daughter, and (4) in maternity investigation of a son [12].…”

Section: Introductionmentioning

confidence: 99%

“…Despite extensive availability of autosomal [13] and Ychromosome [14] markers databases in Brazil, there are only few and non-representative X-STR markers databases [6,7,15,16], mostly restricted to its Southeastern region [12]. Recently, we developed a 12 X-STR multiplex panel to estimate the genetic variability of Brazilian populations [8].…”

Section: Introductionmentioning

confidence: 99%

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Extensive survey of 12 X-STRs reveals genetic heterogeneity among Brazilian populations

Ribeiro-Rodrigues

Palha

Bittencourt³

et al. 2011

Int J Legal Med

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The admixed Brazilian population shows high levels of genetic variability, which resulted from the contribution of three main ethnicities, Amerindian, European, and African. However, due to its huge territory, admixing has been asymmetrical, i.e., the relative contribution from each ethnicity has been unequal in the five geopolitical regions of the country. The aim of this study was to describe genetic variability using a panel of short-tandem repeats on the X chromosome (X-STR) in order to perform a comprehensive evaluation of the usefulness of such markers for forensic purposes in Brazil. Twelve X-STR (DXS9895, DXS7132, DXS6800, DXS9898, DXS6789, DXS7133, GATA172D05, DXS7130, HPRTB, GATA31E08, DXS7423, and DXS10011) were chosen and tested in a sample of 2,234 individuals belonging to 16 out of the 27 Brazilian States, representing all of its five geopolitical regions. No markers showed significant deviation from the Hardy-Weinberg equilibrium, even when analyses were partitioned to represent geopolitical regions. Genetic diversity per locus ranged from 67% (DSX7133) to 95% (DXS10011), and the State of Ceará showed the highest average genetic diversity (79% for all 12 X-STR markers). Considering the Brazilian population as a whole, the power of discrimination of the 12 X-STR panel in females (PDF) was 0.999999999999994, while the power of discrimination in males (PDM) was 0.9999999969. Such high values suggest the potential of that panel to be used in forensic applications and relatedness tests among individuals. Comparisons among the Brazilian populations investigated revealed significant differences when they were compared among each other, a pattern that was maintained when additional populations from Europe and Latin America were compared to Brazilians. Our results highlight the need and usefulness of specific genetic database for forensic purposes in Brazilian populations.

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“…Only three kits were available for the analysis of these markers: Mentype® Argus X-UL (Biotype AG), Mentype® Argus X-8 (Biotype AG) and finally, the Investigator Argus X-12 (Qiagen), considered the latest technology in X-STR testing [5]. However, in Brazil, population genetic data was reported for 42 X-STRs [6,7], especially markers of the 10-plex system implemented by Gusmão et al [8] and the 12-plex system developed by Ribeiro-Rodrigues et al [9,10]. This data is observed in 20 of the 27 Brazilian States (including Federal District); the Northeast and Midwest were the least studied regions and there is no information for the State of Mato Grosso (Midwest).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of an X-STR decaplex system in the State of Mato Grosso, Brazil: distribution, forensic efficiency and population structure

Martins

Oliveira-Brancati

et al. 2017

Int J Legal Med

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Studies with X-STR loci show population genetic substructure, which makes necessary the characterization of such markers in the different geographical and/or ethnic populations. Therefore, this study assessed the distribution and forensic efficiency of an X-STR decaplex system in the population of the State of Mato Grosso, as well as analysed the population structure of this State based on the aforementioned system. All X-STR markers were in Hardy-Weinberg equilibrium and linkage equilibrium, and the DXS6809 was the most informative marker. The power of discrimination value in females and males was 0.99999999995 and 0.9999994, respectively. Analysis of molecular variance indicated 1.10% (p < 0.00001) of heterogeneity among Europeans, Africans, Brazilians and other Latin Americans, and in relation to such groups, the population of the State of Mato Grosso showed lower genetic variation when compared with the Brazilian group (-0.10%, p = 0.67327). The genetic distance analysis showed lower values of F (0.0004 ≤ F ≤ 0.00331), with non-significant p value (p > 0.00024), between the populations of Mato Grosso and Mato Grosso do Sul, Paraná and the Southeast region of Brazil (except for one sample of Rio de Janeiro). F values with significant p values (p ≤ 0.00024) were obtained between the population of Mato Grosso and Iberian, African and some Latin American populations. The X-STR decaplex system proved to be extremely useful in the population of the State of Mato Grosso, and the data obtained does not show the need for a specific forensic database for this State in relation to the Brazilian populations compared in this study, except for population of Rio de Janeiro.

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A multiplex PCR for 11 X chromosome STR markers and population data from a Brazilian Amazon Region

Cited by 28 publications

References 22 publications

Distribution of forensic marker allelic frequencies in Pernambuco, Northestern Brazil

Distribution of forensic marker allelic frequencies in Pernambuco, Northestern Brazil

Extensive survey of 12 X-STRs reveals genetic heterogeneity among Brazilian populations

Genetic characterization of an X-STR decaplex system in the State of Mato Grosso, Brazil: distribution, forensic efficiency and population structure

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