A Natural Cattle Immune Response Against Horn Fly (Diptera: Muscidae) Salivary Antigens May Regulate Parasite Blood Intake

Breijo, Martı́n; Pastro, Lucía; Rocha, Sérgio Paulo Dejato da; Ures, X.; Alonzo, P.; Santos, Michelle Cardoso dos; Bolatto, Carmen; Fernández, Cecilia; Meikle, A.

doi:10.1093/jee/tow133

Cited by 10 publications

(11 citation statements)

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“…In Tabanus yao , a member of this family acquired a disintegrins RGD motif that inhibits platelet aggregation by inhibiting the interaction of platelets with fibrinogen [51], and another incorporated a RTS domain and inhibits angiogenesis [52]. The contig named ab-48610_FR5_181-357 (Additional file 3) coded for a truncated member of the antigen 5 family, previously described as a strong H. irritans salivary antigen [53], and had collected the highest number of reads, thus with an E.F. value of 100. The full transcript was recovered when the abyss and the trinity assemblies were further assembled together (Additional file 1: Figure S1), indicating this strategy to be the best to recover full transcripts of highly expressed messages.…”

Section: Resultsmentioning

confidence: 99%

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An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

et al. 2019

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Background The horn fly ( Haematobia irritans ) is an obligate blood feeder that causes considerable economic losses in livestock industries worldwide. The control of this cattle pest is mainly based on insecticides; unfortunately, in many regions, horn flies have developed resistance. Vaccines or biological control have been proposed as alternative control methods, but the available information about the biology or physiology of this parasite is rather scarce. Results We present a comprehensive description of the salivary and midgut transcriptomes of the horn fly ( Haematobia irritans ), using deep sequencing achieved by the Illumina protocol, as well as exploring the virome of this fly. Comparison of the two transcriptomes allow for identification of uniquely salivary or uniquely midgut transcripts, as identified by statistically differential transcript expression at a level of 16 x or more. In addition, we provide genomic highlights and phylogenetic insights of Haematobia irritans Nora virus and present evidence of a novel densovirus, both associated to midgut libraries of H. irritans . Conclusions We provide a catalog of protein sequences associated with the salivary glands and midgut of the horn fly that will be useful for vaccine design. Additionally, we discover two midgut-associated viruses that infect these flies in nature. Future studies should address the prevalence, biological effects and life cycles of these viruses, which could eventually lead to translational work oriented to the control of this economically important cattle pest. Electronic supplementary material The online version of this article (10.1186/s12864-019-5984-7) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…These transcripts are a mining field for anti- Haematobia vaccine development. One of the salivary transcripts have already been used for this purpose [19, 53].…”

Section: Discussionmentioning

confidence: 99%

An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

et al. 2019

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“…HTB (GenBank: AJY26992.1) was identified in a cDNA library prepared from H. irritans salivary glands. The production of recombinant HTB was performed as described [ 18 ]. Briefly, the sequence coding for the predicted mature HTB was amplified by PCR from a recombinant plasmid carrying the full-length cDNA derived from an oligo-capped full-length enriched library prepared from the H. irritans salivary gland mRNA.…”

Section: Methodsmentioning

confidence: 99%

Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages

et al. 2018

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BackgroundThe horn fly Haematobia irritans is a blood-sucking ectoparasite responsible for substantial economic loss of livestock. Like other hematophagous arthropods species, the successful blood-feeding of H. irritans is highly dependent on the modulation of the host’s hemostasis and immune system. Here, we evaluated the biological activity of hematobin (HTB), a protein recently identified in the H. irritans saliva, on macrophage biology. The goal was to understand the putative interactions between the components of H. irritans saliva and the early host immune responses.ResultsThioglycolate-elicited peritoneal macrophages from BALB/c mice were stimulated by lipopolysaccharide (LPS) plus interferon-γ (IFN-γ) in the presence or absence of recombinant HTB. The presence of the salivary protein in the cultures inhibited nitric oxide production and decreased the inducible nitric oxide synthase (iNOS) expression induced by LPS plus IFN-γ. The tumor necrosis factor-α (TNF-α) and interleukin-12p40 (IL-12p40) levels were also reduced in the macrophages pre-incubated with HTB; these findings correlated to the decreased NF-κB expression. The biological activities described here were not associated with changes in annexin V binding to macrophages suggesting that HTB does not induce cell death. In addition, the activity of HTB seems to be specific to macrophages because no changes were observed in lymphocyte proliferation or cytokine production.ConclusionsWe describe here the first bioactive salivary protein of H. irritans. We characterized its ability to modulate macrophage inflammatory response, and the results can help explain how horn flies modulate the host immune system to feed on blood.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3017-z) contains supplementary material, which is available to authorized users.

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“…While the expression index of the endonucleases is relatively high (5-10), those of the serine proteases (0.1-0.2) and lipases (0.6-0.7) are relatively low. (Supplemental spreadsheet S1) coded for a truncated member of the antigen 5 family, previously described as a strong H. irritans salivary antigen [53], and had collected the highest number of reads, thus with an E. F. value of 100. The full transcript was recovered when the abyss and the trinity assemblies were further assembled together (Supplemental file F1), indicating this strategy to be the best to recover full transcripts of highly expressed messages.…”

Section: Ubiquitous Protein Families Overexpressed In H Irritans Salmentioning

confidence: 99%

An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

Ribeiro

Debat

Boiani

et al. 2019

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Background The horn fly (Haematobia irritans) is an obligate blood feeder that causes considerable economic losses in livestock industries worldwide. The control of this cattle pest is mainly based on insecticides; unfortunately in many regions, horn flies have developed resistance. Vaccines or biological control have been proposed as alternative control methods, but the available information about the biology or physiology of this parasite is rather scarce. Results We present a comprehensive description of the salivary and midgut transcriptomes of the horn fly (Haematobia irritans), using deep sequencing achieved by the Illumina protocol, as well as exploring the virome of this fly. Comparison of the two transcriptomes allow for identification of uniquely salivary or uniquely midgut transcripts, as identified by statistically differential transcript expression at a level of 16 x or more. In addition, we provide genomic highlights and phylogenetic insights of Haematobia irritans Nora virus and present evidence of a novel densovirus, both associated to midgut libraries of H. irritans. Conclusions We provide a catalog of protein sequences associated with the salivary glands and midgut of the horn fly that will be useful for vaccine design. Additionally, we discover two midgut-associated viruses that infect these flies in nature. Future studies should address the prevalence, biological effects and life cycles of these viruses, which could eventually lead to translational work oriented to the control of this economically important cattle pest.

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A Natural Cattle Immune Response Against Horn Fly (Diptera: Muscidae) Salivary Antigens May Regulate Parasite Blood Intake

Cited by 10 publications

References 23 publications

An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages

An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

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