A multiplex PCR method to identify bushmeat species in wildlife forensics

Morf, Nadja; Wood, K.L.; Köppel, René; Felderer, Nora; Daniels, Mats; Tenger, B.; Krätzer, A.

doi:10.1016/j.fsigss.2013.10.104

Cited by 9 publications

(3 citation statements)

References 4 publications

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“…Forensic genetics is becoming the key to fighting illegal wildlife trafficking [18] and establishing national and international legislation to protect the biodiversity of habitats and species [15,16]. This is also deeply intertwined with the protection of the legal economic chain, passing through the meticulous control of biologically derived products, to identify the wild meat belonging to a particular or protected species [112,113] and control the correct food composition in raw materials such as cheese or meat [114].…”

Section: Wildlife In Forensic Geneticsmentioning

confidence: 99%

The Revolution of Animal Genomics in Forensic Sciences

Cardinali

Tancredi

Lancioni

2023

IJMS

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Nowadays, the coexistence between humans and domestic animals (especially dogs and cats) has become a common scenario of daily life. Consequently, during a forensic investigation in civil or criminal cases, the biological material from a domestic animal could be considered “evidence” by law enforcement agencies. Animal genomics offers an important contribution in attacks and episodes of property destruction or in a crime scene where the non-human biological material is linked to the victim or perpetrator. However, only a few animal genetics laboratories in the world are able to carry out a valid forensic analysis, adhering to standards and guidelines that ensure the admissibility of data before a court of law. Today, forensic sciences focus on animal genetics considering all domestic species through the analysis of STRs (short tandem repeats) and autosomal and mitochondrial DNA SNPs (single nucleotide polymorphisms). However, the application of these molecular markers to wildlife seems to have gradually gained a strong relevance, aiming to tackle illegal traffic, avoid the loss of biodiversity, and protect endangered species. The development of third-generation sequencing technologies has glimmered new possibilities by bringing “the laboratory into the field”, with a reduction of both the enormous cost management of samples and the degradation of the biological material.

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Section: Wildlife In Forensic Geneticsmentioning

confidence: 99%

The Revolution of Animal Genomics in Forensic Sciences

Cardinali

Tancredi

Lancioni

2023

IJMS

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“…Fresh meat and plants used as reference and standard material were obtained from local suppliers and the expected DNA sequence for this species was verified by sequencing [18] or specific real-time PCR (e.g. 19).…”

Section: Meat and Plant Samplesmentioning

confidence: 99%

“…Performance of AllVeg.CThe correlation and amplification efficiencies were taken from the Rotorgene algorithm directly and the performance was calculated[18] …”

mentioning

confidence: 99%

Detection of animal DNA in vegan food by multiplex qPCR system

Köppel¹,

Lederman²,

Velsen³

et al. 2020

Eur Food Res Technol

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Vegan food claims to be free of all animal material. This must be verified by the food control authority and the production control laboratory. The method must be sensitive and able to detect animal material after processing such as pasteurization and storage. We have, therefore, developed a multiplex qPCR to simultaneously detect animal, fish and plant DNA in food samples. The system is cost-efficient and exhibited a high sensitivity. The specificity tests confirmed that all relevant species are detected. The plant-specific system served as a DNA isolation control and was used to generate valid results. A large selection of market samples had been analyzed and showed the expected results and the robustness of this analytical tool in routine analysis.

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