A Multiplex PCR Test for Determination of Mating Type Applied to the Plant Pathogens Tapesia yallundae and Tapesia acuformis

Dyer, Paul S.; Furneaux, Peter A.; Douhan, Greg W.; Murray, T. D.

doi:10.1006/fgbi.2001.1279

Cited by 51 publications

(26 citation statements)

References 35 publications

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“…In contrast, DNA-based approaches are relatively inexpensive and usually provide reliable mating type assignments (Cherif et al 2006;Dyer et al 2001;Yokoyama et al 2004). These DNA-based methods are, however, dependent on the availability of sequence information for the MAT locus, because MAT idiomorph-specific PCR assays exploit the inherent differences in the MAT genes (Dyer et al 2001;Steenkamp et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

et al. 2012

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In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses on agar plates, the heterothallic nature of the wood-inhabiting fungus Ophiostoma quercus was confirmed and mating types were assigned to 10 isolates. Primers were subsequently designed to target both the mating type 1 (MAT1-1) and 2 (MAT1-2) idiomorphs in these isolates. The results showed that all isolates contained sequence fragments representing both idiomorphs. This was unexpected, as each isolate from a heterothallic species would typically contain only one of the two possible MAT idiomorphs. An atypical mating system such as the one described in this study has not previously been encountered in any other Ascomycota mating locus described to date.

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Section: Introductionmentioning

confidence: 99%

“…These DNA-based methods are, however, dependent on the availability of sequence information for the MAT locus, because MAT idiomorph-specific PCR assays exploit the inherent differences in the MAT genes (Dyer et al 2001;Steenkamp et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

et al. 2012

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“…The mating type locus conferring mating behaviour, consists of dissimilar DNA sequences (idiomorphs) in the mating partners of most ascomycetous fungi (Pöggeler 2001). Idiomorphs are highly dissimilar regions of DNA, which may contain from one to three open reading frames, in isolates of opposite mating type (Dyer et al 2001). In heterothallic species, mating type is controlled by a single locus with two idiomorphic alleles, designated MAT-1 and MAT-2 .…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Mating Type Genes in Asexually Reproducing Fusarium Oxysporum and F. Culmorum

Irzykowska¹,

Kosiada²

2011

Journal of Plant Protection Research

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Sexually (homothallic and heterothallic) and asexually reproducing species belong to the Fusarium genus. So far, there is no known sexual stage of the F. oxysporum Schlechtend.: Fr. and F. culmorum (W.G. Smith) Sacc. Knowing the reproduction mode is important for the design of successful control strategies, since they are different for clonally and sexually reproducing organisms. In examined sets of asexual F. oxysporum and F. culmorum isolates, the DNA sequences of mating type genes (idiomorphs MAT-1 and MAT-2) were identified. MAT-1 sequence was detected for 33 and 40% of F. oxysporum and F. culmorum isolates, respectively. For the remaining isolates a sequence specific for MAT-2 was amplified.

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“…For ex am ple Mycosphaerella graminicola, Phaeosphaeria nodorum, Rhynchosporium secalis and Tapesia yallundae or T.accuformis also have mixed pop u la tions struc ture clonal and re combin ing but ex hibit lit tle dif fer en ti a tion of pop u la tions (G ST < 0.05). In this case it has been found the low G ST val ues even be tween trans con ti nen tal pop u la tions (Douhan et al 2002, Dyer et al 2001, Salamati et al 2000, Zhan et al 2001. It is still de bated why some fun gal spe cies ex hibit sig nif i cantly greater dif fer en ti a tion among subpopulations.…”

Section: Discussionmentioning

confidence: 83%

Genotypic population structure of Ascochyta pinodes and Phoma pinodella in Poland

Furgał-Węgrzycka¹,

Węgrzycki²

2009

Plant Breeding and Seed Science

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The genotypic pop u la tions struc ture of Ascochyta pinodes and Phoma pinodella was de ter mined across a hi er ar chy of spa tial and tem po ral scale by us ing veg e ta tive in com pat i bil ity sys tem as phenotypic ge netic marker. Com po nents of ge netic vari a tion within and be tween pop u la tions in cluded the fol low ing val ues: in dices of di ver sity H', rich ness (g), in di ces of even ness E 1 , E 2 and E 5, spatio-tem po ral anal y sis of genotypic varia tion (H T and H S val ues), pairwise com par i sons of ge no type di ver sity, dis tri bu tion of VCGs across spatio-tem po ral scale (G ST value) and anal y sis of vari ance cal cu lated on VCGs ge no type fre quen cies within pop u la tions. It has been found that genotypic pop u la tions struc ture of Ascochyta pinodes and Phoma pinodella be long to mixed pop u la tion struc ture clonal and re com bin ing. Three re gional pop u la tions of Ascochyta pinodes and Phoma pinodella dif fered sig nif i cantly in both of VCGs ge no type fre quen cies and their dis tri bu tion on a spatio-tem po ral scale in di cat ing on the low or mod er ate de gree of ge no type mi gra tion be tween re gions of Po land.Keywords: Mycosphaerella (Didymella) pinodes anamorph Ascochyta pinodes, Phoma pinodella (teleomorph Didymella), genotypic pop u la tion struc ture, com po nents the ge netic vari a tion of pop u la tion, VCG fre quency, spa tial and tem po ral VCG diversity INTRODUCTIONAscochyta blight of pea is caused pri mar ily by Ascochyta pinodes (Berk et Blox) Jones teleomorph Mycosphaerella pinodes (Berk et Blox) Vesterg = Didymella pinodes, Pertrak and Phoma medicaginis var pinodella (Jones) Boerema = A.pinodella Jones (teleomorph Didymella). This dis ease provides a good ex am ple of the dif fi cul ties in seed in fec tions con trol ling and ep i dem ics of Ascochyta blight causes by patho gens that are highly host-spe cific and are ca pa ble of caus ing se ri ous dam age in a con duc tive envi ron ment. Since the area of pea has sys tem at i cally in creased in some countries of the world, Ascochyta blight of pea has be come one of the most im por tant dis eases in pea pro duc tion. Ex cept of di rect yield losses, Communicated by Ed ward ArseniukPLANT BREEDING AND SEED SCIENCE

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A Multiplex PCR Test for Determination of Mating Type Applied to the Plant Pathogens Tapesia yallundae and Tapesia acuformis

Cited by 51 publications

References 35 publications

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

Molecular Identification of Mating Type Genes in Asexually Reproducing Fusarium Oxysporum and F. Culmorum

Genotypic population structure of Ascochyta pinodes and Phoma pinodella in Poland

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