A multispot DNA chip fabricated with mixed ssDNA/oligo (ethylene glycol) self-assembled monolayers for detecting the effect of secondary structures on hybridization by SPR imaging

Chen, W. Y.; Hu, Wen-Pin; Su, Yuan Deng; Taylor, Allen D.; Jiang, Shaoyi; Chang, Guan‐Liang

doi:10.1016/j.snb.2007.03.006

Cited by 17 publications

(16 citation statements)

References 38 publications

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“…[20,21] Along this line of thought we fabricated a dual pH/light-reversible surface for the binding of biomolecules. [20,21] Along this line of thought we fabricated a dual pH/light-reversible surface for the binding of biomolecules.…”

Section: Preparation and Characterization Of Ph/light-sensitive Surfacesmentioning

confidence: 99%

“…Recent studies have focused on the development of new approaches towards binding biomolecules to a surface in an easy and controllable way. [20,21] Along this line of thought we fabricated a dual pH/light-reversible surface for the binding of biomolecules. Our approach, using an azobenzene-modified surface to which DNA molecules can be attached, provides an irreversible and controllable DNA sensor platform.…”

Section: Preparation and Characterization Of Ph/light-sensitive Surfacesmentioning

confidence: 99%

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A Novel pH/Light‐Triggered Surface for DNA Adsorption and Release

Demirel¹

2014

ChemPhysChem

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A simple strategy for the immobilization of Cy3-labeled single strand DNA (Cy3-ssDNA) on a Si(001) surface and its release under control of both light and pH stimuli is presented. In order to prepare a dual pH/light-triggered surface, positively chargeable azobenzene molecules are self-assembled on the Si(001) surface. The surface wettability of this substrate can be changed under influence of both light and pH conditions. The substrates can be positively charged under mildly acidic conditions. The pH-sensitive behavior of the film allows binding of Cy3-ssDNA on the functionalized Si(001) surface through eﬀective electrostatic interactions with the negatively charged polynucleotide backbone. Moreover, irradiation of the film with UVA light induces trans-cis isomerization of the azobenzene units on the surface. As a result, the binding aﬃnity for DNA decreases due to the changing surface hydrophilicity. In order to understand and control the reversible photoswitchable mechanism of this surface, water contact angles are measured after UVA and visible light irradiation. The release of DNA from a dual pH/light-sensitive sample is performed using fluorescence microscopy. The results show that irradiation of the film with UVA light induces trans-cis isomerization of the photoresponsive azobenzene units; this leads to significant changes in the surface hydrophilicity and reduces the binding affinity for DNA.

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Section: Preparation and Characterization Of Ph/light-sensitive Surfacesmentioning

confidence: 99%

Section: Preparation and Characterization Of Ph/light-sensitive Surfacesmentioning

confidence: 99%

A Novel pH/Light‐Triggered Surface for DNA Adsorption and Release

Demirel¹

2014

ChemPhysChem

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“…The thiol-labeled capture probe (C1) was immobilized and a gold-S (from thiol) covalent linkage was formed on a gold surface [5,6,8,9]. Target probes (T1 and T1-dye) were designed with a sectional sequence of avian influenza A virus (A/avian/NY/73-63-6/00(H7N2)) hemagglutinin.…”

Section: Preparation Of Reagents and Probesmentioning

confidence: 99%

“…Among various SPR measurement schemes, SPR imaging systems have been explored extensively due to their potential for use in high-throughput sensing applications [3,5,6]. An intensity-based SPR imaging system comprising a monochromatic light source and a fixed incidence angle has a simple structure.…”

mentioning

confidence: 99%

Detection of Avian Influenza-DNA Hybridization Using Wavelength-scanning Surface Plasmon Resonance Biosensor

Kim¹,

Kim²,

Park³

et al. 2009

Journal of the Optical Society of Korea

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“…[4,5] Depending on the particular application, immobilization can be performed either homogeneously over the entire surface or lithographically, resulting in an array of ssDNA spots. [4,[7][8][9] The hybridization activity of supported ssDNA depends on the packing density and molecular organization, which can be manipulated by diluting ssDNA with other molecules that have the same reactive group. [4,[10][11][12][13] For a gold substrate, the diluent molecules of choice are short-chain alkanethiols (ATs) [4,[12][13][14][15][16] or thiolated oligo(ethylene glycol)s (OEG-ATs).…”

mentioning

confidence: 99%

Fabrication of ssDNA/Oligo(ethylene glycol) Monolayers and Complex Nanostructures by an Irradiation‐Promoted Exchange Reaction

et al. 2012

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Creative design: An approach to preparing mixed monolayers of thiolated single‐stranded DNA (ssDNA) and oligo(ethylene glycol)s (OEG‐AT) in a broad range of compositions as well as ssDNA/OEG‐AT patterns of any required shape (see top figure) has been shown. A combination of this approach with surface‐initiated enzymatic polymerization allows complex 3D DNA nanostructures to be sculpted with high spatial precision (bottom).

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A multispot DNA chip fabricated with mixed ssDNA/oligo (ethylene glycol) self-assembled monolayers for detecting the effect of secondary structures on hybridization by SPR imaging

Cited by 17 publications

References 38 publications

A Novel pH/Light‐Triggered Surface for DNA Adsorption and Release

A Novel pH/Light‐Triggered Surface for DNA Adsorption and Release

Detection of Avian Influenza-DNA Hybridization Using Wavelength-scanning Surface Plasmon Resonance Biosensor

Fabrication of ssDNA/Oligo(ethylene glycol) Monolayers and Complex Nanostructures by an Irradiation‐Promoted Exchange Reaction

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