A nanocomposite approach to develop biodegradable thermogels exhibiting excellent cell-compatibility for injectable cell delivery

Oyama, Naho; Minami, Hiromasa; Kawano, Daichi; Miyazaki, Masaru; Maeda, Takeshi; Toma, Kazunori; Hotta, Atsushi; Nagahama, Koji

doi:10.1039/c4bm00074a

Cited by 29 publications

(32 citation statements)

References 24 publications

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“…PEG- b -PLGA diblock copolymers with different PLGA molecular weight were synthesized from PEG (1000 g/mol) by a ring-opening polymerization as described in a previous paper ( Figure 1 ) [ 1 , 15 ]. In detail, PEG, LA, and GA were put into a Schlenk flask and dried under vacuum overnight.…”

Section: Methodsmentioning

confidence: 99%

Thermo-Responsive Nanocomposite Hydrogels Based on PEG-b-PLGA Diblock Copolymer and Laponite

et al. 2019

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Poly(ethylene glycol)-b-poly(d,l-lactide-co-glycolide) (PEG-b-PLGA) diblock copolymers are widely known as polymeric surfactants for biomedical applications, and exhibit high solubility in water compared to PLGA-b-PEG-b-PLGA triblock copolymers known as gelation agents. In order to overcome the difficulties in the preparation of thermo-responsive hydrogels based on PLGA-b-PEG-b-PLGA due to the low solubility in water, the fabrication of thermo-responsive hydrogels based on PEG-b-PLGA with high solubility in water was attempted by adding laponite to the PEG-b-PLGA solution. In detail, PEG-b-PLGA with high solubility in water (i.e., high PEG/PLGA ratio) were synthesized. Then, the nanocomposite solution based on PEG-b-PLGA and laponite (laponite/PEG-b-PLGA nanocomposite) was fabricated by mixing the PEG-b-PLGA solutions and the laponite suspensions. By using the test tube inversion method and dynamic mechanical analysis (DMA), it was found that thermo-responsive hydrogels could be obtained by using PEG-b-PLGA, generally known as polymeric surfactants, and that the gelation temperature was around the physiological temperature and could be regulated by changing the solution composition. Furthermore, from the structural analysis by small angle neutron scattering (SANS), PEG-b-PLGA was confirmed to be on the surface of the laponite platelets, and the thermosensitive PEG-b-PLGA on the laponite surface could trigger the thermo-responsive connection of the preformed laponite network.

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Section: Methodsmentioning

confidence: 99%

Thermo-Responsive Nanocomposite Hydrogels Based on PEG-b-PLGA Diblock Copolymer and Laponite

et al. 2019

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“…Table 4 shows a summary of the development history of thermoresponsive nanocomposite hydrogels. Oyama, Maeda, and Nagahama et al first reported on a nanocomposite approach to developing biodegradable thermoresponsive hydrogels with excellent cell compatibility (Figure 7a) [64]. We revealed that thermoresponsive nanocomposite hydrogels with low CGC could be obtained by blending PLGA-PEG-PLGA triblock copolymers and laponite, and that the laponite was able to promote the thermoresponsive formation of networks for PLGA-PEG-PLGA copolymers/micelles.…”

Section: Thermoresponsive Nanocomposite Hydrogels Composed Of Laponitmentioning

confidence: 65%

Structures and Applications of Thermoresponsive Hydrogels and Nanocomposite-Hydrogels Based on Copolymers with Poly (Ethylene Glycol) and Poly (Lactide-Co-Glycolide) Blocks

Maeda

2019

Bioengineering

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Thermoresponsive hydrogels showing biocompatibility and degradability have been under intense investigation for biomedical applications, especially hydrogels composed of hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly(lactic acid-co-glycolic acid) (PLGA) as first-line materials. Even though various aspects such as gelation behavior, degradation behavior, drug-release behavior, and composition effect have been studied for 20 years since the first report of these hydrogels, there are still many outputs on parameters affecting their gelation, structure, and application. In this review, the current trends of research on linear block copolymers composed of PEG and PLGA during the last 5 years (2014-2019) are summarized. In detail, this review stresses newly found parameters affecting thermoresponsive gelation, findings from structural analysis by simulation, small-angle neutron scattering (SANS), etc., progress in biomedical applications including drug delivery systems and regeneration medicine, and nanocomposites composed of block copolymers with PEG and PLGA and nanomaterials (laponite).

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“…[ 21 ] As for the block copolymers of PEG and PLGA, our group has reported the thermogelling nanocomposite hydrogels using PLGA‐ b ‐PEG‐ b ‐PLGA and PEG‐ b ‐PLGA. [ 22–26 ] By using block copolymers with a degradable PLGA block, the controlled degradability was found to be introduced to the nanocomposite hydrogels in addition to the thermogelling behavior. [ 27–33 ] In our previous study on the nanocomposites using PLGA‐ b ‐PEG‐ b ‐PLGA triblock copolymers, the PEG/PLGA ratios (i.e., the weight ratio of the PEG block and the PLGA block) of PLGA‐ b ‐PEG‐ b ‐PLGA were fixed around 0.8, and the thermoresponsive gelation behavior was investigated by varying the total molecular weight of PLGA‐ b ‐PEG‐ b ‐PLGA, whereas the degradation behavior was also investigated by adjusting the LA/GA ratio (i.e., the molar ratio of LA and GA) in the PLGA block.…”

Section: Introductionmentioning

confidence: 99%

Thermogelling Nanocomposite Hydrogel: PLGA Molecular Weight in PLGA‐b‐PEG‐b‐PLGA Affecting the Thermogelling Behavior

Maeda

Tanimoto

Hotta

2021

Macro Chemistry & Physics

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Thermoresponsive gelation of the nanocomposites consisting of poly(lactic acid‐co‐glycolic acid)‐b‐poly(ethylene glycol)‐b‐poly(lactic acid‐co‐glycolic acid) (PLGA‐b‐PEG‐b‐PLGA) triblock copolymers and LAPONITE (LAPONITE/PLGA‐b‐PEG‐b‐PLGA nanocomposites) is investigated by decreasing the molecular weights of PLGA as 1130, 900, and 470 g mol−1. As for the pure triblock copolymers, it is confirmed by UV–vis and SANS that a micellar structure with a hydrophobic core is formed even in the case of PLGA‐b‐PEG‐b‐PLGA with the lowest PLGA molecular weight of 470 g mol−1 (Triblock0.5k). As for the nanocomposites with LAPONITE, it is found that the gelation temperature (Tgel) becomes less dependent on the PLGA‐b‐PEG‐b‐PLGA concentration and more stable as the PLGA molecular weight decreases, and that Tgel of the nanocomposites almost linearly decreases as the LAPONITE concentration increases. Eventually, the nanocomposite with Triblock0.5k reaches Tgel between room temperature and physiological temperature. In addition, gradual degradation behavior is observed for the nanocomposite with Triblock0.5k. Considering the monomeric molecular weights of LA and GA at the LA/GA ratio of ≈2.0, it is concluded that the PLGA‐b‐PEG‐b‐PLGA with the lower PLGA molecular weight (i.e., Triblock0.5k) can be effectively used for thermoresponsive and degradable hydrogels targeting biomedical applications.

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A nanocomposite approach to develop biodegradable thermogels exhibiting excellent cell-compatibility for injectable cell delivery

Cited by 29 publications

References 24 publications

Thermo-Responsive Nanocomposite Hydrogels Based on PEG-b-PLGA Diblock Copolymer and Laponite

Thermo-Responsive Nanocomposite Hydrogels Based on PEG-b-PLGA Diblock Copolymer and Laponite

Structures and Applications of Thermoresponsive Hydrogels and Nanocomposite-Hydrogels Based on Copolymers with Poly (Ethylene Glycol) and Poly (Lactide-Co-Glycolide) Blocks

Thermogelling Nanocomposite Hydrogel: PLGA Molecular Weight in PLGA‐b‐PEG‐b‐PLGA Affecting the Thermogelling Behavior

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