A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to Rat sciatic nerve regeneration across a 30-mm defect bridge

Biazar, Esmaeil; Keshel, Saeed Heidari

doi:10.3109/15419061.2013.774378

Cited by 54 publications

(30 citation statements)

References 24 publications

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“…To achieve positive cellbiomaterial interaction, functional groups or biomacromolecules have been immobilized onto PHAs for direct tuning of surface without altering their bulk properties [1][2][3][4][5][6][7][8]. Electrospinning has been rapidly developed into a technique to prepare nanofibers with the diameter ranging from tens of nanometers to several microns [9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Controlling surface properties is very important for the high performance of adhesion.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of PHBV Nanofibrous Mat by Laminin Protein and Its Cellular Study

Sahebalzamani

Biazar

Shahrezaei

et al. 2014

International Journal of Polymeric Materials and Polymeric Biom

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Nanofibers have improved the performance of biomaterials, and can be considered effective. In this study, PHBV nanofibers were designed and then modified and crosslinked by oxygen plasma and laminin. The samples were evaluated by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM), contact angle, mechanical analyses, and cell culture. ATR-FTIR structural analysis showed the presence of functional groups on the nanofibrous surfaces. The SEM images showed the average size of nanofibers to be about 100 nm for the samples. The 70 difference was obtained in the contact angle analysis, obtained for the laminin-crosslinked nanofibrous mat than the unmodified nanofibrous mat. Cellular investigation showed better adhesion and cell growth and proliferation of laminin-crosslinked nanofibrous samples than other samples. The bioavailability of PHBV fibers with covalently attached laminin was found to be identical to that of PHBV fibers with physically adsorbed laminin, indicating that covalent attachment of protein is a suitable method for enhancing the biocompatibility of tissue engineering scaffolds.

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Section: Introductionmentioning

confidence: 99%

Surface Modification of PHBV Nanofibrous Mat by Laminin Protein and Its Cellular Study

Sahebalzamani

Biazar

Shahrezaei

et al. 2014

International Journal of Polymeric Materials and Polymeric Biom

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“…The success of ANA in combination with SCs has revealed a promising strategy for peripheral nerve injury repair (Gravvanis et al, 2007;Biazar and Heidari Keshel, 2013). SCs are a strong candidate for transplantation in ANA in nerve gap repair due to their effectiveness in axonal regeneration in short distance (Moore et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

KLF7-transfected Schwann cell graft transplantation promotes sciatic nerve regeneration

Wang

Hua

et al. 2017

Neuroscience

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Abstract:Our former study demonstrated that Krüppel-like Factor 7 (KLF7) is a transcription factor that stimulates axonal regeneration after peripheral nerve injury. Currently, we used a gene therapy approach to overexpress KLF7 in Schwann Cells (SCs) and assessed whether KLF7-transfected SCs graft could promote sciatic nerve regeneration. SCs were transfected by AAV2-KLF7 in vitro. Mice was allografted by an acellular nerve (ANA) with either an injection of DMEM (ANA group), SCs (ANA+SCs group) or AAV2-KLF7-transfected SCs (ANA+KLF7-SCs group) to assess repair of a sciatic nerve gap. The results indicate that KLF7 overexpression promoted the proliferation of both transfected SCs and native SCs. The neurite length of the DRG explants was enhanced.Several beneficial effects were detected in the ANA+KLF7-SCs group including an increase in the compound action potential amplitude , sciatic function index score, enhanced expression of PKH26-labeling transplant SCs, peripheral myelin protein 0 , neurofilaments , S-100, and myelinated regeneration nerve.Additionally, HRP-labeled motoneurons in the spinal cord, CTB-labeled sensory neurons in the DRG, motor endplate density and the weight ratios of target muscles were increased by the treatment while thermal hyperalgesia was diminished. Finally, expression of KLF7, NGF, GAP43, TrkA and TrkB were enhanced in the grafted SCs, which may indicate that several signal pathways may be involved in conferring the beneficial effects from KLF7 overexpression. We concluded that KLF7-overexpressing SCs promoted axonal regeneration of the peripheral nerve and enhanced myelination, which collectively proved KLF-SCs as a novel therapeutic strategy for injured nerves.

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“…Nanofi bers have improved the performance of biomaterials. Electro spinning is one of the most important methods for the fabrication of nanofi brous scaffolds (Majdi et al, 2011;Biazar et al, 2010b;Rezaei-Tavirani et al, 2011;Ai et al, 2011;Heidari et al, in press;Biazar et al, 2013;Montazeri et al, 2011). Neuroscience researchers used different methods for detection of functional recovery in regenerated nerve.…”

Section: Introductionmentioning

confidence: 99%

Behavioral evaluation of regenerated rat sciatic nerve by a nanofibrous PHBV conduit filled with Schwann cells as artificial nerve graft

Biazar

Keshel

Pouya

2013

Cell Communication & Adhesion

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The aim of this study is to develop a nanofi brous polymeric nerve conduit with Schwann cells (SCs) and to evaluate its effi ciency on the promotion of functional and locomotive activities in rats. The conduits were implanted into a 30-mm gap in the sciatic nerves of the rats. Four months after surgery, the rats were monitored and evaluated by behavioral analyses such as toe out angle, toe spreading analysis, walking track analysis, extensor postural thrust, open-fi eld analysis, swimming test and nociceptive function, four months post surgery. Four months post-operatively, the results from behavioral analyses demonstrated that in the grafted groups especially in the grafted group with SCs, the rat sciatic nerve trunk had been reconstructed with functional recovery such as walking, swimming and recovery of nociceptive function. This study proves the feasibility of artifi cial conduit with SCs for nerve regeneration by bridging a longer defect in the rat model.

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A nanofibrous PHBV tube with Schwann cell as artificial nerve graft contributing to Rat sciatic nerve regeneration across a 30-mm defect bridge

Cited by 54 publications

References 24 publications

Surface Modification of PHBV Nanofibrous Mat by Laminin Protein and Its Cellular Study

Surface Modification of PHBV Nanofibrous Mat by Laminin Protein and Its Cellular Study

KLF7-transfected Schwann cell graft transplantation promotes sciatic nerve regeneration

Behavioral evaluation of regenerated rat sciatic nerve by a nanofibrous PHBV conduit filled with Schwann cells as artificial nerve graft

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