A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model

Wu, Yongquan; Shi, Aiping; Li, Yuanyan; Zeng, Hong; Chen, Xiaoyong; Wu, Jie; Fan, Xiaolin

doi:10.1039/c8an01107a

Cited by 37 publications

(27 citation statements)

References 60 publications

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“…With a high detection limit, the nanoprobe can only be activated by the elevated production of ONOO − at the inflammation site but it cannot be activated by the normal generation of ONOO − in other tissues, showing a selective inflammation imaging capacity. Wu et al designed a xanthene fluorescent probe with NIR emission 20 . In this work, the fluorescence of the probe is prequenched, and via a nucleophilic addition‐elimination pathway, diphenyl phosphinate, the fluorescence quencher of this probe, can be removed by peroxynitrite, hence realizing the imaging of inflammation in response to peroxynitrite.…”

Section: Strategies For Visualizing Inflammationsmentioning

confidence: 99%

Strategies for visualizing inflammation

Zhang

Liu

Guo

et al. 2020

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As a basic innate immune response to the disordered tissue homeostasis, inflammation is related to the pathogenesis of multiple diseases, including bacterial infections, atherosclerosis, neurodegenerative diseases, and cancers. It is also a pivotal feature of some metabolic disorders such as diabetes and obesity. The visualization of in vivo inflammations can help us to comprehend the pathogenesis of these diseases and develop new solutions to diagnose them. Over the past few decades, a variety of strategies (eg, computed tomography, magnetic resonance imaging [MRI], and ultrasound [US] imaging) have been utilized for visualizing inflammations by imaging the structural changes of inflammatory tissues. Moreover, many recent studies have focused on some probes that can target or localize the inflammatory sites by specific binding to inflammation‐related molecules, being internalized by inflammatory cells, or becoming detectable only under inflammatory conditions. These probes can also be applied to visualize inflammations by MRI, positron emission tomography, single‐photon emission computed tomography, photoacoustic imaging, optical imaging (eg, fluorescence imaging, bioluminescence imaging, and chemiluminescence imaging), contrast‐enhanced US imaging, and the combined use of the abovementioned methods. This review not only summarizes the existing strategies for visualizing inflammations, but also discusses the limitations of the present strategies and the future directions on the development of new strategies for realizing the in vivo inflammation visualization.

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Section: Strategies For Visualizing Inflammationsmentioning

confidence: 99%

Strategies for visualizing inflammation

Zhang

Liu

Guo

et al. 2020

VIEW

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“…Recently, a near-infrared (NIR, 650–900 nm) fluorescence imaging method featured with deep imaging depth, noninvasive visualization, and high sensitivity has become the cutting-edge method for the study of targets in vivo . − Though a growing body of well-designed fluorescence probes for ONOO – has been raised and some complex roles of ONOO – in cells and living bodies were elucidated, − there are only a handful of NIR fluorescent probes intentionally constructed for the in vivo monitoring of ONOO – content in inflammation progress, which exhibited limited sensitivity and S/N ratio. − Additionally, some organic NIR fluorophores were reported to be easily oxidized by ONOO – due to the strong oxidizing nature of ONOO – , which may result in false-negative signals and inaccurate detection results. − In this scenario, developing an NIR probe for sensitive in vivo detection of ONOO – fluxes in inflammation is still pressing.…”

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confidence: 99%

Highly Sensitive Near-Infrared Imaging of Peroxynitrite Fluxes in Inflammation Progress

Wang

et al. 2021

Anal. Chem.

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Inflammation is an important protection reaction in living organisms associated with many diseases. Since peroxynitrite (ONOO − ) is engaged in the inflammatory processes, illustrating the key nexus between ONOO − and inflammation is significant. Due to the lack of sensitive ONOO − in vivo detection methods, the research still remains at its infancy. Herein, a highly sensitive NIR fluorescence probe DDAO-PN for in vivo detection of ONOO − in inflammation progress was reported. The probe responded to ONOO − with significant NIR fluorescence enhancement at 657 nm (84-fold) within 30 s in solution. Intracellular imaging of exogenous ONOO − with the probe demonstrated a 68-fold fluorescence increase (F/F 0 ). Impressively, the probe can in vivo detect ONOO − fluxes in LPS-induced rear leg inflammation with a 4.0-fold fluorescence increase and LPS-induced peritonitis with an 8.0-fold fluorescence increase The remarkable fluorescence enhancement and quick response enabled real-time tracking of in vivo ONOO − with a large signal-to-noise (S/N) ratio. These results clearly denoted that DDAO-PN was able to be a NIR fluorescence probe for in vivo detection and high-fidelity imaging of ONOO − with high sensitivity and will boost the research of inflammation-related diseases.

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“…8,9 Recent advances in reaction-based small-molecule uorescent probes provide the possibility to track elusive small biomolecules in their local environments. 10,11 Actually, for targeting ONOO À , there have been several robust probes reported that were conrmed for their specicity and capability to image nitrosative stress in the context of inammation, [12][13][14][15][16] kidney injury, 17 hepatotoxicity, [18][19][20][21] and heart disease. 22,23 However, the context of the neurovascular unit is more formidable due to the presence of the blood-brain barrier (BBB).…”

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confidence: 99%

Physicochemical-property guided design of a highly sensitive probe to image nitrosative stress in the pathology of stroke

Cheng

Sun

et al. 2020

Chem. Sci.

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A near-infrared xanthene fluorescence probe for monitoring peroxynitrite in living cells and mouse inflammation model

Abstract: A novel near-infrared xanthene fluorescence probe for monitoring peroxynitrite in vitro and in vivo.

Cited by 37 publications

References 60 publications

Strategies for visualizing inflammation

Strategies for visualizing inflammation

Highly Sensitive Near-Infrared Imaging of Peroxynitrite Fluxes in Inflammation Progress

Physicochemical-property guided design of a highly sensitive probe to image nitrosative stress in the pathology of stroke

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