A nanostructure platform for live-cell manipulation of membrane curvature

Li, Xiao; Matino, Laura; Zhang, Wei; Klausen, Lasse Hyldgaard; McGuire, Allister F.; Lubrano, Claudia; Zhao, Wenting; Santoro, Francesca; Cui, Bianxiao

doi:10.1038/s41596-019-0161-7

Cited by 95 publications

(96 citation statements)

References 51 publications

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“…The curvature sensing and membrane rolling induced by ANXA4 and ANXA5, but not ANXA2, could together indicate that trimerization is critical for membrane curvature sensing and curvature induction. Beyond these results, future experiments should test whether the two dimensional curvature of annexins can discriminate between spherical and cylindrical curvatures in similar assays as used in Li et al [37].…”

Section: Annexins Are Recruited By Membrane Curvaturementioning

confidence: 93%

Interdisciplinary Synergy to Reveal Mechanisms of Annexin-Mediated Plasma Membrane Shaping and Repair

Bendix

Simonsen

Florentsen

et al. 2020

Cells

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The plasma membrane surrounds every single cell and essentially shapes cell life by separating the interior from the external environment. Thus, maintenance of cell membrane integrity is essential to prevent death caused by disruption of the plasma membrane. To counteract plasma membrane injuries, eukaryotic cells have developed efficient repair tools that depend on Ca 2+ -and phospholipid-binding annexin proteins. Upon membrane damage, annexin family members are activated by a Ca 2+ influx, enabling them to quickly bind at the damaged membrane and facilitate wound healing. Our recent studies, based on interdisciplinary research synergy across molecular cell biology, experimental membrane physics, and computational simulations show that annexins have additional biophysical functions in the repair response besides enabling membrane fusion. Annexins possess different membrane-shaping properties, allowing for a tailored response that involves rapid bending, constriction, and fusion of membrane edges for resealing. Moreover, some annexins have high affinity for highly curved membranes that appear at free edges near rupture sites, a property that might accelerate their recruitment for rapid repair. Here, we discuss the mechanisms of annexin-mediated membrane shaping and curvature sensing in the light of our interdisciplinary approach to study plasma membrane repair.

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Section: Annexins Are Recruited By Membrane Curvaturementioning

confidence: 93%

Interdisciplinary Synergy to Reveal Mechanisms of Annexin-Mediated Plasma Membrane Shaping and Repair

Bendix

Simonsen

Florentsen

et al. 2020

Cells

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“…Zhao et al investigated how low aspect ratio quartz nanopillars and nanobars can stimulate protein recruitment during clathrin‐mediated endocytosis . (See also their recently reported fabrication and interfacing protocol …”

Section: Understanding the Cell–nanostructure Interfacementioning

confidence: 99%

“…Effective protocols facilitate high‐contrast imaging while minimizing fixation and vacuum artefacts that might artificially deform structures or alter cell–nanostructure distances . These protocols use multi‐stage, heavy‐metal and thin plasticization techniques to ensure clear and accurate imaging . When combined with focused‐ion‐beam milling (a technique sometimes referred to as slice and view), the interior ultrastructure of the cell–nanostructure interface can be reconstructed in detail .…”

Section: Characterization Approachesmentioning

confidence: 99%

“…These protocols use multi‐stage, heavy‐metal and thin plasticization techniques to ensure clear and accurate imaging . When combined with focused‐ion‐beam milling (a technique sometimes referred to as slice and view), the interior ultrastructure of the cell–nanostructure interface can be reconstructed in detail . Recently, Gopal et al have shown how this approach can also be combined with immunogold labeling (whereby antigens are labeled with antibodies conjugated to gold nanoparticles).…”

Section: Characterization Approachesmentioning

confidence: 99%

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High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography on the cellular level and have become useful tools for perturbing and sensing the cellular environment. Motivation comes from the ability of high‐aspect‐ratio nanostructures to deliver cargoes into cells and tissues, access the intracellular environment, and control cell behavior. These structures directly perturb cells' ability to sense and respond to external forces, influencing cell fate, and enabling new mechanistic studies. Through careful design of their nanoscale structure, these systems act as biological metamaterials, eliciting unusual biological responses. While predominantly used to interface eukaryotic cells, there is growing interest in nonanimal and prokaryotic cell interfacing. Both experimental and theoretical studies have attempted to develop a mechanistic understanding for the observed behaviors, predominantly focusing on the cell–nanostructure interface. This review considers how high‐aspect‐ratio nanostructured surfaces are used to both stimulate and sense biological systems.

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“…7 Another type of nanostructured cell culture substrates that has recently attracted a lot of attention, are based on flat and cell compatible supports decorated with vertically aligned nanowires or nanopillars. [8][9][10][11][12] Nanowires or nanopillars are typically 10 nm to 100 nm in diameter 1 µm to 5 µm in length and can be made from carbon, 13,14 SiO, silicon, 15,16 indium arsenide 17 or copper oxide. 18 Fabrication is typically performed using a combination of patterning (UV or EBL), growth (for example molecular beam epitaxy, plasma-enhanced chemical vapour deposition and electrodeposition) or etching (by wet-or dry-etching techniques).…”

Section: Introductionmentioning

confidence: 99%

Electron beam lithography fabrication of SU-8 polymer structures for cell studies

Vinje

Beckwith

Sikorski

2019

Preprint

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Flat surfaces decorated with micro-and nanostructures are important tools in biomedical research used to control cellular shape, in studies of mechanotransduction, membrane mechanics, cell migration and cellular interactions with nanostructured surfaces. Existing methods to fabricate surface-bound nanostructures are typically limited either by resolution, aspect ratio or throughput. In this work, we explore electron beam lithography based structuring of the epoxy resist SU-8 on glass substrate.We focus on a systematic investigation of the process parameters and determine limits of the fabrication process, both in terms of spatial resolution, structure aspect ration and fabrication throughput. The described approach is capable of producing high-aspect ratio, surface bound nanostructures with height ranging from 100 nm to 4000 nm and with in-plane resolution below 100 nm directly on a transparent substrate. Fabricated nanostructured surfaces can be integrated with common techniques for biomedical research, such as high numerical aperture optical microscopy. Further more, we show how the described approach can be used to make nanostructures with multiple heights 1 on the same surface, something which is not readily achievable using alternative fabrication approaches. Our research paves an alternative way of manufacturing nanostructured surfaces with applications in life science research.

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A nanostructure platform for live-cell manipulation of membrane curvature

Cited by 95 publications

References 51 publications

Interdisciplinary Synergy to Reveal Mechanisms of Annexin-Mediated Plasma Membrane Shaping and Repair

Interdisciplinary Synergy to Reveal Mechanisms of Annexin-Mediated Plasma Membrane Shaping and Repair

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

Electron beam lithography fabrication of SU-8 polymer structures for cell studies

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