A natural promoter variation of <i>SlBBX31</i> confers enhanced cold tolerance during tomato domestication

Zhu, Yingfang; Zhu, Guangtao; Rui, Xu; Jiao, Zhixin; Yang, Junwei; Lin, Tao; Wang, Zhen; Huang, Sanwen; Chong, Leelyn; Zhu, Jian‐Kang

doi:10.1111/pbi.14016

Cited by 22 publications

(8 citation statements)

References 40 publications

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“…These studies indicate that BBX proteins are potentially involved in plant cold stress response. Recently, study has identified SlBBX31 as a positive regulator in tomato cold stress response (Zhu et al ., 2023). However, so far, the role and the underlying mechanisms of other SlBBX proteins in cold stress response in tomato plants remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

SlMPK1‐ and SlMPK2‐mediated SlBBX17 phosphorylation positively regulates CBF‐dependent cold tolerance in tomato

et al. 2023

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Summary B‐box (BBX) proteins are an important class of zinc finger transcription factors that play a critical role in plant growth and stress response. However, the mechanisms of how BBX proteins participate in the cold response in tomato remain unclear. Here, using approaches of reverse genetics, biochemical and molecular biology we characterized a BBX transcription factor, SlBBX17, which positively regulates cold tolerance in tomato (Solanum lycopersicum). Overexpressing SlBBX17 enhanced C‐repeat binding factor (CBF)‐dependent cold tolerance in tomato plants, whereas silencing SlBBX17 increased plant susceptibility to cold stress. Crucially, the positive role of SlBBX17 in CBF‐dependent cold tolerance was dependent on ELONGATED HYPOCOTYL5 (HY5). SlBBX17 physically interacted with SlHY5 to directly promote the protein stability of SlHY5 and subsequently increased the transcriptional activity of SlHY5 on SlCBF genes under cold stress. Further experiments showed that cold‐activated mitogen‐activated protein kinases, SlMPK1 and SlMPK2, also physically interact with and phosphorylate SlBBX17 to enhance the interaction between SlBBX17 and SlHY5, leading to enhanced CBF‐dependent cold tolerance. Collectively, the study unveiled a mechanistic framework by which SlMPK1/2‐SlBBX17‐SlHY5 regulated transcription of SlCBFs to enhance cold tolerance, thereby shedding light on the molecular mechanisms of how plants respond to cold stress via multiple transcription factors.

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Section: Introductionmentioning

confidence: 99%

SlMPK1‐ and SlMPK2‐mediated SlBBX17 phosphorylation positively regulates CBF‐dependent cold tolerance in tomato

et al. 2023

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“…tartaricum ’s high rutin content and self-reproduction [ 81 ]. In addition, a natural promoter variation of SlBBX31 improved cold tolerance during tomato domestication [ 82 ].…”

Section: Discussionmentioning

confidence: 99%

Haplotype-resolved genome of Prunus zhengheensis provides insight into its evolution and low temperature adaptation in apricot

Tan,

Zhou,

Huang

et al. 2024

Horticulture Research

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Prunus zhengheensis, an extremely rare population of apricots, originated in warm South-East China, is an excellent material for genetic breeding. However, most apricots and their two related species (P. sibirica, P. mandshurica) are found in the cold northern regions in China and the mechanism of their distribution is still unclear. In addition, the classification status of P. zhengheensis is controversial. Thus, we generated a high-quality haplotype-resolved genome for P. zhengheensis, exploring key genetic variations in its adaptation and the causes of phylogenetic incongruence. We found extensive phylogenetic discordances between the nuclear and organelle phylogenies of P. zhengheensis, which could be explained by incomplete lineage sorting. A 242.22 Mb pan-genome of Armeniaca section was developed with 13 chromosomal genomes. Importantly, we identified a 566 bp insertion in the promoter of the HSFA1d gene in apricot and showed that the activity of the HSFA1d promoter increased under low temperatures. In addition, HSFA1d overexpression in Arabidopsis thaliana indicated that HSFA1d positively regulated plant growth under chilling. Therefore, we hypothesised that the insertion in the promoter of HSFA1d in apricot improved its low temperature adaptation, allowing it to thrive in relatively cold locations. The findings help explain the weather adaptability of Armeniaca plants.

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“…Also, genetic variation in the promoter of a conserved leucine‐rich repeat receptor‐like kinase CTB4a (cold tolerance at booting), confers distinct levels of cold tolerance during temperate japonica evolution in cold habitats (Zhang et al, 2017). In tomato, it was reported that a 27‐bp InDel in the promoter of the CONSTANS‐like transcription factor SlBBX31, is linked to cold tolerance during tomato domestication (Zhu et al, 2023). In our study, all accessions harboring the CsSGR HapG allele were LT tolerant.…”

Section: Discussionmentioning

confidence: 99%

Natural variation in STAYGREEN contributes to low‐temperature tolerance in cucumber

Dong,

Li,

Tian

et al. 2023

JIPB

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Low temperature (LT) stress threatens cucumber production globally, however, the molecular mechanisms underlying LT tolerance in cucumber remain largely unknown. Here, using a genome wide association study (GWAS), we found a naturally occurring SNP in the STAYGREEN (CsSGR) coding region at the gLTT5.1 locus associated with LT tolerance. Knockout mutants of CsSGR generated by CRISPR/Cas9 exhibit enhanced LT tolerance, in particularly, increased chlorophyll content and reduced ROS accumulation in response to LT. Moreover, the C‐repeat Binding Factor 1 (CsCBF1) transcription factor can directly activate the expression of CsSGR. We demonstrate that the LT‐sensitive haplotype CsSGRHapA, but not the LT‐tolerant haplotype CsSGRHapG could interact with NON‐YELLOW COLORING 1 (CsNYC1) to mediate chlorophyll degradation. Geographic distribution of the CsSGR haplotypes indicated that the CsSGR HapG was selected in cucumber accessions from high latitudes, potentially contributing to LT tolerance during cucumber cold‐adaptation in these regions. CsSGR mutants also showed enhanced tolerance to salinity, water‐deficit, and Pseudoperonospora cubensis, thus CsSGR is an elite target gene for breeding cucumber varieties with broad‐spectrum stress tolerance. Collectively, our findings provide new insights into LT tolerance and will ultimately facilitate cucumber molecular breeding.This article is protected by copyright. All rights reserved.

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A natural promoter variation of SlBBX31 confers enhanced cold tolerance during tomato domestication

Cited by 22 publications

References 40 publications

SlMPK1‐ and SlMPK2‐mediated SlBBX17 phosphorylation positively regulates CBF‐dependent cold tolerance in tomato

SlMPK1‐ and SlMPK2‐mediated SlBBX17 phosphorylation positively regulates CBF‐dependent cold tolerance in tomato

Haplotype-resolved genome of Prunus zhengheensis provides insight into its evolution and low temperature adaptation in apricot

Natural variation in STAYGREEN contributes to low‐temperature tolerance in cucumber

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