A near-infrared light triggered fluormetric biosensor for sensitive detection of acetylcholinesterase activity based on NaErF4: 0.5 % Ho3+@NaYF4 upconversion nano-probe

Zhao, Xu; Zhang, Ling; Yan, Xu; Zhang, Li; Lü, Yang; Pan, Jialin; Zhang, Meiling; Wang, Chenguang; Suo, Hui; Jia, Xiaoteng; Liu, Xiaomin; Lu, Geyu

doi:10.1016/j.talanta.2021.122784

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…The R channel, which is the most sensitive for indigo color recording, was proved to be the best choice for an assay of neurotoxic inhibitors of cholinesterase. Regarding the limits of detection, the presented biosensor does not provide an improvement to the recently introduced biosensors for pesticides as those examples are described in the quoted papers [ 15 , 26 – 28 ]. The colorimetric biosensor performed here does not outperform the other biosensors in the limit of detection and sensitivity but brings significant improvement in simplicity and potency to be readily manufacturable with acceptable analytical specifications.…”

Section: Resultsmentioning

confidence: 99%

“…The inhibition of AChE by various compounds was used in many analytical procedures like an assay of diazinon by AChE electrochemical biosensor [ 13 ], an assay of galantamine by a microcalorimetry AChE biosensor [ 14 ], a near-infrared light fluorometric biosensor for AChE activity assay [ 15 ], and screen-printed amperometric biosensor with immobilized AChE detecting phenylmethylsulfonylfluoride [ 16 ]. The authors of the cited papers chose performance for one inhibitor, but the devices are sensitive to multiple inhibitors.…”

Section: Introductionmentioning

confidence: 99%

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A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

Pohanka

Zakova

2022

International Journal of Analytical Chemistry

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Biosensors containing cholinesterase are analytical devices suitable for the assay of neurotoxic compounds. In the research on biosensors, a new platform has appeared some years ago. It is the digital photography and scoring of coloration (photogrammetry). In this paper, a colorimetric biosensor is constructed using 3D-printed multiwell pads treated with indoxylacetate as a chromogenic substrate and gold nanoparticles with the immobilized enzyme butyrylcholinesterase. A smartphone camera served for photogrammetry. The biosensor was tested for the assay of carbofuran and paraoxon ethyl as two types of covalently binding inhibitors: irreversible and pseudoirreversible. The biosensor exerted good sensitivity to the inhibitors and was able to detect carbofuran with a limit of detection for carbofuran 7.7 nmol/l and 17.6 nmol/l for paraoxon ethyl. A sample sized 25 μl was suitable for the assay lasting approximately 70 minutes. Up to 121 samples can be measured contemporary using one multiwell pad. The received data fully correlated with the standard spectrophotometry. The colorimetric biosensor exerts promising specifications and appears to be competitive to the other analytical procedures working on the principle of cholinesterase inhibition. Low-cost, simple, and portable design represent an advantage of the assay of the biosensor. Despite the overall simplicity, the biosensor can fully replace the standard spectroscopic methods.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

Pohanka

Zakova

2022

International Journal of Analytical Chemistry

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“…Over the past few years, many research efforts focused on developing fluorescent nanosensors for ChE activity and inhibition, − some of which were able to provide spatiotemporal resolution. − , Fluorescent sensors hold promise for superior optical selectivity over the more traditional absorbance-based methods when measuring in the presence of biological media, , while spatiotemporal-resolving sensors offer exciting possibilities over the more traditional bulk measurements. For example, such spatiotemporal information can potentially serve as tumor resection guides due to cholinesterase activity difference between brain glioma cells and normal brain cells .…”

Section: Introductionmentioning

confidence: 99%

Monitoring the Activity and Inhibition of Cholinesterase Enzymes using Single-Walled Carbon Nanotube Fluorescent Sensors

2022

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Cholinesterase enzymes are involved in a wide range of bodily functions, and their disruption is linked to pathologies such as neurodegenerative diseases and cancer. While cholinesterase inhibitors are used as drug treatments for diseases such as Alzheimer and dementia at therapeutic doses, acute exposure to high doses, found in pesticides and nerve agents, can be lethal. Therefore, measuring cholinesterase activity is important for numerous applications ranging from the search for novel treatments for neurodegenerative disorders to the on-site detection of potential health hazards. Here, we present the development of a near-infrared (near-IR) fluorescent single-walled carbon nanotube (SWCNT) optical sensor for cholinesterase activity and demonstrate the detection of both acetylcholinesterase and butyrylcholinesterase, as well as their inhibition. We show sub U L–1 sensitivity, demonstrate the optical response at the level of individual nanosensors, and showcase an optical signal output in the 900–1400 nm range, which overlaps with the biological transparency window. To the best of our knowledge, this is the longest wavelength cholinesterase activity sensor reported to date. Our near-IR fluorescence-based approach opens new avenues for spatiotemporal-resolved detection of cholinesterase activity, with numerous applications such as advancing the research of the cholinergic system, detecting on-site potential health hazards, and measuring biomarkers in real-time.

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“…The nanoparticles spread in the varnish environment uniformly without any agglomeration. We have not used any surface modification with organic compounds or additional shells to obtain intense visible emission activated by two different excitation wavelengths and identified on a surface painted in any color 28 – 30 . Thanks to it, the obtained effect of the anti-counterfeiting materials is satisfying, but the structure is not so complicated as well as the synthesis process.…”

Section: Introductionmentioning

confidence: 99%

Anti-counterfeiting system based on luminescent varnish enriched by NIR- excited nanoparticles for paper security

Przybylska

Grzyb

Érdman

et al. 2022

Sci Rep

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Up-converting nanoparticles can be a demand for requirements in many areas, including bioimaging and conversion of energy, but also in the battle against counterfeiting. The properties of lanthanide ions make falsification difficult or even impossible using appropriately designed systems. The proposition of such an approach is the NaErF4:Tm3+@NaYF4 core@shell up-converting nanoparticles combined with transparent varnishes. Given the spectroscopic properties of Er3+ ions present in the fluoride matrix, the obtained up-converting nanoparticles absorb light by 808 and 975 nm wavelengths. The intentionally co-doped Tm3+ ions enable tuning characteristic green Er3+ emission to red luminescence, particularly desirable in anti-counterfeiting applications. The article includes a thorough analysis of structural and morphological properties. Moreover, this work shows that exclusive luminescent properties of NaErF4:Tm3+@NaYF4 NPs can be given to the transparent varnish, providing an excellent anti-counterfeiting system, revealing red emission under two different excitation wavelengths.

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A near-infrared light triggered fluormetric biosensor for sensitive detection of acetylcholinesterase activity based on NaErF4: 0.5 % Ho3+@NaYF4 upconversion nano-probe

Cited by 14 publications

References 47 publications

A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

Monitoring the Activity and Inhibition of Cholinesterase Enzymes using Single-Walled Carbon Nanotube Fluorescent Sensors

Anti-counterfeiting system based on luminescent varnish enriched by NIR- excited nanoparticles for paper security

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