A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7

Abstract

B1-type human adenoviruses (HAdVs) HAdV-3, HAdV-7, and HAdV-55 have caused epidemics in North America, Asia, and Europe. However, to date, no adenovirus vaccines or antiviral drugs have been approved for general use. In the present work, a scFv-phage immune library was constructed and mouse monoclonal antibody (MMAb) 10G12 was obtained through selection. 10G12 is specific for HAdV-7 and binds the hexon loop1 and loop2 (LP12), resulting in strong neutralization activity against HAdV-7. Additionally, it is stable in serum and buffer at various pH values. The findings provide insight into adenovirus and antibody responses and may facilitate the design and development of adenovirus vaccines and antiviral drugs.

A humanized anti-human adenovirus 55 monoclonal antibody with good neutralization ability

“…The hexon LP12 fragment and fiber-encoding genes were amplified by PCR and introduced into the pTIG-TRX vector, respectively. Escherichia coli BL21 (DE3) cells were transformed with the pTIG-TRX-LP12/fiber plasmid to produce the His-tagged fusion protein, which was described in our previous study ( 27 ). As coating antigens for ELISA, HAdV55 virions, fragment LP12 (comprising loop1 and loop2 of the hexon), and fiber were employed.…”

“…1) and HAdV7 GZ6965 strain (GenBank accession no. AF532578.1) were maintained in our laboratory ( 27 ). All HAdVs were grown in A549 cells cultured in DMEM with 2% FBS.…”

“…A previously published method ( 27 ) was used to produce and screen scFv colonies directed against HAdV55. Briefly, we immunized female BALB/c mice (6-8 weeks old) intraperitoneally using purified HAdV55 (in phosphate buffered saline (PBS)) mixed with a 1/10 volume of aluminum adjuvant every 14 days for a total of 4 times.…”

Generation and characterization of humanized synergistic neutralizing antibodies against SARS‐CoV‐2

“…The antibody eukaryotic expression vectors, pTSE‐G1n and pTSE‐K, which contain the constant region of the human heavy chain and light chain, respectively, were used to create the complete antibody molecules as previously described. 10 pNL4.3‐luc‐R − E − , a plasmid encoding an Env‐defective, luciferase‐expressing HIV‐1 genome, was kindly provided by Prof. Lihua Hou. pCAGGS‐WSS was constructed to encode the wild‐type SARS‐CoV‐2 S protein (deleting the 19AA of the C‐terminal), which was constructed for preparation of the pseudovirus.…”

“…Phagemid pADSCFV‐S was used to construct a single‐chain antibody fragment (scFv) antibody phage library. The antibody eukaryotic expression vectors, pTSE‐G1n and pTSE‐K, which contain the constant region of the human heavy chain and light chain, respectively, were used to create the complete antibody molecules as previously described 10 . pNL4.3‐luc‐R − E − , a plasmid encoding an Env‐defective, luciferase‐expressing HIV‐1 genome, was kindly provided by Prof. Lihua Hou.…”