A natural oil increases specific anti-OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide

Abstract

The efficacy of certain vaccines is improved by the use of adjuvants. Nowadays, the development of new, effective, and safe adjuvants that stimulate the innate immune response is researched. In this context, medicinal plants appear as a suitable alternative. Minthostachys verticillata essential oil (EO) has demonstrated the ability to modulate mechanisms of the innate immune response. Thus, the present work aimed to evaluate the EO adjuvant effect on humoral and cellular immunity, coadministered with OVA as antigen. The chemical analysis of EO by gas chromatographymass spectrometry revealed a predominant pulegone-menthone chemotype. EO (1.25, 2.5, or 5.0 mg/ml) did not alter the viability of murine fibroblasts (3T3 cell line) neither showed signs of toxicity in Balb/c mice inoculated subcutaneously. The serum of mice immunized with OVA + EO showed increased levels of anti-OVA-specific antibodies of IgG1 subclass compared with the mice immunized with OVA alone revealing an adjuvant effect of EO. The delayed type hypersensitivity showed that the combination OVA + Al(OH) 3 + EO was the best to induce a cellular immune response that extended until 48 h postinjection of OVA. M. verticillata EO appears as a new, safe, and effective adjuvant, which should continue to be studied for their possible future incorporation into vaccine formulations.

Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets

“…Different in vitro and in vivo studies carried out by our research group have demonstrated the ability of free M. verticillata EO to stimulate T cells (Cariddi et al 2011;Montironi et al 2019Montironi et al , 2022Campra et al 2021Campra et al , 2022. Here, we showed that the oral supplementation of EO (10 mg/kg/day) or NEO (10 mg/kg/day) increased the percentage of CD4 + T cells.…”

“…Furthermore, our research group has demonstrated the ability of EO to modulate the immune response in experimental mouse models administered intraperitoneally, subcutaneously, intramammary and orally. These discoveries contributed to the knowledge of a biological potential for EO not known until then (Cariddi et al 2006(Cariddi et al , 2007(Cariddi et al , 2011(Cariddi et al , 2013Montironi et al 2019Montironi et al , 2022Campra et al 2021Campra et al , 2022. In a recent study, we have reported the impact of EO oral administration on gastrointestinal and immune health in mice.…”

Influencia del estrés calórico en las reacciones adversas postinmunización en becerras Holstein-Friesian

“…La frecuencia de reacciones por parte de los lotes A, B y C, podría relacionarse con una mala manipulación del producto durante su fabricación, almacenamiento o transporte. Por otro lado, el hidróxido de aluminio como adyuvante también puede desencadenar alguna reacción adversa, sin embargo, la respuesta que produce es de hipersensibilidad tipo IV, es decir, una reacción retardada mediada por células Th1 que se manifiesta como nódulos subcutáneos pruriginosos persistentes en el lugar de la inyección (Nilsson et al 2017, Campra et al 2020.…”

Neutrophil Immunomodulatory Activity of Nerolidol, a Major Component of Essential Oils from Populus balsamifera Buds and Propolis

“…can modulate human neutrophil functions [ 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. Moreover, essential oils and some of their compounds (e.g., monoterpenes, sesquiterpenes) are reported to modulate humoral and cellular immune responses [ 37 , 38 , 39 , 40 , 41 , 42 ]. Thus, we hypothesized that essential oils from P. balsamifera buds and propolis collected nearby might be a novel source of anti-inflammatory compounds.…”