Measurement of masses of microscale objects or weak force with ultrahigh sensitivity (down to nanogram/piconewton level) and compact configuration is highly desired for fundamental research and applications in various disciplines. Here, by using freestanding gold flakes with high reflectivity (≈98% at 980 nm) as the sample tray and silica microfibers with extremely low spring constant (≈0.05 mN m−1) as the cantilever beams, miniature capacitive balances are reported with piconewton‐level detection limit (picobalances) and reliable radiation force‐based calibration. In the design, the gold flake is suspended by two silica microfibers, which also functions as an electrode to form a capacitor with an underneath gold electrode. Benefitting from the high reflectivity of the gold flake, the performance of picobalances can be precisely calibrated by exerting piconewton‐level radiation pressure on the gold flake (working as a mirror) with a laser, showing a detection limit as low as 6.9 pN. Finally, using a fiber taper‐assisted micromanipulation technique, masses of various types of pollens (with weights ranging from 4.6 to 96.3 ng) are readily measured by a picobalance at single‐particle level. The miniature picobalances should find applications in precise measurement of masses of micro or nanoscale objects and various types of weak forces.