A Naked-Eye and Fluorescent Dual-Channel Probe for Rapid Detection of Hg²⁺ and Its Multiple Applications

Abstract

A fluorescent probe HMI-Hg 2 ＋ was designed and synthesized to recognize Hg 2 ＋ in dimethyl sulfoxide (DMSO)/ phosphate buffer solution (PBS) (V∶V＝5∶5, pH＝10.17). HMI-Hg 2 ＋ recognized Hg 2 ＋ through the fluorescence signal strengthen, and solution color shifted within a few seconds. Spectral experiments confirmed the advantages of the probe with high selectivity and sensitivity to Hg 2 ＋ . The detection limit was calculated to be 0.22 μmol/L. HMI-Hg 2 ＋ has excellent detection consequences in water samples and seafood samples (fish, shrimp and scallop). Moreover, HMI-Hg 2 ＋ can be applied for the cell imaging of Hg 2 ＋ in MCF-7 and has mitochondria-targeted capabilities. Keywords fluorescent probe; Hg 2 ＋ ; seafood sample; cell imaging ARTICLE