A multiplex PCR-SSCP test to genotype bovine β-casein alleles A1
, A2
, A3
, B, and C

Barroso, Ángel Barturen; Dunner, S.; Cañón, Javier

doi:10.1046/j.1365-2052.1999.00445-6.x

Cited by 16 publications

(10 citation statements)

References 5 publications

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“…Allele sizes were analysed with the Allelelinks program (Amersham Pharmacia Biotech, Europe) and standardized to internationally agreed sizes. The microsatellites were: BM1818, BM1824, BM2113, CSSM66, ETH10, ETH152, ETH185, HEL1, HEL5, HEL13, ILSTS6, ILSTS73, INRA23, INRA37, INRAK, SPS115, TGLA122, TGLA126, TGLA227 andURB58. Different methods were used in the genotyping of seven milk protein loci (αS 1 -casein promoter (CSN1S1Prom) (Prinzenberg et al, 2003), αS 1 -casein (CSN1S1) (Jann et al, 2002), αS 2 -casein (CSN1S2) (Prinzenberg, 1998), β-casein (CSN2) (Barroso et al, 1999), κ-casein (CSN3) (Prinzenberg et al, 1999), α-lactalbumin (LAA) (Mitra et al, 1998) and β-lactoglobulin (LGB) (Medrano and Aguilar-Cordova, 1990)) and five blood protein loci (albumin (ALB), carbonic anhydrase (CA II), vitamin D-binding protein (GC), haemoglobin (HBB) and transferrin (TF)) by the procedures of Erhardt (1986Erhardt ( , 1991.…”

Section: Animals Markers and Genotypingmentioning

confidence: 99%

An evaluation of genetic diversity indices of the Red Bororo and White Fulani cattle breeds with different molecular markers and their implications for current and future improvement options

Ibeagha‐Awemu

Erhardt

2006

Trop Anim Health Prod

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The genetic diversity of the Red Bororo and White Fulani cattle breeds of Cameroon and Nigeria was assessed with a panel of 32 markers. Estimates for the various indices of genetic diversity, total number of alleles (TNA), mean observed number of alleles (MNA), mean effective number of alleles (MNE), observed heterozygosity (Hob) and expected heterozygosity (Hex), were higher at microsatellite loci than at protein loci. Mean Hex values were above 71% at microsatellite loci in all the breeds and ranged from 37% to 41.6% at milk protein loci and from 40.9% to 45.6% at blood protein loci. The highest TNA and MNA of microsatellites were recorded for the Nigerian White Fulani. MNE of milk protein loci was highest in the Cameroonian Red Bororo, while TNA of blood protein loci was highest in the Cameroonian White Fulani. The high genetic diversity levels indicate the presence of the necessary ingredients for improvement breeding and conservation. Multi-locus estimates of within-population inbreeding (f), total inbreeding (F) and population differentiation (theta) of the breeds were significantly different from zero, except for theta of blood proteins. A high level of gene flow was found between the breeds (5.829). The phylogenetic relationship existing among the four breeds is greatly influenced by location. The high gene flow between the breeds may lead to a loss of genetic diversity through genetic uniformity and a reduction in opportunities for future breed development. We propose an improvement scheme with aims to prevent loss of genetic diversity, improve productivity and reduce uncontrolled genetic exchanges between breeds.

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Section: Animals Markers and Genotypingmentioning

confidence: 99%

An evaluation of genetic diversity indices of the Red Bororo and White Fulani cattle breeds with different molecular markers and their implications for current and future improvement options

Ibeagha‐Awemu

Erhardt

2006

Trop Anim Health Prod

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“…Alleles A and B of LAA and LGB were investigated by PCR-RFLP according to, respectively, Mitra et al (1998) and Medrano & Aguilar-Cordova (1990). PCR-SSCP analysis was used to investigate variations at the CSN1S1-5k promoter region (CSN1S1Prom) (promoter alleles 1 to 4, Prinzenberg et al 2003), CSN1S1 (alleles B and C, Jann et al 2002), CSN2 (alleles A 1 , A 2 , A 3 , B, C and I, Barroso et al 1999;Jann et al 2001) and CSN3 (alleles A, A 1 , B, C, E, F, G, H and I, Prinzenberg et al 1999) genes. Allele-specific PCR and PCR-RFLP were used to determine the presence of, respectively, CSN1S1 F (Prinzenberg et al 1998) and CSN1S1 A (Prinzenberg & Erhardt, 1994).…”

Section: Genotyping Of Allelesmentioning

confidence: 99%

High variability of milk protein genes in Bos indicus cattle breeds of Cameroon and Nigeria and characterization of a new α_s1-casein promoter allele

Ibeagha‐Awemu

Prinzenberg

Erhardt

2005

Journal of Dairy Research

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The study provides the first comprehensive information on the variability of milk protein genes of Bos indicus and Bos taurus cattle breeds in Cameroon and Nigeria. The investigations indicate a high diversity of milk protein genes for the zebu populations. Of the investigated alleles, 21 out of 29 were observed. The method of single strand conformation polymorphism (SSCP) was a particularly useful technique because it allowed discrimination of alleles, including zebu-specific alleles at the CSN2 (I) and CSN3 (AI and H) loci, not separated by protein electrophoretic techniques and also made possible the detection of a further CSN1S1 5′ promoter allele (CSN1S1Prom5), which is also zebu-specific. Characterization of CSN1S1Prom5 showed that it was the most variable of all described CSN1S1 promoter alleles. A potential GATA consensus motif is created by mutations in CSN1S1Prom5. Intra-breed diversity measured as mean effective number of alleles was higher in the zebu populations than in the taurine breeds. Of the expected casein haplotypes, 96 out of 320 were present in the studied breeds. 2-C-A-A2-H (CSN1S1Prom2-CSN1S1C-CSN1S2A-CSN2A2-CSN3H) and 5-C-A-A2-H were zebu-specific while 1-B-A-A2-B was specific to the taurines. Overall distribution of alleles and haplotypes clearly separated the zebu populations from the taurine breeds. Zebu influence on the taurine breed Namchi was detected through the occurrence of zebu alleles and haplotypes. High variability of milk proteins also means availability of resources for breed development, phylogenetic studies, and conservation and management decisions.

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“…The β-casein (CSN2) and κ-casein (CSN3) genes were genotyped by PCR-SSCP which differentiates alleles which cannot be identified by isoelectric focusing at the protein level. The techniques used differentiate the CSN2 alleles A 1 , A 2 , A 3 , B, C, and I [6], and the CSN3 alleles A, A I , B, C, E, F, G, H, and I [38], respectively.…”

Section: Genotyping Of Casein Polymorphismsmentioning

confidence: 99%

Geographic distribution of haplotype diversity at the bovine casein locus

et al. 2004

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Abstract

The genetic diversity of the casein locus in cattle was studied on the basis of haplotype analysis. Consideration of recently described genetic variants of the casein genes which to date have not been the subject of diversity studies, allowed the identification of new haplotypes. Genotyping of 30 cattle breeds from four continents revealed a geographically associated distribution of haplotypes, mainly defined by frequencies of alleles at CSN1S1 and CSN3. The genetic diversity within taurine breeds in Europe was found to decrease significantly from the south to the north and from the east to the west. Such geographic patterns of cattle genetic variation at the casein locus may be a result of the domestication process of modern cattle as well as geographically differentiated natural or artificial selection. The comparison of African Bos taurus and Bos indicus breeds allowed the identification of several Bos indicus specific haplotypes (CSN1S1*C-CSN2*A²-CSN3*A_I/CSN3*H) that are not found in pure taurine breeds. The occurrence of such haplotypes in southern European breeds also suggests that an introgression of indicine genes into taurine breeds could have contributed to the distribution of the genetic variation observed.

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A multiplex PCR-SSCP test to genotype bovine β-casein alleles A¹ , A² , A³ , B, and C

Cited by 16 publications

References 5 publications

An evaluation of genetic diversity indices of the Red Bororo and White Fulani cattle breeds with different molecular markers and their implications for current and future improvement options

An evaluation of genetic diversity indices of the Red Bororo and White Fulani cattle breeds with different molecular markers and their implications for current and future improvement options

High variability of milk protein genes in Bos indicus cattle breeds of Cameroon and Nigeria and characterization of a new α_s1-casein promoter allele

Geographic distribution of haplotype diversity at the bovine casein locus

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A multiplex PCR-SSCP test to genotype bovine β-casein alleles A1 , A2 , A3 , B, and C

Cited by 16 publications

References 5 publications

An evaluation of genetic diversity indices of the Red Bororo and White Fulani cattle breeds with different molecular markers and their implications for current and future improvement options

An evaluation of genetic diversity indices of the Red Bororo and White Fulani cattle breeds with different molecular markers and their implications for current and future improvement options

High variability of milk protein genes in Bos indicus cattle breeds of Cameroon and Nigeria and characterization of a new αs1-casein promoter allele

Geographic distribution of haplotype diversity at the bovine casein locus

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Product

Resources

About

A multiplex PCR-SSCP test to genotype bovine β-casein alleles A¹ , A² , A³ , B, and C

High variability of milk protein genes in Bos indicus cattle breeds of Cameroon and Nigeria and characterization of a new α_s1-casein promoter allele