A natural uORF variant confers phosphorus acquisition diversity in soybean

Guo, Zilong; Cao, Hongrui; Zhao, Jing; Bai, Siqi; Peng, Wenting; Li, Jian; Sun, Lili; Chen, Liyu; Lin, Zhi‐Hao; Shi, Chen; Yang, Qing; Yang, Yongqing; Wang, Xiurong; Tian, Jichun; Chen, Zhi Chang; Liao, Hong

doi:10.1038/s41467-022-31555-2

Cited by 43 publications

(48 citation statements)

References 71 publications

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“…For example, alleles as natural allelic variation in haplotypes of GmINS1 and the P efficiency locus 1 could be the targets for breeding varieties with improvements in P efficiency and N fixation efficiency (Li et al ., 2018; Yang et al ., 2019). Recently, a natural uORF variant has been identified in the promoter region of GmPHF1 , which further confers higher translation efficiency of GmPTF1 protein and finally controls the polar localization of GmPT4 to the plasma membrane of root epidermal cells, and thus enhancing P acquisition efficiency (Guo et al ., 2022).…”

Section: Strategies To Improve P Use Efficiencies For High Snf In Leg...mentioning

confidence: 99%

“…Amon them, GmPT1 , GmPT4 , GmPT10 , GmPT12 , and GmPT13 showed high expressional levels in roots and have been implicated in P uptake by roots (Qin et al ., 2012a; Fan et al ., 2013). GmPT4 is localized on the root epidermal cells of soybean and contributes to the P uptake by roots (Guo et al ., 2022). On the contrary, some P transporters such as GmPT5 and GmPT10 are reported to be involved in P translocation from host roots to nodules in soybean (Qin et al ., 2012b; Lu et al ., 2020; Fig.…”

Section: Allocation Of P For Symbiotic N Fixationmentioning

confidence: 99%

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Cooperative interactions between nitrogen fixation and phosphorus nutrition in legumes

Zhong

Tian

et al. 2022

New Phytologist

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Summary Legumes such as soybean are considered important crops as they provide proteins and oils for humans and livestock around the world. Different from other crops, leguminous crops accumulate nitrogen (N) for plant growth through symbiotic nitrogen fixation (SNF) in coordination with rhizobia. A number of studies have shown that efficient SNF requires the cooperation of other nutrients, especially phosphorus (P), a nutrient deficient in most soils. During the last decades, great progress has been made in understanding the molecular mechanisms underlying the interactions between SNF and P nutrition, specifically through the identification of transporters involved in P transport to nodules and bacteroids, signal transduction, and regulation of P homeostasis in nodules. These studies revealed a distinct N–P interaction in leguminous crops, which is characterized by specific signaling cross talk between P and SNF. This review aimed to present an updated picture of the cross talk between N fixation and P nutrition in legumes, focusing on soybean as a model crop, and Medicago truncatula and Lotus japonicus as model plants. We also discuss the possibilities for enhancing SNF through improving P nutrition, which are important for high and sustainable production of leguminous crops.

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Section: Strategies To Improve P Use Efficiencies For High Snf In Leg...mentioning

confidence: 99%

Section: Allocation Of P For Symbiotic N Fixationmentioning

confidence: 99%

Cooperative interactions between nitrogen fixation and phosphorus nutrition in legumes

Zhong

Tian

et al. 2022

New Phytologist

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“…Some studies have suggested that loss of uORF function is associated with human disease ( 8 , 9 ). Studies in plants have found that the uORFs are associated with disease resistance, nutrient absorption and other traits ( 7 , 10 , 11 ). Due to the importance and widespread occurrence of uORFs, many studies have investigated the utilization of uORFs to manipulate translation and phenotypes ( 12–14 ).…”

Section: Introductionmentioning

confidence: 99%

A novel active transposon creates allelic variation through altered translation rate to influence protein abundance

Chen

Jiang

Dong

et al. 2023

Nucleic Acids Research

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Protein translation is tightly and precisely controlled by multiple mechanisms including upstream open reading frames (uORFs), but the origins of uORFs and their role in maize are largely unexplored. In this study, an active transposition event was identified during the propagation of maize inbred line B73. The transposon, which was named BTA for ‘B73 active transposable element hAT’, creates a novel dosage-dependent hypomorphic allele of the hexose transporter gene ZmSWEET4c through insertion within the coding sequence in the first exon, and results in reduced kernel size. The BTA insertion does not affect transcript abundance but reduces protein abundance of ZmSWEET4c, probably through the introduction of a uORF. Furthermore, the introduction of BTA sequence in the exon of other genes can regulate translation efficiency without affecting their mRNA levels. A transposon capture assay revealed 79 novel insertions for BTA and BTA-like elements. These insertion sites have typical euchromatin features, including low levels of DNA methylation and high levels of H3K27ac. A putative autonomous element that mobilizes BTA and BTA-like elements was identified. Together, our results suggest a transposon-based origin of uORFs and document a new role for transposable elements to influence protein abundance and phenotypic diversity by affecting the translation rate.

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“…Since symbiotic N fixation (SNF) in nodules requires significant inputs of energy, legumes are generally considered to have a high P requirement (Sulieman and Tran, 2015;Pang et al, 2018b;Zhong et al, 2023). Furthermore, as N-fixing root nodules are strong P sinks, the growth and yield of legumes are dramatically decreased by 30-40% under low P stress (Tesfaye et al, 2007;Valdeś-Loṕez and Hernańdez, 2008;Chen et al, 2011;Qin et al, 2012;Guo et al, 2022). Thus, low P availability is regarded as an important constraint for legume production.…”

Section: Introductionmentioning

confidence: 99%

Improving phosphorus acquisition efficiency through modification of root growth responses to phosphate starvation in legumes

Chen

Wang²,

Cardoso³

et al. 2023

Front. Plant Sci.

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Phosphorus (P) is one of the essential macronutrients for plant growth and development, and it is an integral part of the major organic components, including nucleic acids, proteins and phospholipids. Although total P is abundant in most soils, a large amount of P is not easily absorbed by plants. Inorganic phosphate (Pi) is the plant-available P, which is generally immobile and of low availability in soils. Hence, Pi starvation is a major constraint limiting plant growth and productivity. Enhancing plant P efficiency can be achieved by improving P acquisition efficiency (PAE) through modification of morpho-physiological and biochemical alteration in root traits that enable greater acquisition of external Pi from soils. Major advances have been made to dissect the mechanisms underlying plant adaptation to P deficiency, especially for legumes, which are considered important dietary sources for humans and livestock. This review aims to describe how legume root growth responds to Pi starvation, such as changes in the growth of primary root, lateral roots, root hairs and cluster roots. In particular, it summarizes the various strategies of legumes to confront P deficiency by regulating root traits that contribute towards improving PAE. Within these complex responses, a large number of Pi starvation-induced (PSI) genes and regulators involved in the developmental and biochemical alteration of root traits are highlighted. The involvement of key functional genes and regulators in remodeling root traits provides new opportunities for developing legume varieties with maximum PAE needed for regenerative agriculture.

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A natural uORF variant confers phosphorus acquisition diversity in soybean

Cited by 43 publications

References 71 publications

Cooperative interactions between nitrogen fixation and phosphorus nutrition in legumes

Cooperative interactions between nitrogen fixation and phosphorus nutrition in legumes

A novel active transposon creates allelic variation through altered translation rate to influence protein abundance

Improving phosphorus acquisition efficiency through modification of root growth responses to phosphate starvation in legumes

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