A Myrtus communis extract enriched in myrtucummulones and ursolic acid reduces resistance of Propionibacterium acnes biofilms to antibiotics used in acne vulgaris

Feuillolay, Catherine; Pécastaings, Sophie; Gac, Céline Le; Fiorini-Puybaret, Christel; Luc, Joëlle; Joulia, P; Roques, Christine

doi:10.1016/j.phymed.2015.11.016

Cited by 47 publications

(44 citation statements)

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“…In addition to acquired resistance, bacterial biofilms might also play a role in C. acnes reduced susceptibility to antibiotherapy and increased resistance to phagocytosis. An intrinsic property of bacteria in biofilms is indeed their increased tolerance to antibiotics, even if the strains forming the biofilm are normally sensitive to antibiotics (see the following article and). Altogether, resistance, virulence factors, restricted access to immune defense cells of the host, poor penetration of antibacterial agents and selection of ‘persister’ cells are among the mechanisms proposed to explain increased tolerance to antibiotics in C. acnes biofilms …”

Section: Acnes Response To Antibioticsmentioning

confidence: 99%

Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates

Dréno

Pécastaings

Corvec

et al. 2018

Acad Dermatol Venereol

428

317

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While the commensal bacterium Propionibacterium acnes (P. acnes) is involved in the maintenance of a healthy skin, it can also act as an opportunistic pathogen in acne vulgaris. The latest findings on P. acnes shed light on the critical role of a tight equilibrium between members of its phylotypes and within the skin microbiota in the development of this skin disease. Indeed, contrary to what was previously thought, proliferation of P. acnes is not the trigger of acne as patients with acne do not harbour more P. acnes in follicles than normal individuals. Instead, the loss of the skin microbial diversity together with the activation of the innate immunity might lead to this chronic inflammatory condition. This review provides results of the most recent biochemical and genomic investigations that led to the new taxonomic classification of P. acnes renamed Cutibacterium acnes (C. acnes), and to the better characterisation of its phylogenetic cluster groups. Moreover, the latest data on the role of C. acnes and its different phylotypes in acne are presented, providing an overview of the factors that could participate in the virulence and in the antimicrobial resistance of acne-associated strains. Overall, this emerging key information offers new perspectives in the treatment of acne, with future innovative strategies focusing on C. acnes biofilms and/or on its acne-associated phylotypes.

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Section: Acnes Response To Antibioticsmentioning

confidence: 99%

Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates

Dréno

Pécastaings

Corvec

et al. 2018

Acad Dermatol Venereol

428

317

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“…7 Myrtacine â (Myrtus communis extract) has been demonstrated to be efficient on C. acnes biofilm alone or combined with antibiotics using in vitro models. 9 Furthermore, by targeting the biofilm, Myrtacine â is able to potentiate the bactericidal activity of antibiotics and reduce C. acnes counts even with strains resistant to erythromycin, in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…These data corroborate the results obtained in vitro in a previous study using a dynamic model of biofilm formation, demonstrating the destructuring effect of Myrtacine â on a mature C. acnes biofilm formed of both sensitive and erythromycine and clindamycine-resistant strains. 9 The formation of C. acnes biofilm is now considered as responsible for the in vivo resistance of C. acnes to the main antimicrobials prescribed in acne vulgaris 8 (B. Dr eno, this issue). We can therefore hypothesise that in vivo, as it was described in vitro, Myrtacine â may impair the formation and/or the persistence of C. acnes biofilm in the pilosebaceous follicle, leading to an increased sensitivity of bacterial cells to the immune systemeven though the total load of C. acnes is not modified in the patient skin.…”

mentioning

confidence: 99%

Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine^®

Pécastaings

Roques

Nocera

et al. 2018

Acad Dermatol Venereol

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Objective Our main objective was to compare Cutibacterium acnes (C. acnes) skin colonisation in patients with mild to moderate acne versus healthy controls and secondly, to evaluate a Myrtacine â -based cream on C. acnes total population and antibioresistant Cutibacteria in patients with acne.Methods In 60 acne patients (Global Acne Severity Scale, GEA grades 2-3), of mean age 20 [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] Results We first showed (i) high and similar levels of C. acnes colonisation in superficial pilosebaceous follicles and detection of EryR and ClnR strains in both acne and control groups; (ii) different repartition of phylotypes in acne patients versus healthy control, with a predominance of phylotype IA in acne patients and a link between phylotype IA and erythromycin resistance. Besides, after treatment with the Myrtacine â -based cream in acne patients, there was no change in C. acnes total load, but a significant decrease of EryR Cutibacteria, reduced porphyrin production by C. acnes, a decrease in acne severity (GEA), associated with reduced retentional and inflammatory lesions.Conclusion Cutibacterium acnes colonisation was not significantly different in acne versus control groups. Phylotype IA was predominant in acne patient and in EryR C. acnes. A Myrtacine â -based cream significantly reduced the level of EryR Cutibacteria in vivo and improved acne lesions.

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“…and Prunell avulgaris L. (Cheung et al, 2006;Cheung and Zhang, 2008). It exhibits a broad range of pharmacological effects such as anti-inflammatory, antiviral, antioxidant, and hepatoprotective activities (Feuillolay et al, 2016;Kashyap et al, 2016;Siegel et al, 2016), and has proven to be effective in treating various types of cancers including CRC (Pathak et al, 2007;Prasad et al, 2012;Lin et al, 2013;Kadioglu and Efferth, 2015). Several mechanisms by which UA acts as a chemosensitizer have been proposed, such as pro-apoptosis (Meng et al, 2015) and anti-angiogenesis (Lin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

Shan

Xuan

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To explore the efficacy of ursolic acid in sensitizing colon cancer cells to chemotherapy under hypoxia and its underlying mechanisms. Methods: Three colon cancer cell lines (RKO, LoVo, and SW480) were used as in vitro models. 5-Fluorouracil (5-FU) and oxaliplatin were used as chemotherapeutic drugs. Cell viability and apoptosis were tested to evaluate the sensitivity of colon cancer cells to chemotherapy. The transcription and expression levels of hypoxia-inducible factor-1α (HIF-1α), multidrug resistance gene 1 (MDR1), and vascular endothelial growth factors (VEGF) were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and immunoblotting. Cycloheximide and MG132 were used to inhibit protein synthesis and degradation, respectively. In vitro tube formation assay was used to evaluate angiogenesis. Results: We demonstrated the chemosensitizing effects of ursolic acid with 5-FU and oxaliplatin in three colon cancer cell lines under hypoxia. This effect was correlated to its inhibition of MDR1 through HIF-1α. Moreover, ursolic acid was capable of inhibiting HIF-1α accumulation with little effects on its constitutional expression in normoxia. In addition, ursolic acid also down-regulated VEGF and inhibited tumor angiogenesis. Conclusions: Ursolic acid exerted chemosensitizing effects in colon cancer cells under hypoxia by inhibiting HIF-1α accumulation and the subsequent expression of the MDR1 and VEGF.

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A Myrtus communis extract enriched in myrtucummulones and ursolic acid reduces resistance of Propionibacterium acnes biofilms to antibiotics used in acne vulgaris

Abstract: The efficiency of Myrtacine(®) New Generation on P. acnes biofilm alone or combined with antibiotics was demonstrated and can lead to consider it as a potent adjunctive product efficient during the antibiotic course for acne vulgaris treatment.

Cited by 47 publications

References 30 publications

Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates

Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates

Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine^®

Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

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A Myrtus communis extract enriched in myrtucummulones and ursolic acid reduces resistance of Propionibacterium acnes biofilms to antibiotics used in acne vulgaris

Abstract: The efficiency of Myrtacine(®) New Generation on P. acnes biofilm alone or combined with antibiotics was demonstrated and can lead to consider it as a potent adjunctive product efficient during the antibiotic course for acne vulgaris treatment.

Cited by 47 publications

References 30 publications

Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates

Cutibacterium acnes (Propionibacterium acnes) and acne vulgaris: a brief look at the latest updates

Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine®

Ursolic acid sensitized colon cancer cells to chemotherapy under hypoxia by inhibiting MDR1 through HIF-1α

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Characterisation of Cutibacterium acnes phylotypes in acne and in vivo exploratory evaluation of Myrtacine^®