A nanocompartment system (Synthosome) designed for biotechnological applications

Nallani, Madhavan; Benito, Samantha Mariela; Onaca, Ozana; Graff, Alexandra; Lindemann, Marcus; Winterhalter, Mathias; Meier, Wolfgang; Schwaneberg, Ulrich

doi:10.1016/j.jbiotec.2005.10.025

Cited by 105 publications

(135 citation statements)

References 29 publications

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“…From a technical point of view, we also showed that by utilizing two distinct extraction and purification procedures, detergent-assisted membrane extraction and refolding from inclusion bodies, we were able to obtain an engineered FhuA⌬C/⌬4L protein with a closely similar large singlechannel conductance but a slightly different electrical signature. Moreover, customized and redesigned FhuA proteins with well defined biophysical, biochemical, and structural features might also be used in gene delivery, drug loading, and encapsulation techniques for medical biotechnology (41,65,66).…”

Section: Discussionmentioning

confidence: 99%

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Redesign of a Plugged β-Barrel Membrane Protein

Mohammad

Howard

Movileanu

2011

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

“…Purification of the Wild-type FhuA Protein-The wild-type FhuA (WT-FhuA) protein was purified as described previously (41) with the following modifications. The outer membranes were pre-extracted in 20 mM Tris, 1 mM EDTA, 0.1% octylpolyoxoethylene (oPOE), pH 8.0.…”

Section: Methodsmentioning

confidence: 99%

Redesign of a Plugged β-Barrel Membrane Protein

Mohammad

Howard

Movileanu

2011

Journal of Biological Chemistry

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“…Meier and coworkers developed various methods to prepare nanovesicles of triblock copolymers poly(2-methyl-2-oxazoline)-blockpoly(dimethyl siloxane)-block-poly(2-methyl-2-oxazoline) (PMOXA-PDMS-PMOXA), and were able to reconstitute membrane proteins [32] such as OmpF (outer membrane protein F) [34] and FhuA [35]. Another approach is to consider enzymes as hydrophilic blocks and, together with a hydrophobic polymer domain, these generate a bilayer [39].…”

Section: Polymeric Membranesmentioning

confidence: 99%

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

et al. 2011

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Today, demand exists for new systems that can meet the challenges of identifying biological entities rapidly and specifically in diagnostics, developing stable and multifunctional membranes, and engineering devices at the nanometer scale. In this respect, bio-decorated membranes combine the specificity and efficacy of biological entities, such as peptides, proteins, and DNA, with stability and the opportunity to chemically tailor the properties of polymeric membranes. A smart strategy that serves to fulfill biological criteria is required, whereby polymer membranes come to mimic biological membranes and do not disturb but rather enhance the functioning and activity of a biological entity. Different approaches have been developed, exemplified by either planar or vesicular membranes, allowing insertion inside the polymer membrane or anchoring via functionalization of the membrane surface. Inspired by nature, but incorporating the strength provided by chemical design, bio-decorated polymer membranes represent a novel concept with great potential in diagnostics and therapeutics.

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“…[17] In addition, we reconstituted FhuA, a channel protein with a large pore diameter of 39-46 Å elliptical cross-section, which ensured a rapid molecular flux into the cavity of PMOXA-PDMS-PMOXA polymersomes. [18] A step further was realized by genetic or chemical modification of OmpF with specific molecules at key locations to influence the pore permeability, substrate selectivity and to induce membrane responsiveness. Such responsive synthetic membranes allow the design of nanoreactors with triggered activity that depends on the external environment, whilst preserving the vesicular architecture, and maintaining the encapsulated enzyme inside.…”

Section: Introductionmentioning

confidence: 99%

Artificial Organelles: Reactions inside Protein–Polymer Supramolecular Assemblies

Garni

Einfalt

Lomora

et al. 2016

Chimia

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Reactions inside confined compartments at the nanoscale represent an essential step in the development of complex multifunctional systems to serve as molecular factories. In this respect, the biomimetic approach of combining biomolecules (proteins, enzymes, mimics) with synthetic membranes is an elegant way to create functional nanoreactors, or even simple artificial organelles, that function inside cells after uptake. Functionality is provided by the specificity of the biomolecule(s), whilst the synthetic compartment provides mechanical stability and robustness. The availability of a large variety of biomolecules and synthetic membranes allows the properties and functionality of these reaction spaces to be tailored and adjusted for building complex self-organized systems as the basis for molecular factories.

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A nanocompartment system (Synthosome) designed for biotechnological applications

Cited by 105 publications

References 29 publications

Redesign of a Plugged β-Barrel Membrane Protein

Redesign of a Plugged β-Barrel Membrane Protein

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

Artificial Organelles: Reactions inside Protein–Polymer Supramolecular Assemblies

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