A Mycorrhizal Bacteria Strain Isolated From Polyporus umbellatus Exhibits Broad-Spectrum Antifungal Activity

Abstract

The microbes in the rhizosphere (or mycorrhizosphere) could promote plant growth, however, it is unclear whether mycorrhizosphere microbes could fight multiple fungal pathogens. In this study, twenty-one bacterial strains distributed in 6 genera, including 5 Pseudomonas strains, were isolated from mycorrhizal samples of Polyporus umbellatus that rely on other fungi during their life cycles. Further screening and pot experiments showed that the Pseudomonas strain ZL8 not only inhibited the growth of phytopathogenic fungi, but also promoted the growth of Salvia miltiorrhiza through inhibiting its wilting. In addition, strain ZL8 was found to have the ability to dissolve phosphate, produce IAA and siderophore. Nineteen compounds were identified from the fermentation broth of strain ZL8, of which 2,4-diacetylphloroglucinol (DAPG) had a significant inhibitory effect on phytopathogenic fungi with a minimum inhibitory concentration of 3.12–25 μg/mL. Molecular docking predicted that DAPG could bind to myosin I at two unique sites, which may be responsible to the inhibition of fungal growth. The evaluation results showed that strain ZL8 can be used to develop a dual-purpose biocontrol agents and biofertilizer. These results also provide new insights into the discovery and utilization of new resources for biocontrol agents and biolfertilizers.

Plant growth–promoting rhizobacteria: Peribacillus frigoritolerans 2RO30 and Pseudomonas sivasensis 2RO45 for their effect on canola growth under controlled as well as natural conditions

“…Fengycin is a lipopeptide producing by Bacillus amyloliquefaciens PPL, which contributes to antifungal activity against Fusarium oxysporum ( Kang et al., 2020 ). Arylpolyene from Vibrio fischeri ES114 (APE Vf) was found in many Pseudomonas species ( Gaete et al., 2022 ; Han et al., 2022 ) and according to Dutta et al. (2020) this lipid influences the antifungal effect of P. fluorescens NBC275.…”

“…Fengycin is a lipopeptide producing by Bacillus amyloliquefaciens PPL, which contributes to antifungal activity against Fusarium oxysporum (Kang et al, 2020). Arylpolyene from Vibrio fischeri ES114 (APE Vf) was found in many Pseudomonas species (Gaete et al, 2022;Han et al, 2022) and according to Dutta et al (2020) this lipid influences the antifungal effect of P. fluorescens NBC275. Strain Streptomyces rochei FS18 producing polyketide antibiotic-lankacidin and its derivatives, showed significant antifungal effect against Aspergillus niger (Cao et al, 2015).…”

Isolation, Antimicrobial Effect and Metabolite Analysis of Bacillus amyloliquefaciens ZJLMBA1908 against Citrus Canker Caused by Xanthomonas citri subsp. citri

“…Efficient broad-spectrum antagonist bacteria were screened using the plate confrontation method [16,17]. The strain X. citri subsp.…”

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

“…Soil is composed of mineral particles, organic matter, and microorganisms; the coevolution of microorganisms and their effects on fungal plant pathogens remain unclear (Joo and Hussein, 2022). However, the antimicrobial activity of antagonistic microbes (i.e., Bacillus, Pseudomonas, Agrobacterium, and Actinomycetes) and their natural products show great potential for use in sustainable strategies of plant protection and green pesticides development (Chakraborty et al, 2021;Han et al, 2022). The genus Burkholderia, which is widespread in natural environments with host diversity (Compant et al, 2022) produces bongkrek acid and toxoflavin, which can cause food poisoning (Elshafie and Camele, 2021).…”