2003
DOI: 10.1017/s1431927603445765
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A Multiresolution Autofocusing Method for Automated Microscopy

Abstract: Autofocusing is crucially important for automated microscopy, where fully automatic acquisition of microscope images in unattended operation is required. In addition to providing automation of imaging for scanning microscopy applications, it facilitates objective, accurate, and consistent image measurements for quantitative analysis. Autofocusing algorithms determine the in-focus position for an image based on maximization of a focus function, which represents the measure of focus as a function of the axial z … Show more

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Cited by 2 publications
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“…Multi-resolution wavelet approaches suggest a very suitable means for highly efficient autofocuing. Our results show that wavelet based approach overcomes the fundamental limitations of existing single resolution autofocusing techniques and yield superior performance [11].…”
Section: Optimization Of Optical Microscope System Performance For Ac...mentioning
confidence: 80%
“…Multi-resolution wavelet approaches suggest a very suitable means for highly efficient autofocuing. Our results show that wavelet based approach overcomes the fundamental limitations of existing single resolution autofocusing techniques and yield superior performance [11].…”
Section: Optimization Of Optical Microscope System Performance For Ac...mentioning
confidence: 80%