A murine preclinical syngeneic transplantation model for breast cancer precision medicine

Abstract

This work presents a new preclinical platform for implementation of selected targeted therapeutics for breast cancer patients.

Multi-omics analysis reveals neoantigen-independent immune cell infiltration in copy-number driven cancers

“…3D ). Finally, we immunostained breast cancer tissue sections from a murine-derived syngeneic transplant model 14 for the CTL marker CD8 and found the immunostaining data was in good agreement with our RNAseq CTL score (Supplementary Fig. 3E ).…”

“…Finally, to verify this phenotype we utilized a preclinical murine syngeneic transplantation breast cancer model (Fig. 7a ) 14 . Within this model we found that phosphorylated ATM as quantified by RPPA showed significant positive correlation with CTL levels as determined by immunostaining for the marker CD8 (Fig.…”

“…Previously described murine-derived syngeneic transplant models (MDSTs) models were transplanted into 4–8 week old FVB/N mice 14 . Tumors were fixed in 10% formaldehyde, embedded in paraffin, and sectioned on a Finesse 325 microtome (Thermo Scientific) into 7 μm sections.…”

“…Pan-cancer gene expression, RPPA data, and mutation data were retrieved from cBioPortal 31 , 32 . We also utilized omics data sets for breast cancer PDXs 19 and MDST models 14 . Transcription factor binding information from ENCODE (ENCyclopedia Of DNA Elements) Project 18 was downloaded from Harmonizome 33 .…”

Cancer research with a human touch

“…To address the lack of heterogeneity and pro duce a more humanlike model, biomedical scientist Lorenzo Federico and his colleagues, working in the laboratory of systems biologist Gordon Mills at the University of Texas MD Anderson Cancer Center in Houston, engi neered a collection of transplantable grafts from primary breast tumors in transgenic mice 3 . The procedure yielded 12 new graft lines of mice -mouse models that can reliably pro duce specific types of cancer with a wide array of genetic changes.…”