A near-infrared fluorescent long-chain fatty acid toward optical imaging of cardiac metabolism in living mice

Swamy, Mahadeva M. M.; Zubir, Mohamad Zarif Mohd; Mutmainah,; Tsuboi, Setsuko; Murai, Yuta; Monde, Kenji; Hirano, Ken‐ichi

doi:10.1039/d2an00999d

Cited by 1 publication

(4 citation statements)

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“…Therefore, it has been established as a test for TGCV [3-5]. In a previous study, while utilizing the advantages of 123 I-BMIPP, we developed an Alexa680-labeled BMIPP skeleton [10]. In the present study, we focused on the import and export ability of fluorescently-labeled BMIPP skeletons (fluorescent BMPP) in cultured cells.…”

Section: Discussionmentioning

confidence: 99%

“…First, fluorescent BMPP is imported into cells depending on its BMIPP skeleton, which has been shown to be incorporated into the TG pool in cardiomyocytes in vivo [25]. Second, fluorescent BMPP has been reported to be imported into and exported from the hearts of mice depending on the BMIPP skeleton in vivo [10]. Third, 123 I-BMIPP has been shown to be useful for examining LCFA flux under various heart conditions in humans [26,27].…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescently-labeled BMPPs (fluorescent BMPP) in phosphate buffered saline (PBS) were kindly gifted by Professors Kenji Monde and Takashi Jin, and were prepared from amino-BMPP as described in a previous report [10]. All experiments were performed at least twice.…”

Section: Treatment Of Cells With Fluorescently-labeled Bmppmentioning

confidence: 99%

“…Therefore, we focused on developing a novel fluorescently-labeled BMIPP-basic structure without using a radioisotope. In a previous study, we synthesized a BMIPP-based fluorescent LCFA analog, in which iodine-123 was replaced with Alexa Fluor 680 [10]. In this study, we investigated the characteristics of fluorescently-labeled BMIPP skeleton to detect FA metabolism in cultured cells.…”

Section: Introductionmentioning

confidence: 99%

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A novel fluorescently-labeled long-chain fatty acid analog for the study of fatty acid metabolism in cultured cells

Hara

Hirano

2022

Preprint

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123I-15-(p-iodophenyl)-(R,S)-methyl pentadecanoic acid (BMIPP) is a long-chain fatty acid (LCFA) analog developed to examine myocardial LCFA metabolism and has been used as a tracer for nuclear cardiology. However, its use is limited because of the specialized features of cardiac scintigraphy. In this study, a novel BMIPP-based probe was utilized, in which iodine-123 was replaced with a fluorescent compound, to extend the use of 123I-BMIPP to a wider variety of cells ex vivo. To confirm that this fluorescent LCFA analog (fluorescent BMPP) was imported into cells, fluorescence-activated cell sorting (FACS) analysis and fluorescent cell imaging were performed using cultured cells. The analysis showed that the import of fluorescent BMPP into the cells occurred in a concentration-dependent manner. This import into cells was inhibited by Sulfosuccinimidyl Oleate in a dose-dependent manner, which is an inhibitor of CD36, a well-known LCFA transporter, suggesting that fluorescent BMPP could be imported into cells via the same pathway as LCFA. FACS and cell imaging intensities of the cells importing fluorescent BMPP were attenuated after incubation in the non-Alexa680-BMPP medium. These results suggest that fluorescent BMPP can be transported into and from cells, reflecting the metabolism of LCFA. Fluorescently-labeled BMPP has the potential to be used as a probe for studying LCFA metabolism in various cells.

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Section: Discussionmentioning

confidence: 99%