A network pharmacological approach to investigate the pharmacological effects of CZ2HF decoction on Alzheimer's disease

Wei, Yu; Gao, Jianmei; Xu, Fan; Shi, Jingshan; Yu, Chunhao; Gong, Qihai

doi:10.1002/j.2769-2795.2021.tb00080.x

Cited by 7 publications

(8 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further explore the potential mechanism and key targets of MFS in treating AD, we conducted network pharmacology and molecular docking analysis on drug component action targets and disease pathogenic targets to explore the possible mechanism of MFSs in treating AD ( Figure 4 ) ( Wei, et al, 2021 ). Through the aforementioned research, we found that MFSs have a therapeutic effect on AD.…”

Section: Resultsmentioning

confidence: 99%

Miracle fruit seed as a potential supplement for the treatment of learning and memory disorders in Alzheimer’s disease

Huang

Xue²,

Chen³

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

Currently, the treatment of Alzheimer’s disease (AD) is still at the stage of symptomatic treatment due to lack of effective drugs. The research on miracle fruit seeds (MFSs) has focused on lipid-lowering and antidiabetic effects, but no therapeutic effects have been reported in AD. The purpose of this study was to provide data resources and a potential drug for treatment of AD. An AD mouse model was established and treated with MFSs for 1 month. The Morris water maze test was used to assess learning memory function in mice. Nissl staining was used to demonstrate histopathological changes. MFSs were found to have therapeutic implications in the AD mouse model, as evidenced by improved learning memory function and an increase in surviving neurons. To explore the mechanism of MFSs in treating AD, network pharmacological approaches, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and molecular docking studies were carried out. Based on the network pharmacology strategy, 74 components from MFS corresponded to 293 targets related to the AD pathology. Among these targets, AKT1, MAPK3, ESR1, PPARG, PTGS2, EGFR, PPARA, CNR1, ABCB1, and MAPT were identified as the core targets. According to the relevant number of core targets, cis-8-octadecenoic acid, cis-10-octadecenoic acid, 2-dodecenal, and tetradecane are likely to be highly correlated with MFS for AD. Enrichment analysis indicated the common targets mainly enriched in AD and the neurodegeneration-multiple disease signaling pathway. The molecular docking predictions showed that MFSs were stably bound to core targets, specifically AKT1, EGFR, ESR1, PPARA, and PPARG. MFSs may play a therapeutic role in AD by affecting the insulin signaling pathway and the Wnt pathway. The findings of this study provide potential possibilities and drug candidates for the treatment of AD.

show abstract

Section: Resultsmentioning

confidence: 99%

Miracle fruit seed as a potential supplement for the treatment of learning and memory disorders in Alzheimer’s disease

Huang

Xue²,

Chen³

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…After combining with previous results and the network analysis, apoptosis related factor (Bax, bcl-2 and caspase3) was chosen for further validation in the common genes. A large amount of evidence shows that the network pharmacology exerts a crucial role in the treatment of the nervous system, which provides an important basis for mechanism study 30 , 31 .…”

Section: Discussionmentioning

confidence: 99%

The neuroprotective effects of Lutongkeli in traumatic brain injury rats by anti-apoptosis mechanism

Xiao

Xue

et al. 2022

Acta Cir. Bras.

View full text Add to dashboard Cite

To explore the neuroprotective effects of Lutongkeli (LTKL) in traumatic brain injury (TBI) and detect the related mechanism. Methods: TBI model was established with LTKL administration (2 and 4 g/kg/d, p.o.). Motor function of rats was examined by Rotarod test. Nissl staining was used to show neuron morphology. Furthermore, the disease-medicine common targets were obtained with the network pharmacology and analyzed with Kyoto Encyclopedia of Genes and Genomes. Lastly, the predicted targets were validated by real-time polymerase chain reaction. Results: After LTKL administration, neural behavior was significantly improved, and the number of spared neurons in brain was largely increased. Moreover, 68 bioactive compounds were identified, corresponding to 148 LTKL targets; 2,855 genes were closely associated with TBI, of which 87 overlapped with the LTKL targets and were considered to be therapeutically relevant. Functional enrichment analysis suggested LTKL exerted its pharmacological effects in TBI by modulating multiple pathways including apoptosis, inflammation, etc. Lastly, we found LTKL administration could increase the mRNA level of Bcl-2 and decrease the expression of Bax and caspase-3. Conclusion: This study reported the neuroprotective effect of LTKL against TBI is accompanied with anti-apoptosis mechanism, which provides a scientific explanation for the clinical application of LTKL in the treatment of TBI.

show abstract

“…The molecular docking of related neurodegenerative diseases also confirmed this. 52 Thus, we speculate that quercetin, kaempferol, β-sitosterol, and stigmasterol are active ingredients regulating most of the…”

Section: The Three Pathway-related Target Genesmentioning

confidence: 96%

An exploratory study on the mechanism of Huangqi Guizhi Wuwu Decoction in the treatment of neuropathic pain

Luo

Zhou

Xiao

et al. 2022

Ibrain

View full text Add to dashboard Cite

Huangqi Guizhi Wuwu Decoction (HGWD) has a definite effect on neuropathic pain (NP), whereas the specific mechanism has not been elucidated. The components and targets in HGWD were collected and identified through System Pharmacology Database (Traditional Chinese Medicine Database and Analysis Platform). Genecards and Online Mendelian Inheritance in Man databases were used to search for NP-related genes.The Venn diagram was drawn to get the intersection target. Cytoscape 3.8.0 software was used to construct the compound-disease-target-pathway networks. STRING database was applied to analyze protein-protein interaction of potential targets. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses were used to identify the function of genes related to NP. Finally, molecular docking was performed to visualize the binding mode and affinity between proteins and active ingredients.According to the intersection target of the Venn diagram, the network graph is constructed by Cytoscape and the results show the five compounds, β-sitosterol, (+)-catechin, quercetin, Stigmasterol, kaempferol, and 15 genes (CASP3, FOS, GSK3B, HSP90AA1, IKBKB, IL6, MAPK8, RELA, ICAM1, SELE, ELK1, HSPB1, PRKACA, PRKCA, RAF1) were highly correlated with NP. KEGG and GO of 15 genes results that TNF, IL-17 and MAPK signaling pathway were Significantly related to the pathological mechanism of NP. Molecular docking showed that core genes in this network were IL-6 (TNF and IL-17 signaling pathways), ICAM1 (TNF signaling pathway), and CASP3 (three signal pathways). This study found that the five active compounds, three core genes, and three signaling pathways may be the key to the treatment of NP by HGWD.

show abstract

A network pharmacological approach to investigate the pharmacological effects of CZ2HF decoction on Alzheimer's disease

Cited by 7 publications

References 46 publications

Miracle fruit seed as a potential supplement for the treatment of learning and memory disorders in Alzheimer’s disease

Miracle fruit seed as a potential supplement for the treatment of learning and memory disorders in Alzheimer’s disease

The neuroprotective effects of Lutongkeli in traumatic brain injury rats by anti-apoptosis mechanism

An exploratory study on the mechanism of Huangqi Guizhi Wuwu Decoction in the treatment of neuropathic pain

Contact Info

Product

Resources

About