A murine model of thoracic aortic aneurysms

Ikonomidis, John S.; Gibson, William C.; Gardner, Jonathan; Sweterlitsch, Sarah E.; Thompson, Robert P.; Mukherjee, Rupak; Spinale, Francis G.

doi:10.1016/s0022-4804(03)00193-8

Cited by 68 publications

(78 citation statements)

References 22 publications

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“…12 Briefly, following anesthetic induction, mice were intubated, and a surgical plane of anesthesia was maintained by nosecone with a 2% isoflurane/oxygen mixture. The descending thoracic aorta was exposed through a left thoracotomy, and the baseline aortic outer diameter was measured by video micrometry and recorded as described previously.…”

Section: Operative Proceduresmentioning

confidence: 99%

Alterations in Aortic Cellular Constituents during Thoracic Aortic Aneurysm Development

Jones

Beck

Barbour

et al. 2009

The American Journal of Pathology

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The present study tested the hypothesis that changes in the resident endogenous cellular population accompany alterations in aortic collagen and elastin content during thoracic aortic aneurysm (TAA) development in a murine model. Descending thoracic aortas were analyzed at various time points (2, 4, 8, and 16 weeks) post-TAA induction (0.5 M CaCl 2 , 15 minutes). Aortic tissue sections were subjected to histological staining and morphometric analysis for collagen and elastin, as well as immunostaining for cell-type-specific markers to quantify fibroblasts, myofibroblasts, and smoothmuscle cells. Results were compared with reference control mice processed in the same fashion. Aortic dilatation was accompanied by changes in the elastic architecture that included: a decreased number of elastic lamellae (from 6 to 4); altered area fraction of elastin (elevated at 4 weeks and decreased at 16 weeks); and a decreased area between elastic lamellae (minimum reached at 4 weeks). Total collagen content did not change over time. Increased immunoreactivity for fibroblast and myofibroblast markers was observed at 8-and 16-week post-TAA-induction, whereas immunoreactivity for smooth-muscle cell markers peaked at 4 weeks and returned to baseline by 16 weeks. Therefore, this study demonstrated that changes in aortic elastin content were accompanied by the emergence of a subset of fibroblast-derived myofibroblasts whose altered phenotype may play a significant role in TAA development through the enhancement of extracellular matrix proteolysis.

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Section: Operative Proceduresmentioning

confidence: 99%

Alterations in Aortic Cellular Constituents during Thoracic Aortic Aneurysm Development

Jones

Beck

Barbour

et al. 2009

The American Journal of Pathology

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“…Therefore, the aneurismal model suitable for the research of branched stent graft should be adjacent to or involve the left subclavian artery. Recently, all the animal models of thoracic aortic aneurysm were situated in the medial segment of the descending thoracic aorta, [14][15][16] while the average distance between our aneurismal model and the left subclavian artery was 8.29 ± 0.91 mm. Thus, the aneurismal model is suitable for the research of new branched stent grafts.…”

Section: Discussionmentioning

confidence: 88%

“…14) Ikonomidis et al used calcium chloride to create thoracic aortic aneurysms in mice. 15) Kajimoto et al used jugular vein patches to create thoracic aortic aneurysms in beagles. 16) For Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Mouse models of thoracic aortic aneurysm are too small for the research of endovascular technique. 15) While the canine model of thoracic aortic aneurysm was ideal animal model for the research of endovascular technique for the suitable diameter of the artery, tolerance to surgical trauma. The acquirement of jugular vein patches by Kajimoto et al increased the extent of trauma and risk for infection.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the thoracic aorta was completely clamped in the surgical procedure of other aneurismal models. [14][15][16] That may increase the risk of distal infarction. We clamped the anterolateral wall of the thoracic aorta.…”

Section: Discussionmentioning

confidence: 99%

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A Canine Model of Proximal Descending Thoracic Aortic Aneurysm Created with an Autologous Pericardial Patch

et al. 2013

ATCS

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Objectives: To establish an animal model of proximal descending thoracic aortic aneurysm for the study of branched stent grafts. Materials and Methods: Eleven mongrel dogs underwent the surgical procedure during which an autologous pericardial patch was sewn onto a longitudinal incision in the anterolateral wall of the thoracic aorta near the left subclavian artery to create an artificial thoracic aortic aneurysm. Results: All eleven animals survived the surgical procedure. One animal died from rupture at the surgical site during the first week after surgery. The distance between the artificial aneurysm and the left subclavian artery was 8.29 ± 0.91 mm. The average diameter of the artificial aneurysms did not significantly change over the 4-month follow-up period. Conclusion: A canine model for proximal descending thoracic aortic aneurysm can be achieved using a safe and convenient method. The model can be used in the study of new branched stent graft applied to the aortic arch.

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