A Na+/H+ antiporter, K2-NhaD, improves salt and drought tolerance in cotton (Gossypium hirsutum L.)

Guo, Wenfang; Li, Gangqiang; Wang, Nan; Yang, Caifeng; Zhao, Yanan; Peng, Huakang; Liu, Dehu; Chen, Sanfeng

doi:10.1007/s11103-020-00969-1

Cited by 19 publications

(8 citation statements)

References 52 publications

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“…tumefaciens LBA4404. Hypocotyl explants from cotton seedlings were transformed using previously described methods [ 24 , 25 ]. Cotton hypocotyls and A .…”

Section: Methodsmentioning

confidence: 99%

Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.)

Shang,

Guo,

Liu

et al. 2023

PLoS ONE

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Chemical nitrogen fertilizer can maintain crop productivity, but overuse of chemical nitrogen fertilizers leads to economic costs and environmental pollution. One approach to reduce use of nitrogen fertilizers is to transfer nitrogenase biosynthetic pathway to non-legume plants. Fe protein encoded by nifH and MoFe protein encoded by nifD and nifK are two structural components of nitrogenase. NifB encoded by nifB is a critical maturase that catalyzes the first committed step in the biosynthesis of nitrogenase FeMo-cofactor that binds and reduces N2. Expression of the nifB, nifH, nifD and nifK is essential to generate plants that are able to fix atmospheric N2. In this study, the four genes (nifB, nifH, nifD and nifK) from Paenibacillu polymyxaWLY78 were assembled in plant expression vector pCAMBIA1301 via Cre/LoxP recombination system, yielding the recombinant expression vector pCAMBIA1301-nifBHDK. Then, the four nif genes carried in the expression vector were co-introduced into upland cotton R15 using Agrobacterium tumefaciens-mediated transformation. Homozygous transgenic cotton lines B2, B5 and B17 of T3 generation were selected by PCR and RT-PCR. qRT-PCR showed that nifB, nifH, nifD and nifK were co-expressed in the transgenic cottons at similar levels. Western blotting analysis demonstrated that NifB, NifH, NifD and NifK were co-produced in the transgenic cottons. Co-expression of the four critical Nif proteins (NifB, NifH, NifD and NifK) in cottons represents an important step in engineering nitrogenase biosynthetic pathway to non-legume plants.

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“…tumefaciens LBA4404. Hypocotyl explants from cotton seedlings were transformed using previously described methods [ 24 , 25 ]. Cotton hypocotyls and A .…”

Section: Methodsmentioning

confidence: 99%

Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.)

Shang,

Guo,

Liu

et al. 2023

PLoS ONE

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“…Like the A. thaliana homologue AtSOS1 (Shi et al, 2002), CcSOS1 is regulated by salinity, especially in the roots after stress, and could play an important role in salt tolerance in C. crassum. In rice (Gupta et al, 2021) and cotton (Guo et al, 2020), SOS1 genes were also predicted to express in plasma membrane. To investigate the subcellular localization of StSOS1-13, the cassette encoding StSOS1-13-Green Fluorescent protein (GFP) fusion protein driven by the CaMV 35S promoter (35S::StSOS1-13-GFP) was transformed into Nicotiana benthamiana leaves, and the fluorescence was observed using the confocal microscope.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide characterization of SOS1 gene family in potato (Solanum tuberosum) and expression analyses under salt and hormone stress

et al. 2023

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Salt Overly Sensitive 1 (SOS1) is one of the members of the Salt Overly Sensitive (SOS) signaling pathway and plays critical salt tolerance determinant in plants, while the characterization of the SOS1 family in potato (Solanum tuberosum) is lacking. In this study, 37 StSOS1s were identified and found to be unevenly distributed across 10 chromosomes, with most of them located on the plasma membrane. Promoter analysis revealed that the majority of these StSOS1 genes contain abundant cis-elements involved in various abiotic stress responses. Tissue specific expression showed that 21 of the 37 StSOS1s were widely expressed in various tissues or organs of the potato. Molecular interaction network analysis suggests that 25 StSOS1s may interact with other proteins involved in potassium ion transmembrane transport, response to salt stress, and cellular processes. In addition, collinearity analysis showed that 17, 8, 1 and 5 of orthologous StSOS1 genes were paired with those in tomato, pepper, tobacco, and Arabidopsis, respectively. Furthermore, RT-qPCR results revealed that the expression of StSOS1s were significant modulated by various abiotic stresses, in particular salt and abscisic acid stress. Furthermore, subcellular localization in Nicotiana benthamiana suggested that StSOS1-13 was located on the plasma membrane. These results extend the comprehensive overview of the StSOS1 gene family and set the stage for further analysis of the function of genes in SOS and hormone signaling pathways.

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“…Overexpression of ARGOS genes in Zea mays leads to a reduction in sensitivity to ethylene, and transgenic plants show enhanced drought resistance as well as higher grain yield in well-watered as well as drought conditions (Shi et al 2015). Similarly, transgenic Glycine max plants overexpressing GmWRI1b show higher oil content and improved plant architecture under field conditions (Guo et al 2020). The list for crop improvement for biotic and abiotic stresses, nutritional enhancement, increase in yield, biofuel production, herbicide resistance, etc., through overexpression approach is quite long with new additions at an ever-increasing rate.…”

Section: Expression and Overexpression Of Candidate Genes For Desiredmentioning

confidence: 99%

Breeding and biotechnological interventions for trait improvement: status and prospects

Singh

Prasad

Muthamilarasan

et al. 2020

Planta

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A Na+/H+ antiporter, K2-NhaD, improves salt and drought tolerance in cotton (Gossypium hirsutum L.)

Cited by 19 publications

References 52 publications

Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.)

Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.)

Genome-wide characterization of SOS1 gene family in potato (Solanum tuberosum) and expression analyses under salt and hormone stress

Breeding and biotechnological interventions for trait improvement: status and prospects

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