A mutualistic interaction between the bacterium Pseudomonas asplenii and the harmful algal species Chattonella marina (Raphidophyceae)

Park, Bum Soo; Joo, Jae-Hyoung; Baek, Kyung-Duck; Han, Moonsik

doi:10.1016/j.hal.2016.04.006

Cited by 22 publications

(10 citation statements)

References 44 publications

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“…In the +N treatment on day 8, Alteromonadales and Flavobacteriales exhibited a much higher proportion than in the control and other treatments (Figure 5). Phytoplankton generally provide low molecular weight (LMW) organic compounds, such as amino acids, organic acids, carbohydrates, glycolate and sugar alcohols (Bjørrisen, 1988; Hellebust, 1965; Myklestad, 2000), and high molecular weight (HMW) organic compounds, such as polysaccharides, proteins, nucleic acids and lipids, as result of cell lysis or release from viable cells (Azam & Malfatti, 2007; Biddanda & Benner, 1997; Park, Joo, Baek, & Han, 2016; Passow, 2002; Proctor & Fuhrman, 1991). The SAR92 cluster, which was the most dominant group in Alteromonadales under the +N treatment (day 8), can directly utilize HMW organic compounds originating from phytoplankton cells (Sosa, Gifford, Repeta, & DeLong, 2015).…”

Section: Discussionmentioning

confidence: 99%

Response of the bacterioplankton composition to inorganic nutrient loading and phytoplankton in southern Korean coastal waters: A mesocosm study

et al. 2020

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In Korean coastal waters (KCW), nutrient loading from the four major rivers usually peaks in summer, probably due to the large amount of water discharge in rainy seasons, but little is known about the responses of the bacterial community composition to inorganic nutrient loading. To gain deeper understanding of variations in marine microbial biodiversity and function with inorganic nutrient loading, a large‐scale mesocosm (1,000 L) experiment was conducted in southern KCW for 10 days in summer (July 27 through August 6, 2015) and the bacterioplankton community composition (BCC) was investigated using 16S rRNA amplicon pyrosequencing method. The bioassays established in the mesocosm were designed with the following each nutrient dosing treatments: nitrate (+N), phosphate (+P), and nitrate plus phosphate (+NP). Among the three treatments, the +NP and +N treatments exhibited the largest and second‐largest increases in phytoplankton abundance, respectively, whereas there was relatively less variation under the +P treatment. Enhanced growth of phytoplankton not only induced increases in pH and bacterial abundance (p < .05) but also led to changes in the BCC: the orders Flavobacteria, Micrococcales, Oceanospirillales, and Rhodobacterales exhibited increases in relative abundance, whereas Methylophilales, Puniceicoccales, SAR11, SAR116, and SAR86 showed a decreasing trend. Inorganic nutrients may directly contribute to variation in the BCC, as the relative abundance of Sphingobacteriales, containing members that undergo chemotaxis toward inorganic nutrients, was increased by the +N treatment. Given that these findings, changes in phytoplankton abundance due to nutrient addition may be the most crucial component resulting in variation in the BCC.

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Section: Discussionmentioning

confidence: 99%

Response of the bacterioplankton composition to inorganic nutrient loading and phytoplankton in southern Korean coastal waters: A mesocosm study

et al. 2020

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“…Bacteria with growth effects of ≥1.5 and ≤-0.4 are regarded as growth-promoting and algicidal bacteria, respectively (Park et al, 2016). …”

Section: Methodsmentioning

confidence: 99%

Phycospheric Native Bacteria Pelagibaca bermudensis and Stappia sp. Ameliorate Biomass Productivity of Tetraselmis striata (KCTC1432BP) in Co-cultivation System through Mutualistic Interaction

Park

Wang

et al. 2017

Front. Plant Sci.

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Effective sustainable algal cultivation techniques are essential for mass production of the marine microalga Tetraselmis for biofuel and array of co-products. The phycospheric communities affect the microalgal growth and metabolism through various allelochemical and nutrient interactions; hence, their potential to affect the quantity and quality of both biomass and bioproducts is significant. In the present study, we have screened the phycospheric communities of biofuel producing Tetraselmis striata (KCTC1432BP). A total of 26 bacterial strains were isolated and identified from the phycosphere of T. striata mass culture. Then, each bacterial strain was tested in co-cultivation conditions with T. striata for evaluating its growth promoting and inhibitory effects. Among these all strains, two promising strains (Pelagibaca bermudensis KCTC 13073BP and Stappia sp. KCTC 13072BP) were selected because of their maximum growth promoting effects and mutualistic interactions. The growth rate, biomass productivity, lipid contents, and fatty acids were analyzed during their combined growth in O3 media and compared with axenic growth of T. striata. Later, growth promoting mechanisms in the co-cultivation environment were investigated for these promising bacterial strains under replete and limited conditions of nutrients (nitrate, phosphate, and vitamin B12). The growth promoting potential of P. bermudensis was illustrated by the two fold enhancement in biomass productivity. These bacteria are promising for microalgal cultivation without any negative effects on the native seawater bacterial communities, as revealed by next generation sequencing analysis. This study represents, to date, the first report highlighting the role of phycospheric growth promoting bacteria of promising biofuel feedstock T. striata.

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“…In turn, bacteria provide hormones, vitamins, minerals, and carbon dioxide to algae, thus playing important roles in algal morphogenesis, growth, immune defense, and even spore release and germination (Matsuo et al, 2003; Croft et al, 2005; Singh and Reddy, 2014; Cho et al, 2015; Kouzuma and Watanabe, 2015). In addition, the associated bacteria also greatly contribute to algal bloom initiation and maintenance by secreting growth-promoting substances (Park et al, 2016), participating in nitrogen metabolism through nitrogen fixation (Falcon et al, 2002), or dissimilatory nitrate reduction to ammonium (DNRA), which would benefit the blooming of algal host from nutrient competition (An and Gardner, 2002). Therefore, it was suggested that the analyzing on associated bacteria may provide new insights into the causes and dynamics of seaweed blooming (Fu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Community Structure of Bacteria Associated With Drifting Sargassum horneri, the Causative Species of Golden Tide in the Yellow Sea

Mei

Zhao

et al. 2019

Front. Microbiol.

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Golden tides dominated by Sargassum spp. are occurring at an accelerated rate worldwide. In China, Sargassum has started to bloom in the Yellow Sea and led to tremendous economic losses, but the underlying biological causes and mechanisms are still unclear. Although algae-associated bacteria were suggested to play crucial roles in algal blooms, the profiles of bacteria associated with drifting Sargassum remain unexplored. In this study, the community structures and functions of Sargassum -associated bacteria were analyzed using the high-throughput sequencing data of the V5–V7 hypervariable region of the 16S rRNA gene. Molecular identification revealed that the golden tide analyzed in the Yellow Sea was dominated by a single species, Sargassum horneri . They were a healthy brown color nearshore but were yellow offshore with significantly decreased chlorophyll contents ( P < 0.01), which indicates that yellow S. horneri was under physiological stress. The structural and functional analyses of bacterial communities indicated that the drifting S. horneri had an obvious selectivity on their associated bacteria against surrounding seawater. Although the bacterial communities phylogenetically differed between brown and yellow S. horneri ( P < 0.01), their dominant functions were all nitrogen and iron transporters, which strongly indicates microbial contribution to blooming of the algal host. For the first time, potential epiphytic and endophytic bacteria associated with Sargassum were independently analyzed by a modified co-vortex method with silica sand. We showed that the composition of dominant endophytes, mainly Bacillus and Propionibacterium , was relatively consistent regardless of host status, whereas the epiphytic operational taxonomic units (OTUs) greatly varied in response to weakness of host status; however, dominant functions were consistent at elevated intensities, which might protect the host from stress related to nitrogen or iron deficiency. Thus, we propose that host physiological status at different intensities of functional demands, which were related to variable environmental conditions, may be a critical factor that influences the assembly of epiphytic bacterial communities. This study provided new insight into the structure and potential functions of associated bacteria with golden tide blooms.

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A mutualistic interaction between the bacterium Pseudomonas asplenii and the harmful algal species Chattonella marina (Raphidophyceae)

Cited by 22 publications

References 44 publications

Response of the bacterioplankton composition to inorganic nutrient loading and phytoplankton in southern Korean coastal waters: A mesocosm study

Response of the bacterioplankton composition to inorganic nutrient loading and phytoplankton in southern Korean coastal waters: A mesocosm study

Phycospheric Native Bacteria Pelagibaca bermudensis and Stappia sp. Ameliorate Biomass Productivity of Tetraselmis striata (KCTC1432BP) in Co-cultivation System through Mutualistic Interaction

Community Structure of Bacteria Associated With Drifting Sargassum horneri, the Causative Species of Golden Tide in the Yellow Sea

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