A multiplex RT-PCR method for the simultaneous detection ofNarcissus yellow stripe virus, Narcissus latent virusandNarcissus mosaic virus

First report of hippeastrum mosaic virus, narcissus late season yellows virus, narcissus latent virus and narcissus mosaic virus in daffodils from Hungary

“…For virus identification RNA was extracted from all collected samples and RT-PCR was carried out using universal potyvirus primers (Salamon and Palkovics 2005) and also with multiplex RT-PCR primers for detection of narcissus latent virus (NLV) and narcissus mosaic virus (NMV) (He et al 2019). From each daffodil sample similar sized PCR products were cloned into pGEM®-T Easy vector followed by nucleotide (nt) sequence determination, which resulted in identification of NLV (GenBank accession No.…”