A network pharmacology approach to explore and validate the potential targets underlying the effect of Ginsenoside on Osteoporosis

Abstract

Objective This study aim to investigate the potential targets involving the effect of ginsenoside on osteoporosis using a network pharmacology approach. Methods Ginsenoside and its drug targets associated to osteoporosis (OP) were identified by using network analysis. First, the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), DrugBank database, Pharmmapper database and Cytoscape software were used to mine information relevant to Ginsenoside ingredients and Ginsenoside -related targets. Second, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of ginsenoside-target gene and ginsenoside-OP target gene were performed in String, Phenopedia, DisGeNET database and Metascape software. Eventually, the protein to protein interaction (PPI) of key ginsenoside-OP targets were created by String database and Cytoscape software. The validation of the binding of ginsenoside to target proteins was plotted by utilizing SwissDock tool, UCSF Chimera and Pymol software. Results A total of 8 important active ingredients of ginsenosides were obtained in the TCMSP. Eighty potential targets of ginsenoside and 1304 related targets involved in OP were subjected to network analysis, and the 17 intersection targets were indicated to be linked to ginsenoside treating OP. GO and KEGG analysis showed the top 10 items of biological processes, cellular components, molecular functions and signaling pathways in the 80 targets of ginsenoside. Then, 14 key targets were determined to be the most crucial genes by protein to protein interaction (PPI) analysis. In the 14 intersection potential targets, 10 signaling pathways were defined by Metascape software. Validation plots of four target proteins IL1B, TNF, IFNG, NFKBIA binding to ginsenoside rh2 was drew, lastly. Conclusion This study investigated the potential targets and signaling pathways of ginsenoside during the treatment of OP, which might be beneficial to elucidate the mechanism concerned to the action of ginsenoside and might supply a better understanding of its anti-OP effects.