A neuroglial cooperativity is required for the potentiation by 2- chloroadenosine of the muscarinic-sensitive phospholipase C in the striatum

El‐Etr, Martine; Cordier, Jocelyne; Głowiński, J.; Prémont, Joël

doi:10.1523/jneurosci.09-05-01473.1989

Cited by 86 publications

(79 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Primary neuronal cultures of embryonic day 17 fetuses were carried out as described by El-Etr et al (16). In brief, dissociated cells were plated (200,000 cells per cm 2 ) on glass coverslips coated with poly(L-ornithine) and cultured in a completely defined medium in 5% CO 2 at 37°C.…”

Section: Control Of Binding Protein Enrichmentmentioning

confidence: 99%

Pregnenolone binds to microtubule-associated protein 2 and stimulates microtubule assembly

Murakami

Fellous

Baulieu

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

129

View full text Add to dashboard Cite

CorrectionsNEUROBIOLOGY. For the article ''Coupling between changes in human brain temperature and oxidative metabolism during prolonged visual stimulation'' by Dmitriy A. Yablonskiy, Joseph J. H. Ackerman, and Marcus E. Raichle, which appeared in number 13, June 20, 2000, of Proc. Natl. Acad. Sci. USA (97, 7603-7608), the authors note a correction in the second to last paragraph on page 7604. The sentence ''It is well known from positron-emission tomography (PET) data (17, 18) that rCMRO 2 maps in the normal human brain are largely flat despite considerable regional differences in rCBF and rCMRO 2 maps'' should read: ''It is well known from positron-emission tomography (PET) data (17,18) MAP2), which was increased more than 8-fold after copolymerization of MAP2 with tubulin. Finally, steroid effects on microtubule-assembly kinetics were assayed. Pregnenolone induced a large, dose-related increase of both the rate and extent of MAP2-induced tubulin assembly, whereas progesterone, inactive per se, counteracted the stimulatory effect of pregnenolone. Electron microscopic analysis confirmed that pregnenolone-increased assembly of microtubules produced a completely normal structure. The stimulatory effect on MAP2-tubulin interaction was also observed in fetal rat-brain neuron cultures. Therefore, we propose a mechanism of neurosteroid action, the control of microtubule or, more generally, of neural cytoskeleton dynamics, with potential roles in brain development, plasticity, and aging.T he characterization of pregnenolone (PREG) and of dehydroepiandrosterone (DHEA) in the rat brain as nonconjugated steroids and their sulfate and fatty acid esters has led to the discovery of a steroid biosynthetic pathway in the central nervous system. Nongenomic, membrane receptor-mediated activities have been documented for PREG sulfate (PREGS) and DHEA sulfate (DHEAS). They are prototypic, naturally excitatory neurosteroids, because at low-micromolar concentrations, they antagonize ␥-aminobutyric acid type A receptors or potentiate glutamate receptors of the N-methyl-D-aspartate type, and they may modulate other neurotransmitter receptors (1-3).However, no intracellular binding protein has been reported so far in the nervous system for DHEA or PREG. In this paper, we have investigated PREG binding sites in rat-brain cytosol. The search for potential binding proteins in cytosol preparations led to the discovery of a PREG binding site on microtubuleassociated protein 2 (MAP2), which has important roles in the nervous system.Neurons have a characteristic morphology of neuritic processes densely filled with parallel arrays of microtubules, which play an essential role in the growth and maintenance of neurites during neuronal differentiation (4, 5). Neuronal microtubules are composed of tubulin and of several nontubulin proteins that purify with tubulin throughout several courses of polymerization-depolymerization (6). The proteins present in in vitropolymerized microtubules from brain extracts have been characterized. Tubulin is a pro...

show abstract

Section: Control Of Binding Protein Enrichmentmentioning

confidence: 99%

Pregnenolone binds to microtubule-associated protein 2 and stimulates microtubule assembly

Murakami

Fellous

Baulieu

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

129

View full text Add to dashboard Cite

show abstract

“…:>us neurotransmitters in the regulation of both cyclic AMP and inositol phosphate formation (EI-Etr et al, 1989;Hili and Kendall, 1987;Hol1ingsworth et al, 1986). The blockade by CBZ of A 1 -and possibly also A 2 b-receptors should inhibi t these synergistic effects of adenosine and could t"ereby profoundly alter the regulation by various neurotransmitters of both second messenger systems.…”

Section: Discussionmentioning

confidence: 99%

Carbamazepine distinguishes between adenosine receptors that mediate different second messenger responses

Calker¹,

Steber²,

Klotz

et al. 1991

European Journal of Pharmacology: Molecular Pharmacology

View full text Add to dashboard Cite

The mechanism of the therapeutic and prophylactic effects of carbamazepine (CBZ) in affective psychose.s is unknown but may in part be related to the potent competitive interaction of CBZ with adenosine-binding sites in the brain. The antioonvuls4l:At and sedative properties of CBZ are reminiscent of the effects evoked by adenosine-agonists and contrast sharply with the opposite aclions of adenosine-antagonists like caffeine. However. indirect evidence suggests an antagonist-rather than an agonist-like activity of CBZ at adenosi11e-receptors. We have used various model systems, in which adenosine receptor subtypes mediate different second messenger-responses, to investigate this apparent paradox. CBZ was found to antagonize the Ai·ret:eptor-mediated inhibition of cydic AMP accumulation in cultured astroblasts and in GH 3 -cells. Furthermore, CBZ also inh.ibits the adenosi.ne-induced increase in the Ievel of cyclic ".MP in cultured astrobla.sts, which is med.iated by low-affinity A 2 b-receptors. ln contrast, CBZ does not block the inhibition elicited by adenosine-agonists of the agonist-induced increased formation of inositolphosphates in human neutrophils, which is mediated by high-affinity A 23 -receptors. The specific antagonism by CBZ of A 1 -but not of high-affinity A 23 -receptors was further supported by binding experiments using rat brain membranes. These results suggest tbat the paradox of CBZ's antagonistic effects at adenosine-receptors might be at least partially reconciled by a selective anta%onistic action of CBZ at A 1 recertors but not at high-affinity A2a-receptors.

show abstract

“…Astrocyte Cultures-Primary cultures of mouse striatal astrocytes were prepared as described previously (23). Briefly striata from 18-day-old Swiss mouse embryos were mechanically dissociated in PBS supplemented with 33 mM glucose (PBS-glucose).…”

Section: Methodsmentioning

confidence: 99%

Difference in Mass Analysis Using Labeled Lysines (DIMAL-K)

Delcourt

Jouin

Poncet

et al. 2005

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Here we describe an original strategy for unbiased quantification of protein expression called difference in mass analysis using labeled lysine (K) (DIMAL-K). DIMAL-K is based on the differential predigestion labeling of lysine residues in complex protein mixtures. The method is relevant for proteomic analysis by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Protein labeling on lysine residues uses two closely related chemical reagents, S-methyl thioacetimidate and S-methyl thiopropionimidate. Using protein standards, we demonstrated that 1) the chemical labeling was quantitative, specific, and rapid; 2) the differentially labeled proteins co-migrated on two-dimensional gels; and 3) the identification by mass fingerprinting and the relative quantification of the proteins were possible from a single MALDI-TOF mass spectrum. The power of the method was tested by comparing and quantifying the secretion of proteins in normal and proinflammatory astrocytic secretomes (20 g). We showed that DIMAL-K was more sensitive and accurate than densitometric image analysis and allowed the detection and quantification of novel proteins. Molecular & Cellular Proteomics 4:1085-1094, 2005.Since the last decade, MS has become one of the most powerful techniques in proteomics. Two-dimensional (2-D) 1 PAGE in combination with MALDI-TOF analysis provides a sensitive method for protein identification. An important challenge in proteomics deals with quantification of relative expression levels of individual proteins in two different biological samples. In this context, quantification by mass spectrometry has recently become a major alternative strategy to analysis based on densitometric or fluorescence technologies (1-4). Despite extensive work accomplished in the field of quantification, only few strategies have been associated with the widely used 2-D PAGE/MALDI.Quantification by mass spectrometry requires the production of differentially labeled peptides from two sets of proteins. The difference in expression levels is obtained by measuring the relative intensities of MS signals of the mixed labeled peptides. Different strategies have already been described (for reviews, see Refs. 2 and 5-8). These strategies differ in 1) the pre-or postdigestion labeling, 2) the choice of the labeled function (thiol, primary amine, or carboxylic acid), 3) the use of stable isotopes or alternative chemicals, and 4) the type of MS analysis (LC-MS/MS or MALDI-TOF MS). Predigestion labeling allows for greater quantification accuracy than postdigestion labeling. Indeed introducing the chemical modification early in the experimental procedure reduces differential loss of proteins during further biochemical steps and guarantees accuracy of the quantification. Predigestion labeling often involves stable isotope incorporation through either metabolic labeling or chemical labeling on a specific residue of proteins. Metabolic labeling, such as the stable isotope labeling by amino acids in cell culture (SILAC) method, is introduced in vivo ear...

show abstract

A neuroglial cooperativity is required for the potentiation by 2- chloroadenosine of the muscarinic-sensitive phospholipase C in the striatum

Cited by 86 publications

References 17 publications

Pregnenolone binds to microtubule-associated protein 2 and stimulates microtubule assembly

Pregnenolone binds to microtubule-associated protein 2 and stimulates microtubule assembly

Carbamazepine distinguishes between adenosine receptors that mediate different second messenger responses

Difference in Mass Analysis Using Labeled Lysines (DIMAL-K)

Contact Info

Product

Resources

About