A multiresidual method based on ion-exchange chromatography with conductivity detection for the determination of biogenic amines in food and beverages

Abstract

In the present work a sensitive and accurate method by ion chromatography and conductimetric detection has been developed for the determination of biogenic amines in food samples at microgram per kilogram levels. The optimized extraction procedure of trimethylamine, triethylamine, putrescine, cadaverine, histamine, agmatine, spermidine, and spermine from real samples, as well as the separation conditions based on a multilinear gradient elution with methanesulfonic acid and the use of a weak ionic exchange column, have provided excellent results in terms of resolution and separation efficiency. Extended calibration curves (up to 200 mg/kg, r > 0.9995) were obtained for all the analyzed compounds. The method gave detection limits in the range 23-65 μg/kg and quantification limits in spiked blank real samples in the range 65-198 μg/kg. Recovery values ranged from 82 to 103 %, and for all amines, a good repeatability was obtained with precision levels in the range 0.03-0.32 % (n = 4). The feasibility and potential of the method were tested by the analysis of real samples, such as tinned tuna fish, anchovies, cheese, wine, olives, and salami.

Determination of biogenic amines in oysters by capillary electrophoresis coupled with electrochemiluminescence

“…Among those were high performance liquid chromatography (HPLC) (Yen, & Hsieh, 1991;Li et al, 2014), capillary electrophoresis (CE) (Park et al, 2010), ion chromatography (IC) (Cinquina et al, 2004;Palermo, Muscarella, Nardiello, Iammarino, & Centonze, 2013), thin-layer chromatography (TLC) (Naguib, Ayesh, & Shalaby, 1995;Romano et al, 2012) and gas chromatography (GC) (Naila, Flint, Fletcher, Bremer, & Meerdink, 2011). HPLC requires extensive sample preparation, including derivatization of the amines to form non-polar volatile compounds (the highly polaramines tend to ''stick'' to the column and cause ''memory effects'') (Callejón, Sendra, Ferrer, & Pardo, 2013).…”

Biogenic Amines in Traditional Fiore Sardo PDO Sheep Cheese: Assessment, Validation and Application of an RP-HPLC-DAD-UV Method

“…Among others, liquid chromatography nowadays still represents the most preferred analytical choice [7,8,12,40,42,46,54,55], even sometimes coupled with a mass spectrometry detection [11,56,57]. Other chromatographic methods have also been used for this aim, like ion-exchange chromatography [51,58], ion chromatography [59] and gas-chromatography coupled with mass spectrometry [60]. Apart from chromatography, several analytical techniques can also be succesfully applied for measuring selected BAs: controlled-potential methods [22], enzyme-linked immunosorbent assay methods [61] and capillary electrophoresis techniques [62] have been proposed for the His determination, whereas amperometric biosensors were used for the determination of Tyr [63] and square wave adsorbpitive stripping voltammetry for the determination of Trp [64].…”

Developing and Validating a UPLC‐MS Method with a StageTip‐Based Extraction for the Biogenic Amines Analysis in Fish

“…Analysis of BAs is mainly based on chromatographic methods, including capillary electrophoresis (He, Xu, Hirokawa, & Shen, ), ion chromatography (Palermo, Muscarella, Nardiello, Iammarino, & Centonze, ), high‐performance liquid chromatography (HPLC; Manetta et al., ), gas chromatography‐mass spectrometry (Huang et al., ), and liquid chromatography‐mass spectrometry (LC‐MS; Nalazek‐Rudnicka & Wasik, ). Among these methods, analysis of the derivatized BAs via HPLC system coupled with UV detector is the most widely used (Papageorgiou et al., ).…”