A near-infrared BSA coated DNA-AgNCs for cellular imaging

Mu, Wei-Yu; Yang, Rui; Akrofi, Robertson; Chen, Qiuyun

doi:10.1016/j.colsurfb.2017.12.023

Cited by 23 publications

(6 citation statements)

References 25 publications

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“…To verify the universality of this method, we attempted to synthesize other types of metal NCs using a DNR-assisted technique. The nucleic acid bases have been found to form coordination with many metal ions, such as silver (Ag) . Thus, we synthesized DNRs/AgNCs by the same approach, and more details about the preparation and characterization of the AgNCs are given in the Supporting Information (Figures S13–S17).…”

Section: Resultsmentioning

confidence: 99%

DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging

Ouyang,

Jia,

Luo

et al. 2023

JACS Au

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Metal nanoclusters (NCs) have emerged as a promising class of fluorescent probes for cellular imaging due to their high resistance to photobleaching and low toxicity. Nevertheless, their widespread use in clinical diagnosis is limited by their unstable intracellular fluorescence. In this study, we develop an intracellularly biosynthesized fluorescent probe, DNA nanoribbon-gold NCs (DNR/AuNCs), for longterm cellular tracking. Our results show that DNR/AuNCs exhibit a 4-fold enhancement of intracellular fluorescence intensity compared to free AuNCs. We also investigated the mechanism underlying the fluorescence enhancement of AuNCs by DNRs. Our findings suggest that the higher synthesis efficiency and stability of AuNCs in the lysosome may contribute to their fluorescence enhancement, which enables long-term (up to 15 days) fluorescence imaging of cancer cells (enhancement of ∼60 times compared to free AuNCs). Furthermore, we observe similar results with other metal NCs, confirming the generality of the DNR-assisted biosynthesis approach for preparing highly bright and stable fluorescent metal NCs for cancer cell imaging.

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Section: Resultsmentioning

confidence: 99%

DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging

Ouyang,

Jia,

Luo

et al. 2023

JACS Au

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“…Peak b at 570 nm indicated the formation of ultrasmall Ag nanoclusters wrapped by DNA templates . While peak a represented the large Ag nanoparticles due to the aggregation of silver atoms . In addition, the formation was verified by fluorescence spectra (Figure C, black and red curves).…”

Section: Resultsmentioning

confidence: 99%

“…31 While peak a represented the large Ag nanoparticles due to the aggregation of silver atoms. 32 In addition, the formation was verified by fluorescence spectra (Figure 1C, black and red curves). Upon excitation at 510 nm, the DNA-AgNCs showed a maximum fluorescence emission at 570 nm, suggesting the available fluorescence properties of AgNCs, which were consistent with published studies.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

CRISPR/Cas Precisely Regulated DNA-Templated Silver Nanocluster Fluorescence Sensor for Meat Adulteration Detection

Chen

Liu

et al. 2022

J. Agric. Food Chem.

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Meat adulteration can cause consumer fraud, food allergies, and religious issues. Rapid and sensitive detection methods are urgently demanded to supervise meat authenticity. Herein, a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas precisely regulated DNA-templated silver nanocluster (DNA-AgNC) sensor was ingeniously designed to detect meat adulteration. Specific sequence recognition of CRISPR/Cas12a allowed accurate identification of target DNA. The emerging label-free fluorescent probes, DNA-AgNCs, a class of promising fluorophores in biochemical analysis with attractive photostability and remarkably enhanced fluorescence properties, were first introduced as the substrates of CRISPR/Cas12a system, allowing a sensitive output of amplified signals through the precise regulation of the unique target DNA-activated trans-cleavage activity of Cas12a. Based on this specific recognition, efficient signal transduction of CRISPR/Cas12a, and the outstanding fluorescence properties of DNA-AgNCs, the proposed strategy achieved a satisfactory linear range from 10 pM to 1 μM with a limit of detection (LOD) as low as 1.9 pM, which can achieve sensitive detection of meat adulteration.

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“…To remove dyes from textile fibers and recover uncolored (white) materials, Mu and Yang 109 used different solvents for PA6.6, as well as PET and cotton. Different number of treatment (cycles) were applied for each system.…”

Section: Physical Recyclingmentioning

confidence: 99%

Recycling of polyamides: Processes and conditions

Hirschberg

Rodrigue

2023

Journal of Polymer Science

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Polyamides (PA) are a family of engineering thermoplastics used in a wide range of applications including automotive, building, construction, separation processes, textiles, and so forth. This is related to their good properties (mechanical and thermal) which can also be modified by additives, other polymers (blends and multilayers) and fillers (composites). But these complex systems (several components) make the final materials more difficult to recycle. In this review, basic statistics on the production rate of polyamides are presented and the different recycling methods are reported to compare their advantages and limitations with respect to economics and technical analyses. The discussion includes biological, chemical, mechanical, physical, and thermal treatments to reintroduce, as much as possible, the parts after their end‐of‐service. Finally, a general conclusion on the current state of PA recycling is presented with several openings for future developments to satisfy the concept of circular economy and general sustainability.

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A near-infrared BSA coated DNA-AgNCs for cellular imaging

Cited by 23 publications

References 25 publications

DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging

DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging

CRISPR/Cas Precisely Regulated DNA-Templated Silver Nanocluster Fluorescence Sensor for Meat Adulteration Detection

Recycling of polyamides: Processes and conditions

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