1997
DOI: 10.1016/s0264-410x(96)00227-7
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A murine model of intranasal immunization to assess the immunogenicity of attenuated Salmonella typhi live vector vaccines in stimulating serum antibody responses to expressed foreign antigens

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Cited by 101 publications
(100 citation statements)
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“…Although wild-type or attenuated S. typhi are restricted to human host, intranasal administration of Ty21a to mice can lead to bacterial infection and persistence in the lung, 14 without deeper invasion to the spleen. 15 It is therefore important to examine the fate of Ty21a bacteria after intravesical instillation.…”
Section: Ty21a Bacteria Do Not Persist In Mice Tissues or Human Cellsmentioning
confidence: 99%
“…Although wild-type or attenuated S. typhi are restricted to human host, intranasal administration of Ty21a to mice can lead to bacterial infection and persistence in the lung, 14 without deeper invasion to the spleen. 15 It is therefore important to examine the fate of Ty21a bacteria after intravesical instillation.…”
Section: Ty21a Bacteria Do Not Persist In Mice Tissues or Human Cellsmentioning
confidence: 99%
“…immunization route, borrowing from the success of i.n. administration of serovar Typhi in mice and of Shigella in guinea pigs (4,5,11,22,(34)(35)(36). By careful titration, a dosage level (10 9 CFU) and an inoculum volume (20 l) were identified that were well tolerated and elicited immune responses to the live vector strain.…”
Section: Discussionmentioning
confidence: 99%
“…Serovar Typhi strain CVD 908-htrA, a ⌬aroC ⌬aroD ⌬htrA mutant (47), and the derivatives CVD 908-htrA(pTETlpp) (expressing tetanus toxin [TT] fragment C under control of a powerful constitutive prokaryotic promoter) (11,36,37,46), CVD 908-htrA(pGA3), CVD 908-htrA(pGA3-mH), CVD 908-htrA(pSINCP), and CVD 908-htrA(pMSIN-H) were streaked from a glycerol stock onto 2ϫ LB agar plates containing 20 g of Bacto tryptone/liter, 10 g of Bacto yeast extract (Difco, Detroit, Mich.)/liter, and 50 mM NaCl (Sigma) supplemented with 0.0001% (wt/vol) 2,3-dihydroxybenzoic acid (DHB; Sigma) and kanamycin (40 g/ml), where necessary. Colonies were suspended in LB broth supplemented with DHB and kanamycin and grown overnight.…”
Section: Methodsmentioning
confidence: 99%
“…Salmonella serovar Typhi strains used in this work were grown in media supplemented with 2,3-dihydroxybenzoic acid (DHB) (Sigma, St. Louis, Mo.) (14,19). Plasmidbearing strains of CVD 908-htrA were streaked from frozen (Ϫ70°C) master stocks on 2ϫ Luria-Bertani agar (solid medium) containing 20 g of Bacto Tryptone, 10 g of Bacto Yeast Extract, and 50 mM NaCl (2ϫLB50 agar) plus kanamycin at 10 mg/ml or carbenicillin at 50 mg/ml, as appropriate.…”
Section: Methodsmentioning
confidence: 99%
“…Unless otherwise noted, native Taq DNA polymerase (Invitrogen Life Technologies) was used in PCRs. Salmonella serovar Typhi strains were prepared for electroporation of recombinant plasmids after being harvested from 2ϫLB50 broth supplemented with DHB, and electroporation was carried out as previously described (14). Isolated colonies were swabbed on supplemented 2ϫLB50 agar and incubated at 30°C for 16 h. Frozen master stocks were prepared by harvesting bacteria into SOC medium (Invitrogen Life Technologies) without further supplementation and freezing at Ϫ70°C.…”
Section: Methodsmentioning
confidence: 99%