A near-infrared analysis of water-macromolecule interactions: Hydration and the spectra of aqueous solutions of intact proteins

Vandermeulen, David L.; Ressler, Newton

doi:10.1016/0003-9861(80)90273-8

Cited by 24 publications

(13 citation statements)

References 15 publications

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“…For intermediate cases (0 < θ < 1) the supposition [29] leads to the assumption that decrease of confusion and elevation of spatial order in location of components results in a rise of the value of the index θ or χ. Thereby, the notion of dielectric order has been introduced for disperse systems, and this notion was related directly with the value of the index of power law (6) or (7) for dielectric permittivity of the considered system [29].…”

Section: Theoretical Model Of Bulk Grain Dielectric Permittivitymentioning

confidence: 99%

“…According to Equation (6), in the limiting case of moisture absence in kernels (M → 0), their dielectric permittivity ε k (ε k → ε dk ) should coincide with the permittivity ε ks : ε ks = ε dk . Then in this case for the dielectric permittivity of the solid substance ε sm from (7) we obtain…”

Section: Theoretical Model Of Bulk Grain Dielectric Permittivitymentioning

confidence: 99%

“…where ε (exp) gj are the values of bulk grain dielectric permittivity which were measured in experiment [15] for one frequency and N various values of moisture content M j (N = 15), ε g (M j ) are the values of the same quantity, which are calculated theoretically for the same values of moisture content M j through the use of Equations (1), (2), (4)- (8), (10)- (12) after substitution of relations (15), (16) into (6). The minimum of the functional (17) will be sought by varying coefficients a wm and calculating the sums over all measured values of moisture content [15] for every frequency.…”

Section: Dielectric Properties Of Bound Watermentioning

confidence: 99%

“…In particular, it was established by this way that water within porous disperse systems is characterized by specific properties differing from the properties of ordinary free water (socalled bound water) [5][6][7][8][9][10][11]. It is known that near a solid surface or close to a polymeric macromolecule, water forms the ice-like cluster structure [7,10,12].…”

Section: Introductionmentioning

confidence: 99%

“…Some researchers regard that the boundary values of its local density and dielectric permittivity in the monomolecular interface layer are identical to the properties of ice [9,10]. Other authors indicate on differences between these water phases [6,8], pointing out in particular to the existence of the Debye relaxation frequency for bound water near 100 MHz [8], whereas the similar frequency for ice is about 10 kHz [13,14]. Probably, the following point of view is correct according to which the properties of bound water are not the same for different heterogeneous systems and are dependent on their composition.…”

Section: Introductionmentioning

confidence: 99%

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Dielectric Study of Bound Water in Grain at Radio and Microwave Frequencies

Serdyuk

2008

PIER

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Abstract-A phenomenological theoretical model of grain dielectric properties is presented for radio and microwave frequencies. On the bases of this model, an inverse problem is solved to determine the dielectric permittivity of bound water inside kernels of winter wheat using results of complex permittivity measurements for bulk grain carried out by other authors. The character of the water complex permittivity dependence on frequency and kernel moisture content is studied. For this, the permittivity was considered as a sum of five different functions, depending on moisture content explicitly, and with coefficients being subject to determination. The frequency dependence of these coefficients was analyzed, and the regions typical for the ionic conductivity and for the dipole-orientational polarization mechanism were detected. For this polarization mechanism, the relaxation frequencies are differ from those of free water. It was concluded that water microparticles inside kernels are formed not by pure water, but by aqueous solution of four different substances coming from the kernel solid phase. It is shown that bound water, for the most part, is in a state that is intermediate between that of free water and that of molecules in the monomolecular water layer on the solid phase boundary.

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Section: Theoretical Model Of Bulk Grain Dielectric Permittivitymentioning

confidence: 99%

Section: Theoretical Model Of Bulk Grain Dielectric Permittivitymentioning

confidence: 99%

Section: Dielectric Properties Of Bound Watermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dielectric Study of Bound Water in Grain at Radio and Microwave Frequencies

Serdyuk

2008

PIER

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Thermodynamic functions of biopolymer hydration. I. Their determination by vapor pressure studies, discussed in an analysis of the primary hydration process

Lüscher-Mattli

Rüegg

1982

Biopolymers

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SynopsisThe primary hydration process of native biopolymers is analyzed in a brief review of the literature, pertaining to various aspects of biopolymer-water systems. Based on this analysis, a hydration model is proposed that implies that the solution conformation of native biopolymers is stable at and above a critical degree of hydration (hp, = 0.060.1 g HzO/g polymer). This water content corresponds to the fraction of strongly bound water, and amounts to -20% of the primary hydration sphere. In order to test this model, detailed sorption-desorption scanning experiments were performed on a globular protein (a-chymotrypsin). The results obtained are consistent with the proposed hydration model. They show that under certain experimental conditions, sorption isotherms can be obtained that do not exhibit hysteresis. These data represent equilibrium conditions and are thus accessible to thermodynamic treatment. Valid thermodynamic functions, pertinent to the interaction of water with biopolymers in their solution state, can be obtained from these sorption experiments.

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