A multivariate generalized linear model of the effect of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase-1 in end-stage renal disease

Abstract

End-stage renal disease (ESRD) is a serious disease that requires hemodialysis as the main regimen for supporting patients. The pathophysiology of the disease is still an interesting field of study. In the present study, matrix metalloproteinase-3 (MMP3), a tissue inhibitor of metalloproteinase-1 (TIMP1), and vitamin D (VitD) were studied in ESRD. MMP3, TIMP1, VitD, and other related biomarkers were measured in 53 ESRD patients and compared with 33 healthy controls. The results showed a significant increase in serum MMP3 and TIMP1 and a significant decrease in serum VitD in ESRD patients compared with the control group. The multivariate GLM results revealed no significant effects of the covariates (sex, age, smoking and BMI) on the levels of MMP3, TIMP1 and VitD. ESRD was found to be the major factor affecting the measured parameters, with a huge effect size (Partial η2) of 0.824. According to our data, eGFR, Creatinine, Urea, phosphate, uric acid and VitD (as estimated glomerular filtration rate) are the top 6 factors highly affected by the presence of the disease. MMP3 and TIMP1 are important parameters in ESRD and may act as drug targets. As the patients also suffered from hypovitaminosis D and hypocalcemia, these factors also need intervention.