A natural antisense RNA improves chrysanthemum cold tolerance by regulating the transcription factor <i>DgTCP1</i>

Li, Xin; Qing, Yang; Liao, Xiaoqin; Tian, Yuchen; Zhang, Fan; Zhang, Lei; Liu, Qinglin

doi:10.1093/plphys/kiac267

Cited by 22 publications

(13 citation statements)

References 43 publications

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“…2018 ; Li et al . 2022b ), B3 ( Verma and Bhatia 2019 ), TCP ( Li et al . 2022a ), and GATA ( Zhang et al .…”

Section: Resultsmentioning

confidence: 99%

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Molecular dissection of an intronic enhancer governing cold-induced expression of the vacuolar invertase gene in potato

Zhu,

Chen,

Butler

et al. 2024

The Plant Cell

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Potato (Solanum tuberosum) is the third most important food crop in the world. Potato tubers must be stored at cold temperatures to minimize sprouting and losses due to disease. However, cold temperatures strongly induce the expression of the potato vacuolar invertase gene (VInv) and cause reducing sugar accumulation. This process, referred to as “cold-induced sweetening”, is a major postharvest problem for the potato industry. We discovered that the cold-induced expression of VInv is controlled by a 200-bp enhancer, VInvIn2En, located in its second intron. We identified several DNA motifs in VInvIn2En that bind transcription factors involved in the plant cold stress response. Mutation of these DNA motifs abolished VInvIn2En function as a transcriptional enhancer. We developed VInvIn2En deletion lines in both diploid and tetraploid potato using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-mediated gene editing. VInv transcription in cold-stored tubers was significantly reduced in the deletion lines. Interestingly, the VInvIn2En sequence is highly conserved among distantly related Solanum species, including tomato (Solanum lycopersicum) and other non-tuber-bearing species. We conclude that the VInv gene as well as the VInvIn2En enhancer have adopted distinct roles in the cold stress response in tubers of tuber-bearing Solanum species.

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“…2018 ; Li et al . 2022b ), B3 ( Verma and Bhatia 2019 ), TCP ( Li et al . 2022a ), and GATA ( Zhang et al .…”

Section: Resultsmentioning

confidence: 99%

“…2021 ). A TCP1 TF in Chrysanthemum morifolium , DgTCP1, was induced by cold temperature and can regulate peroxidase activity and reduce ROS accumulation ( Li et al . 2022a ).…”

Section: Discussionmentioning

confidence: 99%

Molecular dissection of an intronic enhancer governing cold-induced expression of the vacuolar invertase gene in potato

Zhu,

Chen,

Butler

et al. 2024

The Plant Cell

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“…lncRNAs also have roles in heat, cold, drought, osmotic and salt tolerance in wheat ( TalnRNA27 and TalnRNA5 ) (Xin et al, 2011), Chinese cabbage (TCONS_00048391) (Wang et al, 2019), Populus ( PROMTS ) (Song et al, 2019), chrysanthemum ( NAT of DgTCP1 ) (Li et al, 2022b), and Arabidopsis ( BLIL1 , CIL1 , DRIR , and asDOG1 ) (Qin et al, 2017; Yatusevich et al, 2017; Sun et al, 2020c; Liu et al, 2022a). Taken together, the above examples show that lncRNAs may be used as good targets to improve the disease resistance and stress resistance of crop species.…”

Section: Regulatory Lncrnas In Plantsmentioning

confidence: 99%

From molecular basics to agronomic benefits: Insights into noncoding RNA‐mediated gene regulation in plants

Wang

Deng

Zhu

2022

JIPB

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The development of plants is largely dependent on their growth environment. To better adapt to a particular habitat, plants have evolved various subtle regulatory mechanisms for altering gene expression. Non coding RNAs (ncRNAs) constitute a major portion of the transcriptomes of eukaryotes. Various ncRNAs have been recognized as important regulators of the expression of genes involved in essential biological processes throughout the whole life cycles of plants. In this review, we summarize the current understanding of the biogenesis and contributions of small nucle olar RNA (snoRNA)‐ and regulatory long non coding RNA (lncRNA)‐mediated gene regulation in plant development and environmental responses. Many regulatory ncRNAs appear to be associated with increased yield, quality and disease resistance of various species and cultivars. These ncRNAs may potentially be used as genetic resources for improving agronomic traits and for molecular breeding. The challenges in understanding plant ncRNA biology and the possibilities to make better use of these valuable gene resources in the future are discussed in this review.

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“…In chrysanthemum, the expression levels of most CnTCPs were down‐regulated under cold conditions, and CnTCP4 overexpression plants led to hypersensitivity to cold stress (Tian et al ., 2022 ). The expression levels of DgPOD (peroxidase gene from chrysanthemum) were up‐regulated in DgTCP1 overexpression plants after cold stress, indicating that DgTCP1 positively regulates freezing tolerance by reducing the reactive oxygen species accumulation (Li et al ., 2022 ). Interestingly, a cysteine (Cys‐20) site in the TCP‐conserved domain of the AtTCP15 protein is oxidized under oxidative stress and inhibits its transcription, suggesting that TCP is also involved in ROS‐mediated signal transduction (Viola et al ., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Histone deacetylase MdHDA6 is an antagonist in regulation of transcription factor MdTCP15 to promote cold tolerance in apple

Guo

Wang

et al. 2023

Plant Biotechnology Journal

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SummaryUnderstanding the molecular regulation of plant cold response is the basis for cold resistance germplasm improvement. Here, we revealed that the apple histone deacetylase MdHDA6 can perform histone deacetylation on cold‐negative regulator genes and repress their expression, leading to the positive regulation of cold tolerance in apples. Moreover, MdHDA6 directly interacts with the transcription factor MdTCP15. Phenotypic analysis of MdTCP15 transgenic apple lines and wild types reveals that MdTCP15 negatively regulates cold tolerance in apples. Furthermore, we found that MdHDA6 can facilitate histone deacetylation of MdTCP15 and repress the expression of MdTCP15, which positively contributes to cold tolerance in apples. Additionally, the transcription factor MdTCP15 can directly bind to the promoter of the cold‐negative regulator gene MdABI1 and activate its expression, and it can also directly bind to the promoter of the cold‐positive regulator gene MdCOR47 and repress its expression. However, the co‐expression of MdHDA6 and MdTCP15 can inhibit MdTCP15‐induced activation of MdABI1 and repression of MdCOR47, suggesting that MdHDA6 suppresses the transcriptional regulation of MdTCP15 on its downstream genes. Our results demonstrate that histone deacetylase MdHDA6 plays an antagonistic role in the regulation of MdTCP15‐induced transcriptional activation or repression to positively regulate cold tolerance in apples, revealing a new regulatory mechanism of plant cold response.

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A natural antisense RNA improves chrysanthemum cold tolerance by regulating the transcription factor DgTCP1

Cited by 22 publications

References 43 publications

Molecular dissection of an intronic enhancer governing cold-induced expression of the vacuolar invertase gene in potato

Molecular dissection of an intronic enhancer governing cold-induced expression of the vacuolar invertase gene in potato

From molecular basics to agronomic benefits: Insights into noncoding RNA‐mediated gene regulation in plants

Histone deacetylase MdHDA6 is an antagonist in regulation of transcription factor MdTCP15 to promote cold tolerance in apple

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