A multiscale approach to assess thermomechanical performance and force generation in nanorobotic microgels

Wang, Chen; Harder, Philipp; İyisan, Nergishan; Li, Bolin; Hiendlmeier, Lukas; Wolfrum, Bernhard; Özkale, Berna

doi:10.1039/d3nr06485a

Nanoscale

2024

DOI: 10.1039/d3nr06485a

|View full text |Cite

A multiscale approach to assess thermomechanical performance and force generation in nanorobotic microgels

Chen Wang,

Philipp Harder,

Nergishan İyisan

et al.

Abstract: We present a multiscale approach to characterize the performance of photothermally powered, nanorobotic 3D microgels. Optically triggered nanoactuators, consisting of a gold nanorod core and thermoresponsive pNIPMAM shell, are used...

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Mechanoactivation of Single Stem Cells in Microgels Using a 3D‐Printed Stimulation Device

İyisan,

Hausdörfer,

Wang

et al. 2024

Small Methods

View full text Add to dashboard Cite

In this study, the novel 3D‐printed pressure chamber for encapsulated single‐cell stimulation (3D‐PRESS) platform is introduced for the mechanical stimulation of single stem cells in 3D microgels. The custom‐designed 3D‐PRESS, allows precise pressure application up to 400 kPa at the single‐cell level. Microfluidics is employed to encapsulate single mesenchymal stem cells within ionically cross‐linked alginate microgels with cell adhesion RGD peptides. Rigorous testing affirms the leak‐proof performance of the 3D‐PRESS device up to 400 kPa, which is fully biocompatible. 3D‐PRESS is implemented on mesenchymal stem cells for mechanotransduction studies, by specifically targeting intracellular calcium signaling and the nuclear translocation of a mechanically sensitive transcription factor. Applying 200 kPa pressure on individually encapsulated stem cells reveals heightened calcium signaling in 3D microgels compared to conventional 2D culture. Similarly, Yes‐associated protein (YAP) translocation into the nucleus occurs at 200 kPa in 3D microgels with cell‐binding RGD peptides unveiling the involvement of integrin‐mediated mechanotransduction in singly encapsulated stem cells in 3D microgels. Combining live‐cell imaging with precise mechanical control, the 3D‐PRESS platform emerges as a versatile tool for exploring cellular responses to pressure stimuli, applicable to various cell types, providing novel insights into single‐cell mechanobiology.

show abstract

Mechanoactivation of Single Stem Cells in Microgels Using a 3D‐Printed Stimulation Device

İyisan,

Hausdörfer,

Wang

et al. 2024

Small Methods

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A multiscale approach to assess thermomechanical performance and force generation in nanorobotic microgels

Abstract: We present a multiscale approach to characterize the performance of photothermally powered, nanorobotic 3D microgels. Optically triggered nanoactuators, consisting of a gold nanorod core and thermoresponsive pNIPMAM shell, are used...

Cited by 1 publication

References 44 publications

Mechanoactivation of Single Stem Cells in Microgels Using a 3D‐Printed Stimulation Device

Mechanoactivation of Single Stem Cells in Microgels Using a 3D‐Printed Stimulation Device

Contact Info

Product

Resources

About