A Multistage Process to Enhance Cellobiose Production from Cellulosic Materials

Vanderghem, Caroline; Boquel, Pascal; Blecker, Christophe; Paquot, Michel

doi:10.1007/s12010-009-8724-7

Cited by 27 publications

(19 citation statements)

References 23 publications

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“…Usually, b-glucosidase in the cellulase complex is responsible for the conversion of cellobiose into glucose. It is possible to control cellulose hydrolysis to accumulate more cellobiose over glucose (Homma et al, 1993;Vanderghem et al, 2009;Kim and Day, 2010), and production of ethanol from cellobiose has been demonstrated using yeast strains heterologously expressing b-glucosidase (Vanrooyen et al, 2005;Gurgu et al, 2011). Also, cellobiose and xylose co-fermentation has been used to produce ethanol (Nakamura et al, 2008;Saitoh et al, 2010;Li et al, 2010;Ha et al, 2011).…”

Section: Lipid Production By L Starkeyi On Cellobiose Glucose or Xymentioning

confidence: 99%

Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production

Gong

Wang

Shen

et al. 2012

Bioresource Technology

160

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Section: Lipid Production By L Starkeyi On Cellobiose Glucose or Xymentioning

confidence: 99%

Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production

Gong

Wang

Shen

et al. 2012

Bioresource Technology

160

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“…The multistage hydrolysis (four times, 6 h) for both celluloses was performed according to the procedure of Vanderghem et al [11]. Celluloses were suspended at a concentration of 50 g/L in citrate buffer.…”

Section: Water Retention Valuementioning

confidence: 99%

“…In a previous study, cellulose enzymatic hydrolysis was investigated in a multistage process to enhance the cellobiose production of a cellulose fiber [11]. In this study, two more types of cellulose, varying in crystallinity and particle size (microcrystalline cellulose and paper pulp), were evaluated for the production of cellobiose under the multistage process.…”

Section: Introductionmentioning

confidence: 99%

Effect of Physicochemical Characteristics of Cellulosic Substrates on Enzymatic Hydrolysis by Means of a Multi-Stage Process for Cellobiose Production

Vanderghem

Jacquet

Danthine

et al. 2012

Appl Biochem Biotechnol

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The effect of two types of cellulose, microcrystalline cellulose and paper pulp, on enzymatic hydrolysis for cellobiose production was investigated. The particle size, the relative crystallinity index and the water retention value were determined for both celluloses. A previously studied multistage hydrolysis process that proved to enhance the cellobiose production was studied with both types of celluloses. The cellobiose yield exhibited a significant improvement (120% for the microcrystalline cellulose and 75% for the paper pulp) with the multistage hydrolysis process compared to continuous hydrolysis. The conversion of cellulose to cellobiose was greater for the microcrystalline cellulose than for the paper pulp. Even with high crystallinity, microcrystalline cellulose achieved the highest cellobiose yield probably due to its highest specific surface area accessible to enzymes and quantity of adsorbed protein.

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“…The practical amylose yield could be enhanced through more cellobiose production by optimizing the cellulase mixture composition and ratio, eliminating beta-glucosidase from the commercial cellulase mixtures, optimization of CBP-PGP loading, improving PGP performance, process design (47), and biomass pretreatment conditions. For potential application, the current amylose yields (e.g., 2-30%) in biorefineries would be acceptable because no sugar is wasted and the potential market for synthetic starch as food and feed could be less than 1/10th that of biofuels and biochemicals (4,48).…”

Section: Discussionmentioning

confidence: 99%

Enzymatic transformation of nonfood biomass to starch

You¹,

Chen

Myung³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

149

123

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The global demand for food could double in another 40 y owing to growth in the population and food consumption per capita. To meet the world's future food and sustainability needs for biofuels and renewable materials, the production of starch-rich cereals and cellulose-rich bioenergy plants must grow substantially while minimizing agriculture's environmental footprint and conserving biodiversity. Here we demonstrate one-pot enzymatic conversion of pretreated biomass to starch through a nonnatural synthetic enzymatic pathway composed of endoglucanase, cellobiohydrolyase, cellobiose phosphorylase, and alpha-glucan phosphorylase originating from bacterial, fungal, and plant sources. A special polypeptide cap in potato alpha-glucan phosphorylase was essential to push a partially hydrolyzed intermediate of cellulose forward to the synthesis of amylose. Up to 30% of the anhydroglucose units in cellulose were converted to starch; the remaining cellulose was hydrolyzed to glucose suitable for ethanol production by yeast in the same bioreactor. Next-generation biorefineries based on simultaneous enzymatic biotransformation and microbial fermentation could address the food, biofuels, and environment trilemma.bioeconomy | food and feed | synthetic amylose | in vitro synthetic biology | cell-free biomanufacturing T he continuing growth of the population and food consumption per capita means that the global demand for food could increase by 50-100% by 2050 (1, 2), and ∼30% of the world's agricultural land and 70% of the world's fresh water withdrawals are being used for the production of food and feed to support 7 billion people (3, 4). Starch is the most important dietary component because it accounts for more than half of the consumed carbohydrates, which provide 50-60% of the calories needed by humans. Starch is composed of polysaccharides consisting of a large number of glucose units joined together primarily by alpha-1,4-glycosidic bonds and alpha-1,6-glycosidic bonds. Linear-chain amylose is more valuable than branched amylopectin because it can be used as a precursor for making high-quality transparent, flexible, low-oxygen-diffusion plastic sheets and films (5, 6); tailored functional food or additives for lowering the risk of serious noninfectious diseases (e.g., diabetes and obesity) (7, 8); and a potential high-density hydrogen carrier (9-11). Also, it is easy to convert linear amylose to branched amylopectin by using alphaglucan-branching glycosyltransferase (12).Cellulose, a linear glucan linked by beta-1,4-glycosidic bonds, is the supporting material of plant cell walls and the most abundant carbohydrate on Earth. The annual resource of cellulosic materials is ∼40 times greater than the starch produced by crops cultivated for food and feed. In addition, (perennial) cellulosic plants and dedicated bioenergy crops can grow on low-quality land, even on marginal land, and require fewer inputs such as fertilizers, herbicides, pesticides, and water, whereas annual high-productivity starch-rich crops require high-qual...

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A Multistage Process to Enhance Cellobiose Production from Cellulosic Materials

Cited by 27 publications

References 23 publications

Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production

Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production

Effect of Physicochemical Characteristics of Cellulosic Substrates on Enzymatic Hydrolysis by Means of a Multi-Stage Process for Cellobiose Production

Enzymatic transformation of nonfood biomass to starch

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