2022
DOI: 10.3389/fmicb.2022.896075
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A Mutant Era GTPase Suppresses Phenotypes Caused by Loss of Highly Conserved YbeY Protein in Escherichia coli

Abstract: Ribosome assembly is a complex fundamental cellular process that involves assembling multiple ribosomal proteins and several ribosomal RNA species in a highly coordinated yet flexible and resilient manner. The highly conserved YbeY protein is a single-strand specific endoribonuclease, important for ribosome assembly, 16S rRNA processing, and ribosome quality control. In Escherichia coli, ybeY deletion results in pleiotropic phenotypes including slow growth, temperature sensitivity, accumulation of precursors o… Show more

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(2 citation statements)
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“…Overexpression of WT era suppresses the deletion of rbfA , rsgA and ybeY ( Inoue et al, 2003 ; Campbell and Brown, 2008 ; Ghosal et al, 2018 ). The T99I mutation in the era gene also partially suppresses the ybeY deletion ( Babu et al, 2022 ). By contrast, overproduction of the E200K Era mutant in the KH domain exacerbates the rbfA deletion ( Lerner et al, 1995 ; Inoue et al, 2006 ) , while overexpression of ksgA in its turn suppresses the E200K era mutation ( Lu and Inouye, 1998 ).…”
Section: Structure-function Of Era At the Molecular Levelmentioning
confidence: 99%
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“…Overexpression of WT era suppresses the deletion of rbfA , rsgA and ybeY ( Inoue et al, 2003 ; Campbell and Brown, 2008 ; Ghosal et al, 2018 ). The T99I mutation in the era gene also partially suppresses the ybeY deletion ( Babu et al, 2022 ). By contrast, overproduction of the E200K Era mutant in the KH domain exacerbates the rbfA deletion ( Lerner et al, 1995 ; Inoue et al, 2006 ) , while overexpression of ksgA in its turn suppresses the E200K era mutation ( Lu and Inouye, 1998 ).…”
Section: Structure-function Of Era At the Molecular Levelmentioning
confidence: 99%
“…Stand-alone Era and YbeY proteins physically associate with each other in various bacteria ( Vercruysse et al, 2016 ; Wood et al, 2019 ). They also interact genetically: overexpression of WT era ( Ghosal et al, 2018 ) and a spontaneous point mutation in its GTPase domain ( Babu et al, 2022 ) were shown to significantly ameliorate the growth and the ribosomal phenotypes of Δ ybeY E. coli . Since the molecular function of YbeY is tightly connected with the ribosomal protein uS11, in bacteria as in mitochondria ( Vercruysse et al, 2016 ; Summer et al, 2020 ; D’Souza et al, 2021 ), it might be that Era and YbeY cooperate in installing uS11, thereby enabling the platform closure, like it happens in mitochondria ( Summer et al, 2020 ; Liao et al, 2021 ; Harper et al, 2023 ).…”
Section: Era In the Ribosome Assemblymentioning
confidence: 99%