A Multiscale Model of Fungal Impact on Chemotactic Behavior of Mycorrhizal Helper Bacteria

Britton, Jolene; Ramezani, Alireza; Pelletier, Dale A.; Alber, Mark; Cannon, W. Roger

doi:10.1016/j.bpj.2020.11.639

Cited by 3 publications

(4 citation statements)

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“…Horizontal branch lengths are proportional to the scale of the substitutions. The promotion of mycorrhizal fungi by MHB is the result of a combination of several aspects, including the fact that MHB can produce a variety of metabolites to promote the growth of mycorrhizal fungi, which is also one of the most important roles of MHB (Britton et al, 2021). Therefore, mycelial growth after mycorrhizal fungalbacterial isolate interactions also becomes an important indicator for the rapid screening of MHB.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of mycorrhizal helper bacteria of Vaccinium uliginosum and their interaction with mycorrhizal fungi

et al. 2023

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Mycorrhizal helper bacteria (MHB) can promote mycorrhizal fungal colonization and form mycorrhizal symbiosis structures. To investigate the effect of interactions between mycorrhizal beneficial microorganisms on the growth of blueberry, 45 strains of bacteria isolated from the rhizosphere soil of Vaccinium uliginosum were screened for potential MHB strains using the dry-plate confrontation assay and the bacterial extracellular metabolite promotion method. The results showed that the growth rate of mycelium of Oidiodendron maius 143, an ericoid mycorrhizal fungal strain, was increased by 33.33 and 77.77% for bacterial strains L6 and LM3, respectively, compared with the control in the dry-plate confrontation assay. In addition, the extracellular metabolites of L6 and LM3 significantly promoted the growth of O. maius 143 mycelium with an average growth rate of 40.9 and 57.1%, respectively, the cell wall-degrading enzyme activities and genes of O. maius 143 was significantly increased. Therefore, L6 and LM3 were preliminarily identified as potential MHB strains. In addition, the co-inoculated treatments significantly increased blueberry growth; increased the nitrate reductase, glutamate dehydrogenase, glutamine synthetase, and glutamate synthase activities in the leaves; and promoted nutrient uptake in blueberry. Based on the physiological, and 16S rDNA gene molecular analyses, we initially identified strain L6 as Paenarthrobacter nicotinovorans and LM3 as Bacillus circulans. Metabolomic analysis revealed that mycelial exudates contain large amounts of sugars, organic acids and amino acids, which can be used as substrates to stimulate the growth of MHB. In conclusion, L6 and LM3 and O. maius 143 promote each other’s growth, while co-inoculation of L6 and LM3 with O. maius 143 can promote the growth of blueberry seedlings, providing a theoretical basis for further studies on the mechanism of ericoid mycorrhizal fungi-MHB-blueberry interactions. It laid the technical foundation for the exploitation of biocontrol strain resources and the development of biological fertilizer.

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Section: Discussionmentioning

confidence: 99%

Isolation and identification of mycorrhizal helper bacteria of Vaccinium uliginosum and their interaction with mycorrhizal fungi

et al. 2023

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“…The nutritional requirements of bacteria and fungi play a role in the interaction. The mycorrhizal fungi Laccaria bicolor synthesize trehalose, which stimulates the growth and chemotaxis of the MHB Psuedomonas fluorescens and acquires thiamine from p. flourescens [ 37 ]. Furthermore, MHB have the ability to receive nourishment from the outer hyaline spore layer, and these bacteria were found to possess the ability to produce cell-wall-degrading enzymes, including cellulase, chitinase, and protease, and are capable of breaking down exopolysaccharides, which may lead to their increase in relative abondance [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Responses of Bacterial Community Structure, Diversity, and Chemical Properties in the Rhizosphere Soil on Fruiting-Body Formation of Suillus luteus

et al. 2022

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Mycorrhiza helper bacteria (MHB) play an important role in driving mycorrhizal formation. There are few reports on the relationship between bacteria and fruiting growths. Taking mycorrhizal rhizosphere soil from sporocarps of the S. luteus and non-mycorrhizal rhizosphere soil of the host plant (Larix gmelinii), we measured the bacterial community structure and diversity and chemical properties to clarify the effect of bacteria on fruiting-body formation. The bacterial diversity was significantly higher in mycorrhizal rhizosphere soil (p < 0.05) than that in non-mycorrhizal rhizosphere soil. The relative abundance of Burkholderia, Bradyrhizobium, Pseudomonas, and Rhizobium was significantly higher (p < 0.05) in mycorrhizal rhizosphere soil than in non-mycorrhizal rhizosphere soil. The soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), ammonium nitrogen (AN), available phosphorus (AP), available potassium (AK), and the activity of catalase, urease, and phosphatase in mycorrhizal rhizosphere soil were significantly higher (p < 0.05) than those in non-mycorrhizal rhizosphere soil. A redundancy analysis (RDA) showed that dominant bacteria are closely related to soil enzyme activity and physicochemical properties (p < 0.05). The boletus recruits a large number of bacteria around the plant roots that speed up nutrient transformation and increase the soil nutrient content, providing an important guarantee for mycelium culture and fruiting-body formation. These findings provide ideas for the nutritional supply of boletus sporocarps and lay the theoretical foundation for the efficient artificial cultivation of boletus.

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“…Efek menguntungkan pada tanaman dari fitohormon yang dikeluarkan oleh FMA dan MHB diperlihatkan dari adanya peningkatan pembentukan akar dan tidak adanya efek menghambat atau menekan. Hal ini menunjukkan adanya pengaruh fungi terhadap komponen biokimia yang dihasilkan MHB (Britton et al, 2021). Perlakuan FMA, MHB maupun kombinasi dari FMA dan MHB terhadap pertumbuhan akar maupun planlet kentang secara keseluruhan memberikan efek yang positif.…”

Section: Rancangan Percobaanunclassified

“…Pemanfaatan FMA maupun MHB pada tanaman kentang terus mengalami perkembangan (Lallawmkima et al, 2018), akan tetapi kajian maupun informasi mengenai mekanisme interaksi kedua mikroba tersebut, khususnya FMA dan MHB pada stek kentang in vitro dalam kultur jaringan masih sangat terbatas. Hasil penelitian pada tanaman kentang menunjukkan bahwa efektivitas FMA meningkat jika dikombinasikan dengan MHB Pseudomonas diminuta (Putri et al, 2020;Britton et al, 2021;Wang et al, 2022b). Fungi mikoriza arbuskula mengeluarkan berbagai jenis eksudat seperti formiat, asetat, beta glikogen yang dapat memacu pertumbuhan tanaman dan komunitas bakteri fungsional (Toljander et al, 2007;Fritz et al, 2022).…”

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Kandungan Hormon dan Pertumbuhan Tanaman pada Bioassay Bibit Kentang yang Diberi Fungi Mikoriza Arbuskula dan Mycorrhizal Helper Bacteria

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2024

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Produksi bibit tanaman kentang secara in vitro melalui kultur jaringan merupakan teknik yang sedang berkembang. Berbagai upaya peningkatan kualitas bibit tanaman kentang telah dilakukan dan salah satu alternatifnya adalah dengan aplikasi pupuk hayati fungi mikoriza arbuskula (FMA) dan mycorrhizal helper bacteria (MHB). Penelitian ini bertujuan untuk mengevaluasi respons pertumbuhan serta hormon yang dihasilkan FMA dan MHB pada bibit tanaman kentang yang dikulturkan secara in vitro. Percobaan menggunakan Rancangan Acak Lengkap yang terdiri atas empat jenis media tumbuh dan sepuluh replikasi. Media tumbuh meliputi Murashige & Skoog (MS) kontrol, dengan FMA, MHB, dan kombinasi FMA dan MHB. Untuk melakukan inokulasi, 10 spora FMA (Glomus sp.) yang telah disterilisasi ditransfer ke zona akar tanaman, dan MHB (Pseudomonas diminuta) ditambahkan ke dalam media. Pengamatan dilakukan terhadap pertumbuhan bibit, berat segar akar dan pupus, serta hormon auksin, giberelin dan sitokinin (2 MST). Hasil penelitian menunjukkan bahwa FMA dan MHB menghasilkan hormon auksin, giberelin dan sitokinin. Efek pupuk hayati terhadap hormon tumbuh yang dihasilkan dan pertumbuhan bibit kentang berbeda secara signifikan. Selanjutnya, inokulasi FMA tunggal memiliki pengaruh yang sama dengan media MSR yang diinokulasi kombinasi FMA dan MHB terhadap pertumbuhan bibit tanaman kentang. Sintesis hormon sangat signifikan pada media yang diinokulasi MHB. Hasil penelitian ini menunjukkan bahwa aplikasi pupuk hayati FMA bersama MHB mempengaruhi pertumbuhan tanaman yaitu tinggi tanaman sebesar 9,01 cm atau 19,6% lebih tinggi dibandingkan dengan kontrol dan meningkatkan bobot segar sebesar 12,72 g atau 60% lebih tinggi dibandingkan kontrol. Peningkatan pertumbuhan tanaman ini terkait dengan tingkat hormon tumbuh yang dihasilkan oleh FMA dan MHB yang juga lebih tinggi yaitu 4,06, 8,62 dan 5,77 µmol/mol NTP berturut-turut untuk auksin, giberelin dan sitokinin.

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A Multiscale Model of Fungal Impact on Chemotactic Behavior of Mycorrhizal Helper Bacteria

Cited by 3 publications

References 0 publications

Isolation and identification of mycorrhizal helper bacteria of Vaccinium uliginosum and their interaction with mycorrhizal fungi

Isolation and identification of mycorrhizal helper bacteria of Vaccinium uliginosum and their interaction with mycorrhizal fungi

Responses of Bacterial Community Structure, Diversity, and Chemical Properties in the Rhizosphere Soil on Fruiting-Body Formation of Suillus luteus

Kandungan Hormon dan Pertumbuhan Tanaman pada Bioassay Bibit Kentang yang Diberi Fungi Mikoriza Arbuskula dan Mycorrhizal Helper Bacteria

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