A nature-inspired colorimetric and fluorescent dual-modal biosensor for exosomes detection

Xia, Yaokun; Chen, Tingting; Chen, Guan-Yu; Weng, Yun-Ping; Zeng, Lupeng; Liao, Yijuan; Chen, Wenqian; Lan, Jianming; Zhang, Jing; Chen, Jinghua

doi:10.1016/j.talanta.2020.120851

Cited by 53 publications

(19 citation statements)

References 68 publications

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“…11 At present, the recognized standard methods widely used for exosome quan-tication and analysis include transmission electron microscopy (TEM), dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), atomic force microscopy (AFM), western blot (WB), enzyme-linked immunosorbent assay (ELISA), tunable resistive pulse sensing (RPS), ow cytometry and mass spectrometry (MS). [11][12][13][14] All of the aforementioned methods are used to analyze one characteristic aspect of exosomes, e.g. TEM is usually used to characterize the morphological characteristics, DLS and NTA are used to analyse the size and concentration of exosomes.…”

Section: Introductionmentioning

confidence: 99%

Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

et al. 2021

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“…During the past few years, various types of nanomaterials with unique optical properties have been extensively applied to develop sensitive biosensors for EV analysis [21,47]. Among the optical techniques, a promising nanotechnology-based colorimetric biosensor has been established to detect exosomal biomarkers with the naked eye based on the extinction coefficient [48][49][50]. In general, nanotechnology-based colorimetric biosensors can be characterized into two groups based on their different properties of nanomaterials: catalytic properties (i.e., nanozymes) and inherent optical properties [51].…”

Section: Colorimetric/fluorescence-based Analysis Methods For Ev Detementioning

confidence: 99%

Applications of Bionano Sensor for Extracellular Vesicles Analysis

2020

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Recently, extracellular vesicles (EVs) and their contents have been revealed to play crucial roles in the intrinsic intercellular communications and have received extensive attention as next-generation biomarkers for diagnosis of diseases such as cancers. However, due to the structural nature of the EVs, the precise isolation and characterization are extremely challenging. To this end, tremendous efforts have been made to develop bionano sensors for the precise and sensitive characterization of EVs from a complex biologic fluid. In this review, we will provide a detailed discussion of recently developed bionano sensors in which EVs analysis applications were achieved, typically in optical and electrochemical methods. We believe that the topics discussed in this review will be useful to provide a concise guideline in the development of bionano sensors for EVs monitoring in the future. The development of a novel strategy to monitor various bio/chemical materials from EVs will provide promising information to understand cellular activities in a more precise manner and accelerates research on both cancer and cell-based therapy.

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“…The signals were generated from fluorescein because of the catalyzed oxidation of 1,4-phenylenediamine. This biosensor is comparable to known methods, because it is sensitive with no interference from proteins, has a detection limit of ~3 × 10 3 particles/μL, and quantitatively measures the exosome extraction [ 229 ]. An advanced all-in-one biosensor was fabricated to simultaneously detect multiple biomarkers derived from breast cancer (miR-21, miR-27a, and miR-375) based on competitive strand displacement.…”

Section: Tumor Exosomes For Cancer Detectionsmentioning

confidence: 99%

Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques

2021

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Exosomes, powerful extracellular nanovesicles released from almost all types of living cells, are considered the communication engines (messengers) that control and reprogram physiological pathways inside target cells within a community or between different communities. The cell-like structure of these extracellular vesicles provides a protective environment for their proteins and DNA/RNA cargos, which serve as biomarkers for many malicious diseases, including infectious diseases and cancers. Cancer-derived exosomes control cancer metastasis, prognosis, and development. In addition to the unique structure of exosomes, their nanometer size and tendency of interacting with cells makes them a viable novel drug delivery solution. In recent years, numerous research efforts have been made to quantify and characterize disease-derived exosomes for diagnosis, monitoring, and therapeutic purposes. This review aims to (1) relate exosome biomarkers to their origins, (2) focus on current isolation and detection methods, (3) discuss and evaluate the proposed technologies deriving from exosome research for cancer treatment, and (4) form a conclusion about the prospects of the current exosome research.

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A nature-inspired colorimetric and fluorescent dual-modal biosensor for exosomes detection

Cited by 53 publications

References 68 publications

Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

Applications of Bionano Sensor for Extracellular Vesicles Analysis

Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques

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