A Murine Model of Latex Allergy-Induced Airway Hyperreactivity

Thakker, J. C.; Xia, Jinqi; Rickaby, D. A.; Krenz, Gary S.; Kelly, Kevin J.; Kurup, Viswanath P.; Dawson, Christopher A.

doi:10.1007/pl00007633

Cited by 23 publications

(15 citation statements)

References 33 publications

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“…Intranasal challenge with latex allergens induced a strong IgE and eosinophil response with characteristic inflammatory changes in the lungs of mice as reported previously [21,22]. Other features of this model included enhanced IL-4 secretion by antigen stimulated spleen cells and reduced production of IFN-γ.…”

Section: Discussionsupporting

confidence: 71%

Immune response modulation by curcumin in a latex allergy model

et al. 2007

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BackgroundThere has been a worldwide increase in allergy and asthma over the last few decades, particularly in industrially developed nations. This resulted in a renewed interest to understand the pathogenesis of allergy in recent years. The progress made in the pathogenesis of allergic disease has led to the exploration of novel alternative therapies, which include herbal medicines as well. Curcumin, present in turmeric, a frequently used spice in Asia has been shown to have anti-allergic and inflammatory potential.MethodsWe used a murine model of latex allergy to investigate the role of curcumin as an immunomodulator. BALB/c mice were exposed to latex allergens and developed latex allergy with a Th2 type of immune response. These animals were treated with curcumin and the immunological and inflammatory responses were evaluated.ResultsAnimals exposed to latex showed enhanced serum IgE, latex specific IgG1, IL-4, IL-5, IL-13, eosinophils and inflammation in the lungs. Intragastric treatment of latex-sensitized mice with curcumin demonstrated a diminished Th2 response with a concurrent reduction in lung inflammation. Eosinophilia in curcumin-treated mice was markedly reduced, co-stimulatory molecule expression (CD80, CD86, and OX40L) on antigen-presenting cells was decreased, and expression of MMP-9, OAT, and TSLP genes was also attenuated.ConclusionThese results suggest that curcumin has potential therapeutic value for controlling allergic responses resulting from exposure to allergens.

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Section: Discussionsupporting

confidence: 71%

Immune response modulation by curcumin in a latex allergy model

et al. 2007

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“…The effect of sensitization on C dyn , if real, was too subtle to detect a correlation between C dyn and the magnitude of the inflammatory response. When challenged, C dyn was significantly reduced in all groups, perhaps due to increased parenchymal stress in the radial direction around contracted airways [27]. C dyn was significantly lower in sensitized mice compared to the challenged unsensitized mice, where R L was significantly increased.…”

Section: Discussionmentioning

confidence: 99%

“…Airway reactivity was assessed using a methacholine challenge test as previously described [9, 26, 27]. Briefly, each mouse was anesthetized with a solution containing ketamine and xylazine (40 mg/kg each, i.p.…”

Section: Methodsmentioning

confidence: 99%

Alkaline Serine Proteinase from <i>Aspergillus fumigatus</i> Has Synergistic Effects on Asp-f-2-Induced Immune Response in Mice

Kurup

Xia

Shen

et al. 2002

Int Arch Allergy Immunol

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Background: Exposure to Aspergillus fumigatus allergens results in the sensitization and the development of allergic bronchopulmonary aspergillosis in susceptible individuals. Aspergillus antigen consists of a number of chemically diverse components and their cumulative or synergistic effect may result in disease. When mice were challenged with individual recombinant allergens, there was only reduced inflammation and immunological responses compared to the whole antigen. Various enzymes identified from A. fumigatus have been thought to cause airway damage. In the present study, we evaluated the effect of exposure to Asp f 13, an alkaline serine proteinase, and Asp f 2 in mice. Methods: BALB/c mice were challenged intranasally with Asp f 2 and Asp f 13 alone and in combination. The antibody response, pulmonary inflammation, and airway hyperreactivity were studied. Results: Results demonstrated no major difference in antibody response and airway responses among the different groups. The inflammatory responses in the lungs, however, showed marked differences in the various groups. Conclusion: In spite of the similar immunological responses in the different groups of mice studied, the results demonstrate enhanced inflammation in the lungs of mice exposed to a combination of both allergens. Allergens with proteinase activity have been found to be involved in airway inflammation and remodeling, which may also apply for Aspergillus-induced allergy.

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“…Animals were anesthetized and tracheotomized and lung mechanics were evaluated using a whole-body Plexiglas plethysmograph chamber as described previously [23, 29]. …”

Section: Methodsmentioning

confidence: 99%

Gastro-Intestinal Exposure to Latex Antigens Induce Allergic Responses in Mice

Barrios

Kurup

Rickaby

et al. 2006

Int Arch Allergy Immunol

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Background: Natural rubber latex (NRL) has emerged as a major cause of respiratory allergy among specific exposed groups of individuals. Since latex allergens are dispersed in the environment it is conceivable that latex proteins are both inhaled and ingested. The mechanism of latex allergy and the immune responses following reexposure of latex allergens by the intranasal route was studied in a murine model of latex allergy developed by intragastric sensitization with NRL. Methods:BALB/c mice were sensitized intragastrically (‘ig’), intranasally (‘in’) or ‘ig’ followed by ‘in’ challenge with NRL allergens. The cellular and humoral immune responses, lung function and histological changes were determined. Results: Peripheral blood eosinophilia was observed in the ‘ig’ and ‘ig/in’-NRL-sensitized animals in comparison to normal controls (p < 0.05). The ‘ig’ group showed a marked increase over control mice in serum total IgE, NRL-specific IgG and IgG subclasses (p < 0.05). Increased levels of IL-4, IL-5, IL-10, and IL-13 were detected in ‘ig’-NRL-sensitized mice. Intranasal exposure with NRL after ‘ig’ sensitization further enhanced the cytokine levels. A tendency towards enhanced stimulation was determined in ‘ig’-sensitized mice; a significant difference was shown in the ‘ig/in’-group (p < 0.05). Increased airway hyperreactivity was found in ‘ig’-NRL-sensitized-mice (15.1 ± 2.5 vs. 8.9 ± 1.7 cm H₂O·ml^–1·s, p < 0.05). Mucus secretion from jejunal epithelium and eosinophilic infiltration into the jejunal lamina propria were observed in the ‘ig’-NRL-sensitized-mice. Conclusions:The results demonstrate that intragastric NRL sensitization did not induce specific tolerance, and additional intranasal exposure with latex allergens resulted in systemic allergic manifestations in the murine model.

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A Murine Model of Latex Allergy-Induced Airway Hyperreactivity

Cited by 23 publications

References 33 publications

Immune response modulation by curcumin in a latex allergy model

Immune response modulation by curcumin in a latex allergy model

Alkaline Serine Proteinase from <i>Aspergillus fumigatus</i> Has Synergistic Effects on Asp-f-2-Induced Immune Response in Mice

Gastro-Intestinal Exposure to Latex Antigens Induce Allergic Responses in Mice

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