A NAC transcription factor, EjNAC1 , affects lignification of loquat fruit by regulating lignin

Xu, Qian; Wang, Wenqiu; Zeng, Jiaoke; Zhang, Jing; Grierson, Donald; Li, Xian; X, Yin; Chen, Kunsong

doi:10.1016/j.postharvbio.2015.02.002

Cited by 71 publications

(69 citation statements)

References 54 publications

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“…VND6 and SND1 are distinct gene families based on their protein sequences in many plant species, including P. trichocarpa (6), Arabidopsis (10,11), rice (28), maize (29), banana (30), and loquat (31). These two families act as transactivators with distinct functions in the regulation of SCW differentiation (3,(10)(11)(12).…”

Section: Discussionmentioning

confidence: 99%

Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa

Lin

Chen

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Secondary cell wall (SCW) biosynthesis is the biological process that generates wood, an important renewable feedstock for materials and energy. NAC domain transcription factors, particularly Vascular-Related NAC-Domain (VND) and Secondary Wall-Associated NAC Domain (SND) proteins, are known to regulate SCW differentiation. The regulation of VND and SND is important to maintain homeostasis for plants to avoid abnormal growth and development. We previously identified a splice variant, , derived from as a dominant-negative regulator, which suppresses the transactivation of all family members. PtrSND1-A2 also suppresses the self-activation of the PtrSND1 family members except for its cognate transcription factor, PtrSND1-A2, suggesting the existence of an unknown factor needed to regulate Here, a splice variant, , derived from was discovered that suppresses the protein functions of all family members. PtrVND6-C1 also suppresses the expression of all members, including, demonstrating that PtrVND6-C1 is the previously unidentified regulator of We also found that PtrVND6-C1 cannot suppress the expression of its cognate transcription factor, is suppressed by PtrSND1-A2 Both PtrVND6-C1 and PtrSND1-A2 cannot suppress their cognate transcription factors but can suppress all members of the other family. The results indicate that the splice variants from the and family may exert reciprocal cross-regulation for complete transcriptional regulation of these two families in wood formation. This reciprocal cross-regulation between families suggests a general mechanism among NAC domain proteins and likely other transcription factors, where intron-retained splice variants provide an additional level of regulation.

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Section: Discussionmentioning

confidence: 99%

Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa

Lin

Chen

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…However, until recently, little progress has been made in understanding these specific mechanisms. Fruit firmness and the lignin content of ‘Luoyangqing’ loquat ( Eriobotrya japonica ) increase during cold storage when a chilling injury occurs, and EjMYB1 , EjNAC1, EjAP2‐1 and EjHSF3 were found to involve in lignification in the cold‐injured fruit by activating lignin biosynthesis genes (Xu et al ., , ; Zeng et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

PbrmiR397a regulates lignification during stone cell development in pear fruit

Xue

Yao

Qin

et al. 2018

Plant Biotechnology Journal

132

104

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Lignified stone cells substantially reduce fruit quality. Therefore, it is desirable to inhibit stone cell development using genetic technologies. However, the molecular mechanisms regulating lignification are poorly understood in fruit stone cells. In this study, we have shown that microRNA (miR) miR397a regulates fruit cell lignification by inhibiting laccase (LAC) genes that encode key lignin biosynthesis enzymes. Transient overexpression of PbrmiR397a, which is the miR397a of Chinese pear (Pyrus bretschneideri), and simultaneous silencing of three LAC genes reduced the lignin content and stone cell number in pear fruit. A single nucleotide polymorphism (SNP) identified in the promoter of the PbrmiR397a gene was found to associate with low levels of fruit lignin, after analysis of the genome sequences of sixty pear varieties. This SNP created a TCA element that responded to salicylic acid to induce gene expression as confirmed using a cell-based assay system. Furthermore, stable overexpression of PbrmiR397a in transgenic tobacco plants reduced the expression of target LAC genes and decreased the content of lignin but did not change the ratio of syringyl- and guaiacyl-lignin monomers. Consistent with reduction in lignin content, the transgenic plants showed fewer numbers of vessel elements and thinner secondary walls in the remaining elements compared to wild-type control plants. This study has advanced our understanding of the regulation of lignin biosynthesis and provided useful molecular genetic information for improving pear fruit quality.

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“…For example, the AP2/ERF factors RAP2.1 and RAP2.6 and the C 2 H 2 -type zinc finger STZ/ZAT10 belong to the CBF regulon [61][62]. Furthermore, NAC was also shown to enhance expression during loquat fruit chilling; however, it could not bind directly to promoters of lignin biosynthesis genes [63]. Many MYB-type transcription factors, such as AtMYB85 [64] and AtMYB88 [65,66], increase cold hardiness by CBF-dependent and CBF-independent pathways in apple.…”

Section: Transcriptional Regulation-related Genes Under Freezing Stressmentioning

confidence: 99%

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

Zhou

Zhu

et al. 2020

Preprint

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Background: Low temperature is a major factor influencing the growth and development of Chinese jujube (Ziziphus jujuba Mill.) in cold winter and spring. Little is known about the molecular mechanisms enabling jujube to cope with different freezing stress conditions. To elucidate the freezing-related molecular changes, we conducted comparative transcriptome analyses from the coldsensitive cultivar 'Dongzao' and cold-tolerant cultivar 'Jinsixiaozao' using RNA-Seq.Results: In our study, more than 20,000 genes were detected at chilling (4°C) and freezing (-10°C, -20°C, -30°C and -40°C) stress between the two cultivars. The numbers of differentially expressed genes (DEGs) between the two cultivars were 1831, 2030, 1993, 1845 and 2137 under the five treatments. Functional enrichment analysis suggested that the metabolic pathway, response to stimulus and catalytic activity were significantly enriched under stronger freezing stress. Among the DEGs, nine participated in the Ca 2+ signal pathway, thirty-two were identified to participate in sucrose metabolism, and others were identified to participate in the regulation of ROS, plant hormones and antifreeze proteins. In addition, important transcription factors (WRKY, AP2/ERF, NAC and bZIP) participating in freezing stress were activated under different degrees of freezing stress. Conclusions:Our research first provides a more comprehensive understanding of DEGs involved in freezing stress at the transcriptome level in two Z. jujuba cultivars with different freezing tolerances.These results may help to elucidate the molecular mechanism of freezing tolerance in jujube and also provides new insights and candidate genes for genetically enhancing freezing stress tolerance. BackgroundLow temperature, as an abiotic stress, not only influences the geographical distribution of many important crops but also negatively impacts the productivity and quality of crops every year [1,2].Cold stresses are divided into chilling (0-15℃) and freezing (<0℃) damage. Freezing stress also has a large impact on the survival and geographical distribution of plants [3]. More than half of the 350,000 plant species on Earth are grown in the tropics and subtropical regions, such as rice, maize, and tomato which are chilling sensitive. However, plants can increase their freezing tolerance by being exposed to chilling, a process known as cold acclimation [4,5].

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A NAC transcription factor, EjNAC1 , affects lignification of loquat fruit by regulating lignin

Cited by 71 publications

References 54 publications

Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa

Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa

PbrmiR397a regulates lignification during stone cell development in pear fruit

Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars

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