A nano silicon adjuvant enhances inactivated transmissible gastroenteritis vaccine through activation the Toll-like receptors and promotes humoral and cellular immune responses

Jin, Xiaohui; Zheng, Lan-Lan; Song, Meirong; Xu, Weisong; Kou, Ya-Nan; Zhou, Yong; Zhang, Liwei; Zhu, Yan-Ning; Wan, Bo; Wei, Zhanyong; Zhang, Gaiping

doi:10.1016/j.nano.2018.02.010

Cited by 21 publications

(16 citation statements)

References 27 publications

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“…DNA vaccines of TGEV were constructed and showed good humoral, mucosal, and cellular immunogenicity in piglets [5,[20][21][22]. Inactivated TGEV vaccines with adjuvants of CpG DNA or nano silicon enhanced the humoral and cellular immune responses [23]. Among these vaccines, commercial vaccines, including inactivated and attenuated vaccines, have been widely used in China, and the inactivated vaccine is the better choice for its excellent safety and has been given higher priority in pig industry.…”

Section: Discussionmentioning

confidence: 99%

Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine

Zhao

Liu

et al. 2020

Virol J

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Background Transmissible gastroenteritis virus (TGEV) causes enteric infection in piglets, characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. Vaccination is an effective measure to control the disease caused by TGEV. Methods In this study, cell-cultured TGEV HN-2012 strain was inactivated by formaldehyde (FA), β-propiolactone (BPL) or binaryethylenimine (BEI), respectively. Then the inactivated TGEV vaccine was prepared with freund's adjuvant, and the immunization effects were evaluated in mice. The TGEV-specific IgG level was detected by ELISA. The positive rates of CD4+, CD8+, CD4+IFN-γ+, CD4+IL-4+ T lymphocytes were detected by flow cytometry assay. Lymphocyte proliferation assay and gross pathology and histopathology examination were also performed to assess the three different inactivating reagents in formulating TGEV vaccine. Results The results showed that the TGEV-specific IgG level in FA group (n = 17) was earlier and stronger, while the BEI group produced much longer-term IgG level. The lymphocyte proliferation test demonstrated that the BEI group had a stronger ability to induce spleen lymphocyte proliferation. The positive rates of CD4+ and CD8+ T lymphocyte subsets of peripheral blood lymphocyte in BEI group was higher than that in FA group and BPL groups by flow cytometry assay. The positive rate of CD4+IFN-γ+ T lymphocyte subset was the highest in the BPL group, and the positive rate of CD4+IL-4+ T lymphocyte subset was the highest in the FA group. There were no obvious pathological changes in the vaccinated mice and the control group after the macroscopic and histopathological examination. Conclusions These results indicated that all the three experimental groups could induce cellular and humoral immunity, and the FA group had the best humoral immunity effect, while the BEI group showed its excellent cellular immunity effect.

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Section: Discussionmentioning

confidence: 99%

Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine

Zhao

Liu

et al. 2020

Virol J

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show abstract

“…DNA vaccines of TGEV were constructed and showed well immunogenicity of humoral, mucosal, and cellular immunities in piglets [15][16][17][18]. Inactivated TGEV vaccines with adjuvants of CpG DNA or nano silicon were enhanced at the humoral and cellular immune responses [3,19]. Among these vaccines, commercial vaccines, including inactivated and attenuated vaccines, have been widely used in China, and the inactivated vaccine is the better choice for its excellent safety and has been given higher priority in the pig industry.…”

Section: Discussionmentioning

confidence: 99%

Assessments of immune response to vaccines of transmissible gastroenteritis virus inactivated by different agents

Zhao¹,

LinTao²,

Xu³

et al. 2020

Preprint

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Background: Transmissible gastroenteritis virus (TGEV) could cause swine enteric infection, which is characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. Vaccination is an effective measure to control the disease caused by TGEV.Methods: In this study, cell-cultured TGEV HN-2012 strain was inactivated by formaldehyde (FA), β-propiolactone (BPL) or binaryethylenimine (BEI), respectively. Then the inactivated TGEV vaccine was prepared with freund's adjuvant, and the immunization effects were evaluated in mice. The TGEV-specific IgG level was detected by ELISA.Results: The results showed that the FA group (n=17) developed earlier and stronger TGEV-specific IgG level, while the BEI group could produce much longer-term IgG level. Lymphocyte proliferation test demonstrated that the BEI group showed a stronger inducibility of spleen lymphocyte proliferation. The BEI group got higher positive rates of CD4+ and CD8+ T lymphocyte subsets of peripheral blood lymphocyte than that of the FA and BPL groups through flow cytometry assay. The BPL group had the highest positive rate of CD4+IFN-γ+ T lymphocyte subset, while the positive rate of CD4+IL-4+ T lymphocyte subset was got the best in FA group. Moreover, there were no obvious pathological changes between the vaccinated mice and control group by the macroscopic and histopathological examination.Conclusions: These results indicated that the three experimental groups both induced cellular and humoral immunities, and the FA group had better effect on humoral immunity, while the BEI group showed its excellent effect on cellular immunity.

show abstract

“…DNA vaccines of TGEV were constructed and showed good humoral, mucosal, and cellular immunogenicity in piglets [20][21][22][23]. Inactivated TGEV vaccines with adjuvants of CpG DNA or nano silicon enhanced the humoral and cellular immune responses [24]. Among these vaccines, commercial vaccines, including inactivated and attenuated vaccines, have been widely used in China, and the inactivated vaccine is the better choice for its excellent safety and has been given higher priority in pig industry.…”

Section: Discussionmentioning

confidence: 99%

Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine

Zhao

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Background:Transmissible gastroenteritis virus (TGEV) causes enteric infection in piglets, characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. Vaccination is an effective measure to control the disease caused by TGEV. Methods: In this study, cell-cultured TGEV HN-2012 strain was inactivated by formaldehyde (FA), β-propiolactone (BPL) or binaryethylenimine (BEI), respectively. Then the inactivated TGEV vaccine was prepared with freund's adjuvant, and the immunization effects were evaluated in mice. The TGEV-specific IgG level was detected by ELISA. The positive rates of CD4+, CD8+, CD4+IFN-γ+, CD4+IL-4+ T lymphocytes were detected by flow cytometry assay. Lymphocyte proliferation assay and gross pathology and histopathology examination were also performed to assess the three different inactivating reagents in formulating TGEV vaccine.Results: The results showed that the TGEV-specific IgG level in FA group (n=17) was earlier and stronger, while the BEI group produced much longer-term IgG level. The lymphocyte proliferation test demonstrated that the BEI group had a stronger ability to induce spleen lymphocyte proliferation. The positive rates of CD4+ and CD8+ T lymphocyte subsets of peripheral blood lymphocyte in BEI group was higher than that in FA group and BPL groups by flow cytometry assay. The positive rate of CD4+IFN-γ+ T lymphocyte subset was the highest in the BPL group, and the positive rate of CD4+IL-4+ T lymphocyte subset was the highest in the FA group. There were no obvious pathological changes in the vaccinated mice and the control group after the macroscopic and histopathological examination. Conclusions: These results indicated that all the three experimental groups could induce cellular and humoral immunity, and the FA group had the best humoral immunity effect, while the BEI group showed its excellent cellular immunity effect.

show abstract

A nano silicon adjuvant enhances inactivated transmissible gastroenteritis vaccine through activation the Toll-like receptors and promotes humoral and cellular immune responses

Cited by 21 publications

References 27 publications

Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine

Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine

Assessments of immune response to vaccines of transmissible gastroenteritis virus inactivated by different agents

Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine

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