A natural theaflavins preparation inhibits HIV-1 infection by targeting the entry step: Potential applications for preventing HIV-1 infection

Yang, Jie; Li, Lin; Tan, Suiyi; Jin, Hong; Qiu, Jiayin; Mao, Qin-Chao; Li, Runming; Xia, Chenglai; Jiang, Zhihong; Jiang, Shibo; Liu, Shuwen

doi:10.1016/j.fitote.2011.11.016

Cited by 53 publications

(32 citation statements)

References 27 publications

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“…TF-3 inactivates HSV at a neutral and acidic pH, is stable in this pH range (51), and when combined with LA at low pH inactivates HSV in the presence of semen and CVL. A microbicide that maintains low vaginal pH and provides additional LA to maintain a vaginal LA concentration of Ն1% (111 mM) is supplementing antiviral mechanisms already present in the vagina.…”

Section: Discussionmentioning

confidence: 98%

Theaflavin-3,3′-Digallate and Lactic Acid Combinations Reduce Herpes Simplex Virus Infectivity

Isaacs

2013

Antimicrob Agents Chemother

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The present study examined the efficacy of using multiple mechanisms as part of a topical microbicide to inactivate herpes simplex virus (HSV) by combining theaflavin-3,3=-digallate (TF-3) and lactic acid (LA) over the pH range of 4.0 to 5.7 to mimic conditions in the female reproductive tract. Six clinical isolates of HSV-2 and two clinical isolates of HSV-1 were almost completely inactivated when TF-3 (100 M) was present with LA over the pH range of 4.5 to 5.7, whereas four additional HSV-1 clinical isolates required TF-3 concentrations of 250 to 500 M for comparable virus titer reduction. LA (1%) alone at pH 4.0 reduced the titers of laboratory and clinical isolates of HSV-1 and HSV-2 by >5 log 10 , but most LA-dependent antiviral activity was lost at a pH of >4.5. When HSV-1 and HSV-2 were incubated at pH 4.0 without LA virus titers were not reduced. At pH 4.0, HSV-1 and HSV-2 titers were reduced 5 log 10 in 20 min by LA alone. TF-3 reduced HSV-2 titers by 5 log 10 in 20 to 30 min at pH 4.5, whereas HSV-1 required 60 min for comparable inactivation. Mixtures of TF-3 and LA stored at 37°C for 1 month at pH 4.0 to 5.7 maintained antiviral activity. Semen, but not cervical vaginal fluid, decreased LA-dependent antiviral activity at pH 4.0, but adding TF-3 to the mixture reduced HSV titers by 4 to 5 log 10 . These results indicate that a combination microbicide containing TF-3 and LA could reduce HSV transmission.

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Section: Discussionmentioning

confidence: 98%

Theaflavin-3,3′-Digallate and Lactic Acid Combinations Reduce Herpes Simplex Virus Infectivity

Isaacs

2013

Antimicrob Agents Chemother

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“…Notably, applications of viral entry/fusion inhibitors, such as anti‐HIV peptide T20 (enfuvirtide), either alone or in combination with other antivirus agents, have become an attractive antiviral strategy . Moreover, small molecules as potential entry inhibitors have been the object of research for many years . To date, however, no drug able to block viral entry is available for the treatment and prevention of influenza.…”

Section: The Role Of Namentioning

confidence: 99%

A new role of neuraminidase (NA) in the influenza virus life cycle: implication for developing NA inhibitors with novel mechanism of action

Yang

Liu

et al. 2016

Rev. Med. Virol.

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The entire life cycle of influenza virus involves viral attachment, entry, replication, and release. Previous studies have demonstrated that neuraminidase (NA) is an essential glycoprotein on the surface of influenza virus and that it is responsible for release of progeny virions from the host cell to infect new cells. However, recent studies have also suggested that NA may play other roles in the early stages of the viral life cycle, that is, viral attachment and entry. This review focuses on the new role of NA in the early stages of influenza life cycle and the corresponding development of novel NA inhibitors. Copyright © 2016 John Wiley & Sons, Ltd.

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“…According to molecular docking analysis TF3 may bind to a highly conserved hydrophobic pocket that is located on the surface of the central trimeric coiled-coil of glycoprotein 41 40 . Another in vitro investigation using p24 production and luciferase assays demonstrated that a natural preparation containing 90% theaflavins (TFmix) had a strongly anti-HIV-1 effect on both laboratory-adapted and primary HIV-1 strains possessing low cytotoxicity 75 . Inhibition occurred at viral entry of the host cell by targeting glycoprotein 41 and blocking membrane fusion.…”

Section: Black Tea Polyphenolsmentioning

confidence: 99%

Tea Polyphenols as Natural Products for Potential Future Management of HIV Infection - an overview

Sodagari

Bahramsoltani

Farzaei

et al. 2016

JNR

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Belonging to the <em>Lentivirus</em> genus of animal retroviruses, human immunodeficiency virus (HIV) is the etiological agent of acquired immunodeficiency syndrome (AIDS) which attacks cells of the immune system including CD4<sup>+</sup> T lymphocytes, monocytes, macrophages and dendritic cells. A rapid progression to immunodeficiency and the higher transmissibility of HIV-1 compared to HIV-2 are hallmarks of the worldwide spread of AIDS. Conventional HIV treatments are limited by drug toxicity and by multi-drug resistance, caused by the high genetic variability of HIV. This has led researchers into new areas of drug discovery in search of novel therapeutic molecules. Accumulating evidence indicates that tea polyphenols possess a range of beneficial properties including anti-cancer, anti-inflammatory, anti-oxidative, neuro-protective, anti-bacterial, anti-fungal and anti-viral effects. The anti-HIV infection potential of tea polyphenols has been confirmed by several preclinical studies. This suggests that polyphenol-rich extracts of tea could be used as dietary supplements as part of a combined therapeutic regimen with conventional anti-HIV drugs. Phenolic structures may also be considered as backbones for the discovery of a new generation of anti-HIV remedies. This review provides a perspective on the anti-HIV activity of tea polyphenols and their development as a possible source of future drugs for the therapy of HIV/AIDS.

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A natural theaflavins preparation inhibits HIV-1 infection by targeting the entry step: Potential applications for preventing HIV-1 infection

Cited by 53 publications

References 27 publications

Theaflavin-3,3′-Digallate and Lactic Acid Combinations Reduce Herpes Simplex Virus Infectivity

Theaflavin-3,3′-Digallate and Lactic Acid Combinations Reduce Herpes Simplex Virus Infectivity

A new role of neuraminidase (NA) in the influenza virus life cycle: implication for developing NA inhibitors with novel mechanism of action

Tea Polyphenols as Natural Products for Potential Future Management of HIV Infection - an overview

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