A NAC transcription factor, TaNAC5D-2, acts as a positive regulator of drought tolerance through regulating water loss in wheat (Triticum aestivum L.)

Ma, Jianhui; Tang, Xiaoxiao; Sun, Bo; Wei, Jituo; Ma, Liuyin; Yuan, Meng; Zhang, Daijing; Shao, Yun; Li, Chunxi; Chen, Kun-Ming; Jiang, Lina

doi:10.1016/j.envexpbot.2022.104805

Cited by 15 publications

(26 citation statements)

References 67 publications

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“…The wheat cv. Chinese Spring was used for expression analysis, and wheat seedlings were cultured as described by Ma et al (2022) . At the two-leaf stage, the wheat seedlings were transferred into Hogland solution and were then divided into control and Hogland solution containing 15% PEG-6,000 and 100 μM abscisic acid (ABA) treatment groups, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The leaf and root tissues from the control and treated wheat seedlings were collected with three biological replications for each time point. For drought stress in soil, wheat seedlings were cultured as described by Ma et al (2022) . The leaf tissue was sampled at the point of relative water content (RWC) of soil of 50 and 40% with three biological replications, and the well-watered samples were regarded as the control group.…”

Section: Methodsmentioning

confidence: 99%

“…Soil RWC was measured as our previous description (Ma et al, 2022). All data were obtained from three biological replications.…”

Section: Determination Of Freshmentioning

confidence: 99%

“…Therefore, screening of drought-responsive genes is crucial for further molecular breeding. Many TaNACs have been found to regulate drought response ( Ma et al, 2022 ). TaSNAC4-3A expression is induced by drought stress, and its overexpression in Arabidopsis significantly increased drought tolerance by regulating stomatal aperture ( Mei et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…TaNAC071-A was overexpressed in wheat and was found to positively regulate drought tolerance modulated by TaMYBL1 ( Mao et al, 2021 ). In our previous study, we collected a total of 12 drought-responsive TaNACs; interestingly, 10 of them were TaSNACs ( Ma et al, 2022 ). Therefore, analyzing and selecting drought-responsive TaSNACs is of great significance.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of TaSNAC4-3D in Common Wheat (Triticum aestivum L.) Negatively Regulates Drought Tolerance

Zhang²,

Lv³

et al. 2022

Front. Plant Sci.

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The development and production of bread wheat (Triticum aestivum L.) are widely affected by drought stress worldwide. Many NAC transcription factors (TFs) of stress-associated group (SNAC) are functionally proven to regulate drought tolerance. In this study, we identified 41 TaSNACs that were classified into 14 groups, and the expression of TaSNAC4-3D was induced in the leaf tissue via osmotic or abscisic acid (ABA) treatment. TaSNAC4-3D was localized to the nucleus through the transient expression assay, and the C-terminal region exhibited transcriptional activity via transactivation assays. TaSNAC4-3D was overexpressed in common wheat. The wheat plants with TaSNAC4-3D overexpression was more sensitive to drought stress compared with wild-type (WT) plants. The water loss rate showed no difference between transgenic lines and WT plants. However, drought stress increased H2O2 and O2– accumulation and promoted programmed cell death (PCD) in the leaf tissue of TaSNAC4-3D overexpression lines compared with WT plants. RNA-seq analysis was performed under well-watered and drought conditions, and four strong potential target genes, encoding senescence regulators, were identified by analyzing their promoters containing the NAC recognition sequence (NACRS). Based on these results, our findings revealed that TaSNAC4-3D negatively regulates drought tolerance by inducing oxidative damage in bread wheat.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Soil RWC was measured as our previous description (Ma et al, 2022). All data were obtained from three biological replications.…”

Section: Determination Of Freshmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Overexpression of TaSNAC4-3D in Common Wheat (Triticum aestivum L.) Negatively Regulates Drought Tolerance

Zhang²,

Lv³

et al. 2022

Front. Plant Sci.

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The Wheat Transcriptome and Discovery of Functional Gene Networks

Andleeb,

Milson,

Borrill

2023

Compendium of Plant Genomes

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Gene expression patterns have been a widely applied source of information to start understanding gene function in multiple plant species. In wheat, the advent of increasingly accurate and complete gene annotations now enables transcriptomic studies to be carried out on a routine basis and studies by groups around the world have compared gene expression changes under an array of environmental and developmental stages. However, associating data from differentially expressed genes to understanding the biological role of these genes and their applications for breeding is a major challenge. Recently, the first steps to apply network-based approaches to characterise gene expression have been taken in wheat and these networks have enabled the prediction of gene functions in wheat but only for a handful of traits. Combining advanced analysis methods with better sequencing technology will increase our capacity to place gene expression in wheat in the context of functions of genes that influence agronomically important traits.

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